Restriction map for
pFN21AB5783
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AccIII AcyI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
Bst1107I BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MunI NarI
NdeI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 157 GGTACC G^GTACC
AclI 550 AACGTT AA^CGTT
AhdI 819 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 511 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 157 GGTACC G^GTACC
AspEI 819 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 157 GGTACC G^G[CT][GA]CC
BbsI 319 GAAGAC GAAGAC(2/6)
BpuAI 319 GAAGAC GAAGAC(2/6)
BsaWI 1743 ACCGGA [AT]^CCGG[AT]
BsrFI 1179 GCCGGC [GA]^CCGG[CT]
BssHII 56 GCGCGC G^CGCGC
BssSI 337 CTCGTG CACGAG(-5/-1)
BstBI 115 TTCGAA TT^CGAA
BstXI 574 CCATCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1056 GGATCT [GA]^GATC[CT]
Cfr10I 1179 GCCGGC [GA]^CCGG[CT]
DsaI 1805 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
Eam1105I 819 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1925 CCTTACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 741 AGCGCC [GA]GCGC^[CT]
HincII 553 GTTGAC GT[CT]^[GA]AC
HindII 553 GTTGAC GT[CT]^[GA]AC
KpnI 157 GGTACC GGTAC^C
MflI 1056 GGATCT [GA]^GATC[CT]
MslI 575 CATCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1179 GCCGGC GCC^GGC
NcoI 1805 CCATGG C^CATGG
NgoMIV 1179 GCCGGC G^CCGGC
NheI 1824 GCTAGC G^CTAGC
NspI 206 GCATGC [GA]CATG^[CT]
NspV 115 TTCGAA TT^CGAA
Psp1406I 550 AACGTT AA^CGTT
SbfI 1093 CCTGCAGG CCTGCA^GG
SfuI 115 TTCGAA TT^CGAA
SphI 206 GCATGC GCATG^C
Sse8387I 1093 CCTGCAGG CCTGCA^GG
XhoII 1056 GGATCT [GA]^GATC[CT]
XmnI 511 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 514 GAATTT [GA]^AATT[CT]
1908 AAATTC
AlwI 1069 GATCC GGATC(4/5)
1056 GGATC
ApaI 1457 GGGCCC GGGCC^C
1725 GGGCCC
ApoI 514 GAATTT [GA]^AATT[CT]
1908 AAATTC
AspHI 468 GAGCTC G[AT]GC[AT]^C
1585 GAGCTC
BciVI 363 GGATAC GTATCC(6/5)
1221 GGATAC
BclI 1397 TGATCA T^GATCA
1974 TGATCA
BcnI 1502 CCGGG CC^[GC]GG
1723 CCGGG
BfaI 424 CTAG C^TAG
1825 CTAG
BglI 715 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1804 GCCATGGGGGC
BsaI 1699 GAGACC GGTCTC(1/5)
2030 GAGACC
BsiHKAI 468 GAGCTC G[AT]GC[AT]^C
1585 GAGCTC
BsmBI 624 CGTCTC CGTCTC(1/5)
851 CGTCTC
BsrDI 768 CATTGC GCAATG(2/0)
1136 CATTGC
BstAPI 1096 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1558 GCAGAGAGTGC
BstEII 703 GGTGACC G^GT[ACGT]ACC
875 GGTCACC
BstPI 703 GGTGACC G^GT[ACGT]ACC
875 GGTCACC
EcoO65I 703 GGTGACC G^GT[ACGT]ACC
875 GGTCACC
FbaI 1397 TGATCA T^GATCA
1974 TGATCA
MaeI 424 CTAG C^TAG
1825 CTAG
MspA1I 1424 CAGCTG C[AC]G^C[GT]G
1448 CAGCTG
NciI 1502 CCGGG CC^[GC]GG
1723 CCGGG
PflMI 75 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
447 CCACCATCTGG
PvuII 1424 CAGCTG CAG^CTG
1448 CAGCTG
SacI 468 GAGCTC GAGCT^C
1585 GAGCTC
Van91I 75 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
447 CCACCATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AocI AvaI AvaII BanII BbvCI
BbvI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I