Restriction map for
pFN21AE2182
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I TspRI Tth111I
Van91I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 48 GTATAC GT^[AC][GT]AC
AccII 72 CGCG CG^CG
AccIII 437 TCCGGA T^CCGGA
AciI 447 GCGG CCGC(-3/-1)
AlwI 297 GGATC GGATC(4/5)
AocI 58 CCTGAGG CC^T[ACGT]AGG
BalI 428 TGGCCA TGG^CCA
BanII 235 GGGCTC G[GA]GC[CT]^C
BbsI 393 GAAGAC GAAGAC(2/6)
BfaI 172 CTAG C^TAG
BmyI 235 GGGCTC G[AGT]GC[ACT]^C
BpuAI 393 GAAGAC GAAGAC(2/6)
BsaWI 437 TCCGGA [AT]^CCGG[AT]
BseAI 437 TCCGGA T^CCGGA
BsmI 518 GAATGC GAATGC(1/-1)
Bsp1286I 235 GGGCTC G[AGT]GC[ACT]^C
BspEI 437 TCCGGA T^CCGGA
BspMI 142 GCAGGT ACCTGC(4/8)
BsrI 294 ACTGG ACTGG(1/-1)
Bst1107I 48 GTATAC GTA^TAC
BstEII 145 GGTGACC G^GT[ACGT]ACC
BstPI 145 GGTGACC G^GT[ACGT]ACC
BstUI 72 CGCG CG^CG
BstXI 257 CCAACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 297 GGATCT [GA]^GATC[CT]
BstZ17I 48 GTATAC GTA^TAC
Bsu36I 58 CCTGAGG CC^T[ACGT]AGG
Eco81I 58 CCTGAGG CC^T[ACGT]AGG
EcoNI 4 CCTAAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 145 GGTGACC G^GT[ACGT]ACC
EcoT14I 407 CCAAGG C^C[AT][AT]GG
FauI 447 GCGGG CCCGC(4/6)
HgaI 70 GACGC GACGC(5/10)
HinfI 175 GACTC G^A[ACGT]TC
HphI 145 GGTGA GGTGA(8/7)
MaeI 172 CTAG C^TAG
MflI 297 GGATCT [GA]^GATC[CT]
MluNI 428 TGGCCA TGG^CCA
MroI 437 TCCGGA T^CCGGA
MscI 428 TGGCCA TGG^CCA
MslI 372 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 72 CGCG CG^CG
NlaIII 372 CATG CATG^
PleI 175 GACTC GAGTC(4/5)
PvuII 76 CAGCTG CAG^CTG
SfaNI 496 GATGC GCATC(5/9)
StyI 407 CCAAGG C^C[AT][AT]GG
XbaI 171 TCTAGA T^CTAGA
XcmI 407 CCAAGGCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 297 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AluI 77 AGCT AG^CT
317 AGCT
BglI 26 GCCACAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
421 GCCCGGCTGGC
BsaJI 101 CCAGGG C^C[ACGT][ACGT]GG
407 CCAAGG
BstF5I 435 CATCC GGATG(2/0)
192 GGATG
BstNI 101 CCAGG CC^[AT]GG
417 CCTGG
CfoI 73 GCGC GCG^C
219 GCGC
Cfr13I 307 GGGCC G^G[ACGT]CC
420 GGCCC
DpnII 66 GATC ^GATC
298 GATC
EaeI 386 TGGCCG [CT]^GGCC[GA]
428 TGGCCA
EcoRII 101 CCAGG ^CC[AT]GG
417 CCTGG
FokI 435 CATCC GGATG(9/13)
192 GGATG
HhaI 73 GCGC GCG^C
219 GCGC
HinP1I 73 GCGC G^CGC
219 GCGC
MboI 66 GATC ^GATC
298 GATC
MspA1I 76 CAGCTG C[AC]G^C[GT]G
445 CAGCGG
MvaI 101 CCAGG CC^[AT]GG
417 CCTGG
NdeII 66 GATC ^GATC
298 GATC
PstI 79 CTGCAG CTGCA^G
140 CTGCAG
Sau3AI 66 GATC ^GATC
298 GATC
Sau96I 307 GGGCC G^G[ACGT]CC
420 GGCCC
SfcI 79 CTGCAG C^T[GA][CT]AG
140 CTGCAG
SfiI 25 GGCCACAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
420 GGCCCGGCTGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
BbvI BcnI BseRI BsiYI BslI
BssKI Cac8I DdeI EarI Fnu4HI
HaeIII HapII HpaII ItaI Ksp632I
MaeIII MboII MnlI MspI MwoI
NciI ScrFI TaqI TseI Tsp45I
TthHB8I