Restriction map for
pFN21AE1945
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BbrPI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NarI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI Van91I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 420 GTATAC GT^[AC][GT]AC
AcsI 1120 GAATTC [GA]^AATT[CT]
AfaI 1440 GTAC GT^AC
AflIII 686 ACATGT A^C[GA][CT]GT
Alw44I 1378 GTGCAC G^TGCAC
AlwNI 468 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1044 CCTCAGG CC^T[ACGT]AGG
ApaLI 1378 GTGCAC G^TGCAC
ApoI 1120 GAATTC [GA]^AATT[CT]
AspI 586 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 937 CTCGAG C^[CT]CG[GA]G
BbsI 607 GTCTTC GAAGAC(2/6)
BbvCI 1431 CCTCAGC CCTCAGC(-5/-2)
BciVI 944 GTATCC GTATCC(6/5)
BclI 1397 TGATCA T^GATCA
BfaI 1003 CTAG C^TAG
BpuAI 607 GTCTTC GAAGAC(2/6)
BsaWI 752 ACCGGA [AT]^CCGG[AT]
BsiEI 630 CGACCG CG[GA][CT]^CG
BsoBI 937 CTCGAG C^[CT]CG[GA]G
BspLU11I 686 ACATGT A^CATGT
Bst1107I 420 GTATAC GTA^TAC
BstEII 1138 GGTGACC G^GT[ACGT]ACC
BstPI 1138 GGTGACC G^GT[ACGT]ACC
BstXI 1097 CCATGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1415 AGATCC [GA]^GATC[CT]
BstZ17I 420 GTATAC GTA^TAC
Bsu36I 1044 CCTCAGG CC^T[ACGT]AGG
DraI 1210 TTTAAA TTT^AAA
DraIII 1468 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1471 GACGTGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1044 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1138 GGTGACC G^GT[ACGT]ACC
EcoRI 1120 GAATTC G^AATTC
FbaI 1397 TGATCA T^GATCA
HaeII 552 GGCGCT [GA]GCGC^[CT]
HincII 1342 GTCAAC GT[CT]^[GA]AC
HindII 1342 GTCAAC GT[CT]^[GA]AC
KspI 927 CCGCGG CCGC^GG
MaeI 1003 CTAG C^TAG
MflI 1415 AGATCC [GA]^GATC[CT]
MseI 1211 TTAA T^TAA
NaeI 523 GCCGGC GCC^GGC
NcoI 1103 CCATGG C^CATGG
NgoMIV 523 GCCGGC G^CCGGC
PaeR7I 937 CTCGAG C^TCGAG
PflFI 586 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 864 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 835 CTGCAG CTGCA^G
RsaI 1440 GTAC GT^AC
SacII 927 CCGCGG CCGC^GG
SapI 888 GCTCTTC GCTCTTC(1/4)
SexAI 78 ACCTGGT A^CC[AT]GGT
SfiI 738 GGCCAGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SwaI 1209 ATTTAAAT ATTT^AAAT
Tru9I 1211 TTAA T^TAA
Tth111I 586 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1002 TCTAGA T^CTAGA
XcmI 299 CCATCTTCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 937 CTCGAG C^TCGAG
XhoII 1415 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 1416 GATCC GGATC(4/5)
1240 GGATC
ApaI 746 GGGCCC GGGCC^C
824 GGGCCC
BalI 737 TGGCCA TGG^CCA
1094 TGGCCA
BcgI 282 CGACTTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
272 GCACCAGGCTCG
BglI 730 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
739 GCCAGGCGGGC
BsaI 1178 GAGACC GGTCTC(1/5)
487 GGTCTC
BsmFI 674 GGGAC GGGAC(10/14)
866 GTCCC
BsrFI 523 GCCGGC [GA]^CCGG[CT]
632 ACCGGC
BssSI 162 CACGAG CACGAG(-5/-1)
478 CACGAG
CfoI 13 GCGC GCG^C
553 GCGC
Cfr10I 523 GCCGGC [GA]^CCGG[CT]
632 ACCGGC
DraII 824 GGGCCCT [GA]G^G[ACGT]CC[CT]
864 GGGTCCC
EcoO109I 824 GGGCCCT [GA]G^G[ACGT]CC[CT]
864 GGGTCCC
EcoT14I 128 CCTTGG C^C[AT][AT]GG
1103 CCATGG
HgaI 250 GACGC GACGC(5/10)
590 GCGTC
HhaI 13 GCGC GCG^C
553 GCGC
HinP1I 13 GCGC G^CGC
553 GCGC
MaeII 1134 ACGT A^CGT
1472 ACGT
MluNI 737 TGGCCA TGG^CCA
1094 TGGCCA
MscI 737 TGGCCA TGG^CCA
1094 TGGCCA
NspI 686 ACATGT [GA]CATG^[CT]
1292 ACATGC
PleI 378 GACTC GAGTC(4/5)
534 GACTC
SacI 394 GAGCTC GAGCT^C
934 GAGCTC
SmlI 898 CTCAAG C^T[CT][GA]AG
937 CTCGAG
StyI 128 CCTTGG C^C[AT][AT]GG
1103 CCATGG
TfiI 903 GAATC G^A[AT]TC
1223 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanII BbvI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I