Restriction map for
pFN21AB7848
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BsaI BsaWI BseAI BseRI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI Eco47III
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MroI MseI MunI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfiI SfuI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflIII 116 ACGTGT A^C[GA][CT]GT
AhdI 1064 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 304 CCTGAGG CC^T[ACGT]AGG
AspEI 1064 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1355 TGGCCA TGG^CCA
BbsI 450 GTCTTC GAAGAC(2/6)
BpuAI 450 GTCTTC GAAGAC(2/6)
BsaAI 1780 TACGTG [CT]AC^GT[GA]
BsiWI 1754 CGTACG C^GTACG
BspHI 161 TCATGA T^CATGA
BssHII 1588 GCGCGC G^CGCGC
BssSI 494 CTCGTG CACGAG(-5/-1)
BstEII 1302 GGTGACC G^GT[ACGT]ACC
BstPI 1302 GGTGACC G^GT[ACGT]ACC
BstXI 1082 CCATTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 304 CCTGAGG CC^T[ACGT]AGG
DraIII 484 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1064 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 304 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1302 GGTGACC G^GT[ACGT]ACC
EcoT14I 1186 CCATGG C^C[AT][AT]GG
HgaI 1531 GCGTC GACGC(5/10)
MluNI 1355 TGGCCA TGG^CCA
MscI 1355 TGGCCA TGG^CCA
MslI 1083 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1186 CCATGG C^CATGG
NspI 942 GCATGC [GA]CATG^[CT]
PshAI 372 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 161 TCATGA T^CATGA
SacI 1327 GAGCTC GAGCT^C
ScaI 476 AGTACT AGT^ACT
SgrAI 1825 CGCCGGTG C[GA]^CCGG[CT]G
SphI 942 GCATGC GCATG^C
StyI 1186 CCATGG C^C[AT][AT]GG
TaqI 312 TCGA T^CGA
TthHB8I 312 TCGA T^CGA
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 893 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1786 CAGAAACTG
AspHI 66 GAGCAC G[AT]GC[AT]^C
1327 GAGCTC
BfaI 505 CTAG C^TAG
1763 CTAG
BlpI 81 GCTGAGC GC^T[ACGT]AGC
810 GCTCAGC
Bpu1102I 81 GCTGAGC GC^T[ACGT]AGC
810 GCTCAGC
BsiHKAI 66 GAGCAC G[AT]GC[AT]^C
1327 GAGCTC
BsmAI 370 GAGAC GTCTC(1/5)
391 GTCTC
BspMI 1181 ACCTGC ACCTGC(4/8)
651 GCAGGT
CelII 81 GCTGAGC GC^T[ACGT]AGC
810 GCTCAGC
EarI 13 GAAGAG CTCTTC(1/4)
1644 GAAGAG
Ksp632I 13 GAAGAG CTCTTC(1/4)
1644 GAAGAG
KspI 1396 CCGCGG CCGC^GG
1719 CCGCGG
MaeI 505 CTAG C^TAG
1763 CTAG
NaeI 1166 GCCGGC GCC^GGC
1422 GCCGGC
NgoMIV 1166 GCCGGC G^CCGGC
1422 GCCGGC
NotI 903 GCGGCCGC GC^GGCCGC
1398 GCGGCCGC
PflMI 621 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
992 CCACCTACTGG
PpuMI 836 GGGACCT [GA]G^G[AT]CC[CT]
1286 AGGACCC
PstI 649 CTGCAG CTGCA^G
877 CTGCAG
PvuII 374 CAGCTG CAG^CTG
1459 CAGCTG
SacII 1396 CCGCGG CCGC^GG
1719 CCGCGG
SapI 13 GAAGAGC GCTCTTC(1/4)
1644 GAAGAGC
SexAI 323 ACCTGGT A^CC[AT]GGT
485 ACCAGGT
SfaNI 1452 GATGC GCATC(5/9)
1581 GCATC
SfcI 649 CTGCAG C^T[GA][CT]AG
877 CTGCAG
SmlI 425 CTCAAG C^T[CT][GA]AG
472 CTCAAG
Van91I 621 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
992 CCACCTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BsgI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfoI
SmaI TseI Tsp45I TspRI XhoII
XmaCI XmaI