Restriction map for
pFN21AE2125
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BanI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tsp45I Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 762 CGCG CG^CG
AcsI 382 AAATTC [GA]^AATT[CT]
AfaI 540 GTAC GT^AC
ApoI 382 AAATTC [GA]^AATT[CT]
BamHI 45 GGATCC G^GATCC
BanII 137 GGGCTC G[GA]GC[CT]^C
BcgI 468 CGAGGGCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 858 CCGGG CC^[GC]GG
BglII 423 AGATCT A^GATCT
BmyI 137 GGGCTC G[AGT]GC[ACT]^C
BsgI 841 GTGCAG GTGCAG(16/14)
BsmAI 610 GAGAC GTCTC(1/5)
BsmBI 610 GAGACG CGTCTC(1/5)
Bsp1286I 137 GGGCTC G[AGT]GC[ACT]^C
BspMI 615 GCAGGT ACCTGC(4/8)
BssHII 761 GCGCGC G^CGCGC
BstUI 762 CGCG CG^CG
BstXI 548 CCAAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 786 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 282 CCATGG C^C[GA][CT]GG
EcoO109I 786 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 520 AGCGCC [GA]GCGC^[CT]
HapII 858 CCGG C^CGG
HpaII 858 CCGG C^CGG
MaeIII 677 GTTAC ^GT[ACGT]AC
MslI 238 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 91 CAGCGG C[AC]G^C[GT]G
MspI 858 CCGG C^CGG
MvnI 762 CGCG CG^CG
NciI 858 CCGGG CC^[GC]GG
NcoI 282 CCATGG C^CATGG
PflMI 255 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 540 GTAC GT^AC
ScaI 539 AGTACT AGT^ACT
SmlI 337 CTTGAG C^T[CT][GA]AG
StuI 812 AGGCCT AGG^CCT
Tsp509I 383 AATT ^AATT
Van91I 255 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 256 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
593 CAGGGTCTG
AocI 688 CCTAAGG CC^T[ACGT]AGG
850 CCTGAGG
AvaI 466 CCCGAG C^[CT]CG[GA]G
718 CCCGAG
AvaII 798 GGACC G^G[AT]CC
834 GGACC
BalI 87 TGGCCA TGG^CCA
746 TGGCCA
BbsI 220 GAAGAC GAAGAC(2/6)
459 GAAGAC
BpuAI 220 GAAGAC GAAGAC(2/6)
459 GAAGAC
BseRI 496 GAGGAG GAGGAG(10/8)
885 GAGGAG
BsoBI 466 CCCGAG C^[CT]CG[GA]G
718 CCCGAG
Bsu36I 688 CCTAAGG CC^T[ACGT]AGG
850 CCTGAGG
EarI 756 GAAGAG CTCTTC(1/4)
772 GAAGAG
Eco81I 688 CCTAAGG CC^T[ACGT]AGG
850 CCTGAGG
EcoT14I 282 CCATGG C^C[AT][AT]GG
809 CCAAGG
HgaI 612 GACGC GACGC(5/10)
931 GACGC
HphI 527 TCACC GGTGA(8/7)
866 TCACC
Ksp632I 756 GAAGAG CTCTTC(1/4)
772 GAAGAG
MaeII 18 ACGT A^CGT
624 ACGT
MluNI 87 TGGCCA TGG^CCA
746 TGGCCA
MscI 87 TGGCCA TGG^CCA
746 TGGCCA
MseI 167 TTAA T^TAA
251 TTAA
MwoI 820 GCTCAGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
925 GCAGAGGACGC
NlaIII 283 CATG CATG^
838 CATG
PleI 197 GACTC GAGTC(4/5)
462 GACTC
SapI 756 GAAGAGC GCTCTTC(1/4)
772 GAAGAGC
StyI 282 CCATGG C^C[AT][AT]GG
809 CCAAGG
Tru9I 167 TTAA T^TAA
251 TTAA
TspRI 57 CACTG CA[GC]TG[ACGT][ACGT]^
563 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI BbvI BfaI
BpmI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI MaeI
MboI MboII MflI MnlI MvaI
NdeII NlaIV Sau3AI Sau96I ScrFI
SfaNI TfiI TseI XhoII