Restriction map for
pFN21AE2203
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HpaI KasI
KpnI KspI MaeI MamI MfeI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1040 GTCGAC GT^[AC][GT]AC
AflIII 1524 ACGCGT A^C[GA][CT]GT
Alw44I 585 GTGCAC G^TGCAC
AlwNI 979 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1177 CCTGAGG CC^T[ACGT]AGG
ApaLI 585 GTGCAC G^TGCAC
BalI 2098 TGGCCA TGG^CCA
BanI 1518 GGCACC G^G[CT][GA]CC
BbvCI 2131 GCTGAGG CCTCAGC(-5/-2)
BcgI 991 CGAGTGTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2562 GGATAC GTATCC(6/5)
BsaI 517 GGTCTC GGTCTC(1/5)
BsgI 1279 GTGCAG GTGCAG(16/14)
BsiEI 1145 CGGCCG CG[GA][CT]^CG
BsmBI 1416 CGTCTC CGTCTC(1/5)
BsmI 708 GAATGC GAATGC(1/-1)
BsrBI 332 GAGCGG CCGCTC(-3/-3)
BsrDI 402 GCAATG GCAATG(2/0)
BssSI 580 CTCGTG CACGAG(-5/-1)
Bsu36I 1177 CCTGAGG CC^T[ACGT]AGG
CfoI 147 GCGC GCG^C
DraII 782 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 2510 GACCAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1035 CCATGG C^C[GA][CT]GG
EagI 1145 CGGCCG C^GGCCG
EclXI 1145 CGGCCG C^GGCCG
Eco52I 1145 CGGCCG C^GGCCG
Eco81I 1177 CCTGAGG CC^T[ACGT]AGG
EcoO109I 782 AGGACCT [GA]G^G[ACGT]CC[CT]
HhaI 147 GCGC GCG^C
HinP1I 147 GCGC G^CGC
HincII 1040 GTCGAC GT[CT]^[GA]AC
HindII 1040 GTCGAC GT[CT]^[GA]AC
HindIII 1241 AAGCTT A^AGCTT
MluI 1524 ACGCGT A^CGCGT
MluNI 2098 TGGCCA TGG^CCA
MscI 2098 TGGCCA TGG^CCA
MslI 610 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 512 CAGCTG C[AC]G^C[GT]G
NaeI 1001 GCCGGC GCC^GGC
NcoI 1035 CCATGG C^CATGG
NgoMIV 1001 GCCGGC G^CCGGC
PpuMI 782 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 512 CAGCTG CAG^CTG
SacI 1400 GAGCTC GAGCT^C
SalI 1040 GTCGAC G^TCGAC
ScaI 1101 AGTACT AGT^ACT
SexAI 591 ACCAGGT A^CC[AT]GGT
Tsp45I 274 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 148 CGCG CG^CG
1525 CGCG
Asp700I 2179 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2320 GAAGCCTTTC
BbsI 1756 GAAGAC GAAGAC(2/6)
2500 GAAGAC
BpuAI 1756 GAAGAC GAAGAC(2/6)
2500 GAAGAC
BseRI 1882 GAGGAG GAGGAG(10/8)
2481 GAGGAG
BsiYI 22 CCGGGTTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1148 CCGTGAATTGG
BslI 22 CCGGGTTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1148 CCGTGAATTGG
BspHI 501 TCATGA T^CATGA
828 TCATGA
BspMI 138 GCAGGT ACCTGC(4/8)
1207 GCAGGT
BsrFI 1001 GCCGGC [GA]^CCGG[CT]
2566 ACCGGC
BstUI 148 CGCG CG^CG
1525 CGCG
Cac8I 790 GCACGC GC[ACGT]^[ACGT]GC
1001 GCCGGC
Cfr10I 1001 GCCGGC [GA]^CCGG[CT]
2566 ACCGGC
DraI 1389 TTTAAA TTT^AAA
2044 TTTAAA
EcoT14I 1035 CCATGG C^C[AT][AT]GG
1603 CCAAGG
MvnI 148 CGCG CG^CG
1525 CGCG
PleI 357 GACTC GAGTC(4/5)
1694 GACTC
PstI 889 CTGCAG CTGCA^G
1097 CTGCAG
RcaI 501 TCATGA T^CATGA
828 TCATGA
SapI 942 GAAGAGC GCTCTTC(1/4)
2590 GAAGAGC
SfcI 889 CTGCAG C^T[GA][CT]AG
1097 CTGCAG
StyI 1035 CCATGG C^C[AT][AT]GG
1603 CCAAGG
XcmI 1559 CCACCAAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1622 CCAATGACGAAGTGG
XmnI 2179 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2320 GAAGCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII