Restriction map for
pFN21AE5726
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlpI
Bpu1102I BsaI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI Ksp632I
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 55 TCCGGA T^CCGGA
AcsI 920 AAATTT [GA]^AATT[CT]
AcyI 138 GGCGTC G[GA]^CG[CT]C
AflIII 1489 ACATGT A^C[GA][CT]GT
AlwNI 432 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 332 GGGCCC GGGCC^C
ApoI 920 AAATTT [GA]^AATT[CT]
Asp700I 1161 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 329 CCCGGG C^[CT]CG[GA]G
AvrII 1683 CCTAGG C^CTAGG
BalI 1989 TGGCCA TGG^CCA
BlnI 1683 CCTAGG C^CTAGG
BsaAI 709 CACGTA [CT]AC^GT[GA]
BsaBI 1302 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 138 GGCGTC G[GA]^CG[CT]C
BsaWI 55 TCCGGA [AT]^CCGG[AT]
BseAI 55 TCCGGA T^CCGGA
BsmBI 147 GAGACG CGTCTC(1/5)
BsoBI 329 CCCGGG C^[CT]CG[GA]G
BspEI 55 TCCGGA T^CCGGA
BspLU11I 1489 ACATGT A^CATGT
BsrBI 336 CCGCTC CCGCTC(-3/-3)
BstAPI 1709 GCAATAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 502 GGTGACC G^GT[ACGT]ACC
BstPI 502 GGTGACC G^GT[ACGT]ACC
DraI 601 TTTAAA TTT^AAA
EaeI 1989 TGGCCA [CT]^GGCC[GA]
EcoO65I 502 GGTGACC G^GT[ACGT]ACC
EcoT22I 699 ATGCAT ATGCA^T
HgaI 139 GCGTC GACGC(5/10)
Hin1I 138 GGCGTC G[GA]^CG[CT]C
KspI 424 CCGCGG CCGC^GG
MamI 1302 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1989 TGGCCA TGG^CCA
MroI 55 TCCGGA T^CCGGA
MscI 1989 TGGCCA TGG^CCA
MslI 790 CACAAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1971 CCATGG C^CATGG
NdeI 724 CATATG CA^TATG
NsiI 699 ATGCAT ATGCA^T
PflMI 431 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 2034 CAGCTG CAG^CTG
SacII 424 CCGCGG CCGC^GG
SmaI 329 CCCGGG CCC^GGG
SmlI 1786 CTCAAG C^T[CT][GA]AG
SspI 1368 AATATT AAT^ATT
Van91I 431 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2121 TCTAGA T^CTAGA
XcmI 1208 CCAGCTTGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 329 CCCGGG C^CCGGG
XmaI 329 CCCGGG C^CCGGG
XmnI 1161 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AocI 79 CCTGAGG CC^T[ACGT]AGG
630 CCTGAGG
AseI 1825 ATTAAT AT^TAAT
1963 ATTAAT
AsnI 1825 ATTAAT AT^TAAT
1963 ATTAAT
AspHI 247 GTGCTC G[AT]GC[AT]^C
1678 GAGCAC
BsiHKAI 247 GTGCTC G[AT]GC[AT]^C
1678 GAGCAC
Bsu36I 79 CCTGAGG CC^T[ACGT]AGG
630 CCTGAGG
DsaI 424 CCGCGG C^C[GA][CT]GG
1971 CCATGG
Eco81I 79 CCTGAGG CC^T[ACGT]AGG
630 CCTGAGG
PpuMI 570 GGGTCCC [GA]G^G[AT]CC[CT]
664 GGGACCC
PshBI 1825 ATTAAT AT^TAAT
1963 ATTAAT
StuI 475 AGGCCT AGG^CCT
1080 AGGCCT
TfiI 1576 GATTC G^A[AT]TC
1634 GAATC
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AvaII BamHI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII