Restriction map for
pFN21AE3502
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccII AclI AflII Aor51HI
ApaI AscI AseI AsnI AspI
BalI BbrPI BfrI BsaAI BsiEI
BsiWI BsmI BsrBI BssHII BstEII
BstPI BstUI CpoI EaeI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
FseI KspI MaeII MluI MluNI
MscI MvnI NaeI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 2650 GTATAC GT^[AC][GT]AC
AccIII 2699 TCCGGA T^CCGGA
AgeI 4157 ACCGGT A^CCGGT
AviII 4673 TGCGCA TGC^GCA
AvrII 1462 CCTAGG C^CTAGG
BamHI 57 GGATCC G^GATCC
BbvCI 2422 CCTCAGC CCTCAGC(-5/-2)
BcgI 397 GCAGCAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1173 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1462 CCTAGG C^CTAGG
BseAI 2699 TCCGGA T^CCGGA
BsmBI 3284 CGTCTC CGTCTC(1/5)
Bsp1407I 4522 TGTACA T^GTACA
BspDI 4306 ATCGAT AT^CGAT
BspEI 2699 TCCGGA T^CCGGA
BsrDI 2852 GCAATG GCAATG(2/0)
BsrFI 4157 ACCGGT [GA]^CCGG[CT]
BsrGI 4522 TGTACA T^GTACA
BssSI 1102 CACGAG CACGAG(-5/-1)
Bst1107I 2650 GTATAC GTA^TAC
BstAPI 1127 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3197 TTCGAA TT^CGAA
BstZ17I 2650 GTATAC GTA^TAC
Cfr10I 4157 ACCGGT [GA]^CCGG[CT]
ClaI 4306 ATCGAT AT^CGAT
DraIII 1786 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 25 GACAGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3081 CCATGG C^C[GA][CT]GG
FspI 4673 TGCGCA TGC^GCA
HindIII 2140 AAGCTT A^AGCTT
MroI 2699 TCCGGA T^CCGGA
NcoI 3081 CCATGG C^CATGG
NspV 3197 TTCGAA TT^CGAA
PinAI 4157 ACCGGT A^CCGGT
PshAI 3278 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 296 AGTACT AGT^ACT
SfuI 3197 TTCGAA TT^CGAA
SmaI 4640 CCCGGG CCC^GGG
SmlI 4198 CTCAAG C^T[CT][GA]AG
SspBI 4522 TGTACA T^GTACA
SspI 69 AATATT AAT^ATT
StuI 3332 AGGCCT AGG^CCT
XcmI 2607 CCATGTTCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4640 CCCGGG C^CCGGG
XmaI 4640 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1545 GGTACC G^GTACC
1575 GGTACC
AcsI 888 AAATTT [GA]^AATT[CT]
1555 GAATTT
AcyI 2177 GGCGCC G[GA]^CG[CT]C
2958 GGCGCC
AhdI 3255 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4093 GACTACATGTC
Alw44I 1271 GTGCAC G^TGCAC
4635 GTGCAC
ApaLI 1271 GTGCAC G^TGCAC
4635 GTGCAC
ApoI 888 AAATTT [GA]^AATT[CT]
1555 GAATTT
Asp718I 1545 GGTACC G^GTACC
1575 GGTACC
AspEI 3255 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4093 GACTACATGTC
AvaI 1328 CTCGGG C^[CT]CG[GA]G
4640 CCCGGG
BbeI 2177 GGCGCC GGCGC^C
2958 GGCGCC
BciVI 1812 GGATAC GTATCC(6/5)
1489 GTATCC
BglII 2359 AGATCT A^GATCT
3632 AGATCT
BlpI 491 GCTTAGC GC^T[ACGT]AGC
4283 GCTCAGC
BpmI 18 CTGGAG CTGGAG(16/14)
1648 CTGGAG
Bpu1102I 491 GCTTAGC GC^T[ACGT]AGC
4283 GCTCAGC
BsaBI 3786 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4291 GATGGATATC
BsaHI 2177 GGCGCC G[GA]^CG[CT]C
2958 GGCGCC
BsaWI 2699 TCCGGA [AT]^CCGG[AT]
4157 ACCGGT
BseRI 764 CTCCTC GAGGAG(10/8)
3310 GAGGAG
BsoBI 1328 CTCGGG C^[CT]CG[GA]G
4640 CCCGGG
BstXI 740 CCATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4580 CCAGGGTTCTGG
CelII 491 GCTTAGC GC^T[ACGT]AGC
4283 GCTCAGC
DraI 444 TTTAAA TTT^AAA
2623 TTTAAA
Eam1105I 3255 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4093 GACTACATGTC
EcoRV 2197 GATATC GAT^ATC
4295 GATATC
EcoT22I 4016 ATGCAT ATGCA^T
4062 ATGCAT
HaeII 2177 GGCGCC [GA]GCGC^[CT]
2958 GGCGCC
HgaI 692 GACGC GACGC(5/10)
3283 GCGTC
Hin1I 2177 GGCGCC G[GA]^CG[CT]C
2958 GGCGCC
KasI 2177 GGCGCC G^GCGCC
2958 GGCGCC
KpnI 1545 GGTACC GGTAC^C
1575 GGTACC
MamI 3786 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4291 GATGGATATC
MfeI 1895 CAATTG C^AATTG
3986 CAATTG
MunI 1895 CAATTG C^AATTG
3986 CAATTG
NarI 2177 GGCGCC GG^CGCC
2958 GGCGCC
NsiI 4016 ATGCAT ATGCA^T
4062 ATGCAT
PstI 1255 CTGCAG CTGCA^G
1471 CTGCAG
PvuII 152 CAGCTG CAG^CTG
4142 CAGCTG
SacI 4177 GAGCTC GAGCT^C
4723 GAGCTC
SapI 1195 GAAGAGC GCTCTTC(1/4)
4720 GAAGAGC
SfoI 2177 GGCGCC GGC^GCC
2958 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BclI
BcnI BfaI BmyI BpuAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
RcaI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI