Restriction map for
pFN21AE5842
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BlnI BsgI BsiEI
BsiWI BspDI BspHI BssHII BssSI
BstEII BstPI ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoT22I FseI HpaI KpnI KspI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SpeI Sse8387I StuI
SwaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 4764 GTATAC GT^[AC][GT]AC
AccII 4291 CGCG CG^CG
AcyI 4127 GGCGCC G[GA]^CG[CT]C
Alw44I 1877 GTGCAC G^TGCAC
Aor51HI 5510 AGCGCT AGC^GCT
ApaLI 1877 GTGCAC G^TGCAC
BbeI 4127 GGCGCC GGCGC^C
BbrPI 3353 CACGTG CAC^GTG
BcgI 1839 GCAGTTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2471 GGATAC GTATCC(6/5)
BglI 1295 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 4127 GGCGCC G[GA]^CG[CT]C
BsaI 4651 GAGACC GGTCTC(1/5)
BsmBI 5351 GAGACG CGTCTC(1/5)
Bsp1407I 5109 TGTACA T^GTACA
BsrBI 3373 GAGCGG CCGCTC(-3/-3)
BsrFI 4124 ACCGGC [GA]^CCGG[CT]
BsrGI 5109 TGTACA T^GTACA
Bst1107I 4764 GTATAC GTA^TAC
BstAPI 3796 GCATGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3617 TTCGAA TT^CGAA
BstUI 4291 CGCG CG^CG
BstZ17I 4764 GTATAC GTA^TAC
Cfr10I 4124 ACCGGC [GA]^CCGG[CT]
DraIII 2868 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 5510 AGCGCT AGC^GCT
EcoRV 1237 GATATC GAT^ATC
Hin1I 4127 GGCGCC G[GA]^CG[CT]C
HincII 3096 GTCAAC GT[CT]^[GA]AC
HindII 3096 GTCAAC GT[CT]^[GA]AC
KasI 4127 GGCGCC G^GCGCC
MfeI 2212 CAATTG C^AATTG
MunI 2212 CAATTG C^AATTG
MvnI 4291 CGCG CG^CG
NarI 4127 GGCGCC GG^CGCC
NspV 3617 TTCGAA TT^CGAA
PaeR7I 4976 CTCGAG C^TCGAG
PmaCI 3353 CACGTG CAC^GTG
PmlI 3353 CACGTG CAC^GTG
PshAI 2980 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2678 CTGCAG CTGCA^G
ScaI 1865 AGTACT AGT^ACT
SfoI 4127 GGCGCC GGC^GCC
SfuI 3617 TTCGAA TT^CGAA
SgrAI 4123 CACCGGCG C[GA]^CCGG[CT]G
SmaI 486 CCCGGG CCC^GGG
SphI 5288 GCATGC GCATG^C
SspBI 5109 TGTACA T^GTACA
SspI 1759 AATATT AAT^ATT
XbaI 1135 TCTAGA T^CTAGA
XhoI 4976 CTCGAG C^TCGAG
XmaCI 486 CCCGGG C^CCGGG
XmaI 486 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 2525 TCCGGA T^CCGGA
3421 TCCGGA
AflIII 1664 ACATGT A^C[GA][CT]GT
3317 ACATGT
Asp700I 682 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3610 GAATTCATTC
AspI 3485 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4149 GACGCTGTC
BbvCI 1282 CCTCAGC CCTCAGC(-5/-2)
4930 CCTCAGC
BclI 230 TGATCA T^GATCA
2288 TGATCA
BglII 4191 AGATCT A^GATCT
4403 AGATCT
BsaBI 2023 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5077 GATGGTGATC
BsaWI 2525 TCCGGA [AT]^CCGG[AT]
3421 TCCGGA
BseAI 2525 TCCGGA T^CCGGA
3421 TCCGGA
BspEI 2525 TCCGGA T^CCGGA
3421 TCCGGA
BspLU11I 1664 ACATGT A^CATGT
3317 ACATGT
BspMI 2076 GCAGGT ACCTGC(4/8)
5049 GCAGGT
BsrDI 2814 CATTGC GCAATG(2/0)
5583 CATTGC
EcoNI 5133 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5544 CCTGTCAGAGG
EcoRI 1616 GAATTC G^AATTC
3610 GAATTC
FbaI 230 TGATCA T^GATCA
2288 TGATCA
HaeII 4127 GGCGCC [GA]GCGC^[CT]
5510 AGCGCT
MamI 2023 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5077 GATGGTGATC
MroI 2525 TCCGGA T^CCGGA
3421 TCCGGA
NcoI 1690 CCATGG C^CATGG
5370 CCATGG
PflFI 3485 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4149 GACGCTGTC
SapI 814 GAAGAGC GCTCTTC(1/4)
5503 GAAGAGC
SnaBI 3020 TACGTA TAC^GTA
4658 TACGTA
Tth111I 3485 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4149 GACGCTGTC
XmnI 682 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3610 GAATTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII AviII BalI
BanI BanII BbsI BbvI BcnI
BfaI BfrI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII