Restriction map for
pFN21AE3830
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# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccII AcyI AgeI
Alw44I ApaI ApaLI AscI Asp718I
AviII BbeI BbrPI BsaBI BsaHI
BsiEI BsiWI BspDI BsrBI BssHII
BssSI BstUI BstXI ClaI DraIII
DrdI EagI EclXI Eco52I EcoRI
FseI FspI HgaI Hin1I KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PvuI SacI SacII SbfI SexAI
SfiI SfoI SgrAI SmaI Sse8387I
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 1587 TCCGGA T^CCGGA
AflII 1306 CTTAAG C^TTAAG
AflIII 4610 ACATGT A^C[GA][CT]GT
AhdI 2452 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 8722 CCTGAGG CC^T[ACGT]AGG
AspEI 2452 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5470 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6507 CCTAGG C^CTAGG
BfrI 1306 CTTAAG C^TTAAG
BlnI 6507 CCTAGG C^CTAGG
BsaAI 8280 TACGTA [CT]AC^GT[GA]
BseAI 1587 TCCGGA T^CCGGA
BsmBI 5636 GAGACG CGTCTC(1/5)
BspEI 1587 TCCGGA T^CCGGA
BspLU11I 4610 ACATGT A^CATGT
BsrFI 2183 GCCGGT [GA]^CCGG[CT]
Bsu36I 8722 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2183 GCCGGT [GA]^CCGG[CT]
CpoI 8033 CGGTCCG CG^G[AT]CCG
Eam1105I 2452 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 8722 CCTGAGG CC^T[ACGT]AGG
EcoRV 6148 GATATC GAT^ATC
FauI 7313 CCCGC CCCGC(4/6)
HpaI 2008 GTTAAC GTT^AAC
MroI 1587 TCCGGA T^CCGGA
PflFI 5470 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 7692 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 8033 CGGTCCG CG^G[AT]CCG
SnaBI 8280 TACGTA TAC^GTA
SphI 4281 GCATGC GCATG^C
Tth111I 5470 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6423 TCTAGA T^CTAGA
XcmI 1827 CCACTTGAACGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AclI 1834 AACGTT AA^CGTT
2230 AACGTT
AlwNI 2057 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
7697 CAGTCTCTG
Aor51HI 4544 AGCGCT AGC^GCT
8519 AGCGCT
AvaI 3935 CCCGAG C^[CT]CG[GA]G
7304 CTCGGG
BanI 4451 GGCACC G^G[CT][GA]CC
8214 GGTGCC
BbvCI 3777 CCTCAGC CCTCAGC(-5/-2)
6439 GCTGAGG
BcgI 4014 GCACTACATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
7242 GCAGACAGCTCG
BciVI 6395 GGATAC GTATCC(6/5)
4209 GTATCC
BglI 2676 GCCTAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6365 GCCCATGTGGC
BlpI 999 GCTAAGC GC^T[ACGT]AGC
3720 GCTAAGC
Bpu1102I 999 GCTAAGC GC^T[ACGT]AGC
3720 GCTAAGC
BsoBI 3935 CCCGAG C^[CT]CG[GA]G
7304 CTCGGG
Bsp1407I 434 TGTACA T^GTACA
8324 TGTACA
BsrGI 434 TGTACA T^GTACA
8324 TGTACA
Bst1107I 6051 GTATAC GTA^TAC
6700 GTATAC
BstAPI 2056 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5904 GCAGCCTGTGC
BstEII 2693 GGTCACC G^GT[ACGT]ACC
8140 GGTCACC
BstPI 2693 GGTCACC G^GT[ACGT]ACC
8140 GGTCACC
BstZ17I 6051 GTATAC GTA^TAC
6700 GTATAC
CelII 999 GCTAAGC GC^T[ACGT]AGC
3720 GCTAAGC
DraII 140 AGGACCT [GA]G^G[ACGT]CC[CT]
8254 GGGACCT
DsaI 5186 CCATGG C^C[GA][CT]GG
6890 CCATGG
Eco47III 4544 AGCGCT AGC^GCT
8519 AGCGCT
EcoO109I 140 AGGACCT [GA]G^G[ACGT]CC[CT]
8254 GGGACCT
EcoO65I 2693 GGTCACC G^GT[ACGT]ACC
8140 GGTCACC
EcoT22I 1497 ATGCAT ATGCA^T
4279 ATGCAT
NcoI 5186 CCATGG C^CATGG
6890 CCATGG
NsiI 1497 ATGCAT ATGCA^T
4279 ATGCAT
PflMI 4138 CCATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6885 CCAGCCCATGG
PpuMI 140 AGGACCT [GA]G^G[AT]CC[CT]
8254 GGGACCT
Psp1406I 1834 AACGTT AA^CGTT
2230 AACGTT
SalI 323 GTCGAC G^TCGAC
5552 GTCGAC
SspBI 434 TGTACA T^GTACA
8324 TGTACA
SspI 7144 AATATT AAT^ATT
7291 AATATT
Van91I 4138 CCATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6885 CCAGCCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BalI BamHI BanII
BbsI BbvI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstBI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EaeI EarI EcoNI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI NspV PleI PshBI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SfuI SmlI SpeI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI