Restriction map for
pFN21AB5875
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI EcoT22I FbaI FseI FspI
HindIII KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NdeI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SfiI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AclI 667 AACGTT AA^CGTT
AcsI 1238 AAATTC [GA]^AATT[CT]
AcyI 229 GGCGCC G[GA]^CG[CT]C
Aor51HI 351 AGCGCT AGC^GCT
ApaI 1030 GGGCCC GGGCC^C
ApoI 1238 AAATTC [GA]^AATT[CT]
Asp700I 1184 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 793 GAGCTC G[AT]GC[AT]^C
BanI 229 GGCGCC G^G[CT][GA]CC
BbeI 229 GGCGCC GGCGC^C
BbsI 1378 GAAGAC GAAGAC(2/6)
BpuAI 1378 GAAGAC GAAGAC(2/6)
BsaHI 229 GGCGCC G[GA]^CG[CT]C
BsiHKAI 793 GAGCTC G[AT]GC[AT]^C
BsmBI 542 CGTCTC CGTCTC(1/5)
BsmFI 357 GGGAC GGGAC(10/14)
BsrBI 20 CCGCTC CCGCTC(-3/-3)
BstBI 911 TTCGAA TT^CGAA
BstEII 39 GGTGACC G^GT[ACGT]ACC
BstPI 39 GGTGACC G^GT[ACGT]ACC
BstYI 176 AGATCC [GA]^GATC[CT]
DraI 447 TTTAAA TTT^AAA
DraII 1029 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 95 CCATGG C^C[GA][CT]GG
Eco47III 351 AGCGCT AGC^GCT
EcoNI 1311 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1029 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 39 GGTGACC G^GT[ACGT]ACC
EcoRV 658 GATATC GAT^ATC
HgaI 359 GACGC GACGC(5/10)
Hin1I 229 GGCGCC G[GA]^CG[CT]C
HincII 858 GTTAAC GT[CT]^[GA]AC
HindII 858 GTTAAC GT[CT]^[GA]AC
HpaI 858 GTTAAC GTT^AAC
KasI 229 GGCGCC G^GCGCC
MflI 176 AGATCC [GA]^GATC[CT]
NarI 229 GGCGCC GG^CGCC
NcoI 95 CCATGG C^CATGG
NheI 114 GCTAGC G^CTAGC
NspI 752 GCATGC [GA]CATG^[CT]
NspV 911 TTCGAA TT^CGAA
PflMI 717 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 988 GACTC GAGTC(4/5)
Psp1406I 667 AACGTT AA^CGTT
SacI 793 GAGCTC GAGCT^C
SapI 470 GAAGAGC GCTCTTC(1/4)
SexAI 35 ACCTGGT A^CC[AT]GGT
SfoI 229 GGCGCC GGC^GCC
SfuI 911 TTCGAA TT^CGAA
SmaI 159 CCCGGG CCC^GGG
SphI 752 GCATGC GCATG^C
StuI 771 AGGCCT AGG^CCT
Van91I 717 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 176 AGATCC [GA]^GATC[CT]
XmaCI 159 CCCGGG C^CCGGG
XmaI 159 CCCGGG C^CCGGG
XmnI 1184 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 177 GATCC GGATC(4/5)
828 GATCC
AlwNI 31 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
574 CAGCTCCTG
AvaI 159 CCCGGG C^[CT]CG[GA]G
1312 CTCGGG
BanII 793 GAGCTC G[GA]GC[CT]^C
1030 GGGCCC
BsgI 476 CTGCAC GTGCAG(16/14)
837 CTGCAC
BsmAI 1060 GAGAC GTCTC(1/5)
543 GTCTC
BsoBI 159 CCCGGG C^[CT]CG[GA]G
1312 CTCGGG
BsrFI 241 GCCGGC [GA]^CCGG[CT]
283 GCCGGC
BstNI 36 CCTGG CC^[AT]GG
843 CCTGG
BstXI 665 CCAACGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1130 CCAAAGACTTGG
Cfr10I 241 GCCGGC [GA]^CCGG[CT]
283 GCCGGC
EcoRII 36 CCTGG ^CC[AT]GG
843 CCTGG
FauI 19 CCCGC CCCGC(4/6)
1123 CCCGC
HaeII 229 GGCGCC [GA]GCGC^[CT]
351 AGCGCT
MvaI 36 CCTGG CC^[AT]GG
843 CCTGG
NaeI 241 GCCGGC GCC^GGC
283 GCCGGC
NgoMIV 241 GCCGGC G^CCGGC
283 GCCGGC
TaqI 912 TCGA T^CGA
1343 TCGA
TthHB8I 912 TCGA T^CGA
1343 TCGA
XcmI 585 CCAGTCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1095 CCAAGACCACAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstUI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI