Restriction map for
pFN21AE2231
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BbrPI BcgI BciVI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoRV EcoT14I FauI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SspBI SspI StyI
SwaI XcmI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 691 CGCG CG^CG
AflII 982 CTTAAG C^TTAAG
AhdI 1079 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 735 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1079 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 757 GAGCTC G[AT]GC[AT]^C
AvaI 428 CCCGGG C^[CT]CG[GA]G
BamHI 1460 GGATCC G^GATCC
BbvCI 591 CCTCAGC CCTCAGC(-5/-2)
BclI 1443 TGATCA T^GATCA
BfrI 982 CTTAAG C^TTAAG
BsiHKAI 757 GAGCTC G[AT]GC[AT]^C
BsmAI 1508 GAGAC GTCTC(1/5)
BsoBI 428 CCCGGG C^[CT]CG[GA]G
BspHI 1398 TCATGA T^CATGA
BsrDI 1186 CATTGC GCAATG(2/0)
BstEII 703 GGTTACC G^GT[ACGT]ACC
BstPI 703 GGTTACC G^GT[ACGT]ACC
BstUI 691 CGCG CG^CG
BstXI 1380 CCAGTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 498 GCGC GCG^C
DrdI 674 GACTAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1079 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 703 GGTTACC G^GT[ACGT]ACC
EcoRI 847 GAATTC G^AATTC
EcoT22I 610 ATGCAT ATGCA^T
FbaI 1443 TGATCA T^GATCA
FseI 381 GGCCGGCC GGCCGG^CC
HaeII 497 GGCGCT [GA]GCGC^[CT]
HhaI 498 GCGC GCG^C
HinP1I 498 GCGC G^CGC
HindIII 226 AAGCTT A^AGCTT
MslI 1435 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 691 CGCG CG^CG
NaeI 382 GCCGGC GCC^GGC
NgoMIV 382 GCCGGC G^CCGGC
NsiI 610 ATGCAT ATGCA^T
PflMI 796 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1079 GACTC GAGTC(4/5)
PstI 552 CTGCAG CTGCA^G
RcaI 1398 TCATGA T^CATGA
SacI 757 GAGCTC GAGCT^C
SmaI 428 CCCGGG CCC^GGG
SpeI 346 ACTAGT A^CTAGT
Van91I 796 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 120 TCTAGA T^CTAGA
XmaCI 428 CCCGGG C^CCGGG
XmaI 428 CCCGGG C^CCGGG
XmnI 735 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 93 GAATTT [GA]^AATT[CT]
847 GAATTC
AfaI 818 GTAC GT^AC
979 GTAC
ApoI 93 GAATTT [GA]^AATT[CT]
847 GAATTC
AspI 355 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
674 GACTAAGTC
BalI 188 TGGCCA TGG^CCA
917 TGGCCA
BanI 835 GGCACC G^G[CT][GA]CC
1014 GGCACC
BanII 579 GAGCCC G[GA]GC[CT]^C
757 GAGCTC
BbsI 361 GTCTTC GAAGAC(2/6)
1087 GTCTTC
BpuAI 361 GTCTTC GAAGAC(2/6)
1087 GTCTTC
BsrFI 382 GCCGGC [GA]^CCGG[CT]
707 ACCGGC
BstYI 1048 AGATCC [GA]^GATC[CT]
1460 GGATCC
Cfr10I 382 GCCGGC [GA]^CCGG[CT]
707 ACCGGC
EcoNI 141 CCTTTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1032 CCTCTGTCAGG
HgaI 605 GACGC GACGC(5/10)
692 GCGTC
MflI 1048 AGATCC [GA]^GATC[CT]
1460 GGATCC
MluNI 188 TGGCCA TGG^CCA
917 TGGCCA
MscI 188 TGGCCA TGG^CCA
917 TGGCCA
MseI 170 TTAA T^TAA
983 TTAA
NspI 370 GCATGC [GA]CATG^[CT]
608 GCATGC
PflFI 355 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
674 GACTAAGTC
RsaI 818 GTAC GT^AC
979 GTAC
SapI 735 GAAGAGC GCTCTTC(1/4)
1251 GAAGAGC
SfcI 510 CTATAG C^T[GA][CT]AG
552 CTGCAG
SphI 370 GCATGC GCATG^C
608 GCATGC
TaqI 23 TCGA T^CGA
312 TCGA
Tru9I 170 TTAA T^TAA
983 TTAA
Tsp509I 94 AATT ^AATT
848 AATT
Tth111I 355 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
674 GACTAAGTC
TthHB8I 23 TCGA T^CGA
312 TCGA
XhoII 1048 AGATCC [GA]^GATC[CT]
1460 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PvuII Sau3AI Sau96I ScrFI SfaNI
SmlI StuI TfiI TseI Tsp45I
TspRI