################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbrPI BciVI BclI BfrI BglII BlnI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI MamI MfeI MluI MroI MslI MunI NaeI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SfiI SgrAI SmlI SnaBI SpeI SphI SspBI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 1375 GACGTC GACGT^C AccI 1699 GTCTAC GT^[AC][GT]AC AflIII 221 ACGTGT A^C[GA][CT]GT AgeI 143 ACCGGT A^CCGGT Asp700I 1152 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 1069 TGGCCA TGG^CCA BamHI 1748 GGATCC G^GATCC BbeI 1916 GGCGCC GGCGC^C BcgI 582 CGATCCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 707 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1860 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1860 GCTGAGC GC^T[ACGT]AGC BsaAI 1627 TACGTG [CT]AC^GT[GA] BsgI 736 GTGCAG GTGCAG(16/14) BsmBI 218 GAGACG CGTCTC(1/5) BstBI 192 TTCGAA TT^CGAA BstXI 1588 CCATCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1860 GCTGAGC GC^T[ACGT]AGC DraI 1179 TTTAAA TTT^AAA DrdI 1276 GACTACAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1069 TGGCCA [CT]^GGCC[GA] EcoNI 495 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 1916 GGCGCC [GA]GCGC^[CT] HindIII 1390 AAGCTT A^AGCTT KasI 1916 GGCGCC G^GCGCC KspI 213 CCGCGG CCGC^GG MluNI 1069 TGGCCA TGG^CCA MscI 1069 TGGCCA TGG^CCA NarI 1916 GGCGCC GG^CGCC NheI 469 GCTAGC G^CTAGC NspI 171 GCATGT [GA]CATG^[CT] NspV 192 TTCGAA TT^CGAA PinAI 143 ACCGGT A^CCGGT PpuMI 299 AGGACCC [GA]G^G[AT]CC[CT] PshAI 610 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1614 GAGCTC GAGCT^C SacII 213 CCGCGG CCGC^GG SapI 1903 GAAGAGC GCTCTTC(1/4) SbfI 1689 CCTGCAGG CCTGCA^GG SexAI 1673 ACCTGGT A^CC[AT]GGT SfaNI 859 GATGC GCATC(5/9) SfoI 1916 GGCGCC GGC^GCC SfuI 192 TTCGAA TT^CGAA Sse8387I 1689 CCTGCAGG CCTGCA^GG XmnI 1152 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### ApaI 790 GGGCCC GGGCC^C 831 GGGCCC AspHI 1614 GAGCTC G[AT]GC[AT]^C 1723 GTGCTC BbvCI 598 GCTGAGG CCTCAGC(-5/-2) 1831 GCTGAGG BfaI 470 CTAG C^TAG 1036 CTAG BsaI 1642 GAGACC GGTCTC(1/5) 146 GGTCTC BsiHKAI 1614 GAGCTC G[AT]GC[AT]^C 1723 GTGCTC BsmI 682 GAATGC GAATGC(1/-1) 1031 GAATGC BspMI 1559 ACCTGC ACCTGC(4/8) 1688 ACCTGC BsrBI 1482 CCGCTC CCGCTC(-3/-3) 1906 GAGCGG BsrFI 143 ACCGGT [GA]^CCGG[CT] 1913 ACCGGC BstYI 487 GGATCT [GA]^GATC[CT] 1748 GGATCC Cfr10I 143 ACCGGT [GA]^CCGG[CT] 1913 ACCGGC HphI 1680 GGTGA GGTGA(8/7) 1271 TCACC MaeI 470 CTAG C^TAG 1036 CTAG MaeIII 805 GTGAC ^GT[ACGT]AC 1630 GTGAC MflI 487 GGATCT [GA]^GATC[CT] 1748 GGATCC MseI 1172 TTAA T^TAA 1180 TTAA SmaI 775 CCCGGG CCC^GGG 1496 CCCGGG SspI 1325 AATATT AAT^ATT 1881 AATATT Tru9I 1172 TTAA T^TAA 1180 TTAA Tsp45I 805 GTGAC ^GT[GC]AC 1630 GTGAC Tsp509I 424 AATT ^AATT 940 AATT XcmI 390 CCAAGATGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1508 CCAACCAGAAGCTGG XhoII 487 GGATCT [GA]^GATC[CT] 1748 GGATCC XmaCI 775 CCCGGG C^CCGGG 1496 CCCGGG XmaI 775 CCCGGG C^CCGGG 1496 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TfiI TseI TspRI TthHB8I Van91I