Restriction map for
pFN21AE2670
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AcyI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstXI CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KspI MamI
MfeI MluNI MscI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 799 GGTACC G^GTACC
AccII 30 CGCG CG^CG
AccIII 110 TCCGGA T^CCGGA
AclI 467 AACGTT AA^CGTT
AcsI 523 AAATTC [GA]^AATT[CT]
AflIII 29 ACGCGT A^C[GA][CT]GT
Alw44I 21 GTGCAC G^TGCAC
AlwNI 2039 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 21 GTGCAC G^TGCAC
ApoI 523 AAATTC [GA]^AATT[CT]
Asp718I 799 GGTACC G^GTACC
AvaI 1463 CTCGAG C^[CT]CG[GA]G
BbsI 1989 GAAGAC GAAGAC(2/6)
BciVI 1429 GGATAC GTATCC(6/5)
BglII 2020 AGATCT A^GATCT
BpuAI 1989 GAAGAC GAAGAC(2/6)
BsaI 1677 GAGACC GGTCTC(1/5)
BsaWI 110 TCCGGA [AT]^CCGG[AT]
BseAI 110 TCCGGA T^CCGGA
BsoBI 1463 CTCGAG C^[CT]CG[GA]G
BspEI 110 TCCGGA T^CCGGA
BspMI 1283 ACCTGC ACCTGC(4/8)
Bst1107I 1233 GTATAC GTA^TAC
BstUI 30 CGCG CG^CG
BstZ17I 1233 GTATAC GTA^TAC
DsaI 1138 CCATGG C^C[GA][CT]GG
EcoNI 784 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 172 GATATC GAT^ATC
EcoT14I 1138 CCATGG C^C[AT][AT]GG
HgaI 28 GACGC GACGC(5/10)
HincII 550 GTCAAC GT[CT]^[GA]AC
HindII 550 GTCAAC GT[CT]^[GA]AC
KpnI 799 GGTACC GGTAC^C
MluI 29 ACGCGT A^CGCGT
MroI 110 TCCGGA T^CCGGA
MvnI 30 CGCG CG^CG
NcoI 1138 CCATGG C^CATGG
PaeR7I 1463 CTCGAG C^TCGAG
PflMI 1150 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 467 AACGTT AA^CGTT
SacI 955 GAGCTC GAGCT^C
StyI 1138 CCATGG C^C[AT][AT]GG
Van91I 1150 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1463 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 1178 GTCTAC GT^[AC][GT]AC
1233 GTATAC
AocI 396 CCTAAGG CC^T[ACGT]AGG
1938 CCTGAGG
BcnI 102 CCGGG CC^[GC]GG
168 CCCGG
BsgI 808 CTGCAC GTGCAG(16/14)
1050 GTGCAG
BsmI 840 GAATGC GAATGC(1/-1)
106 GCATTC
Bsu36I 396 CCTAAGG CC^T[ACGT]AGG
1938 CCTGAGG
DraII 1721 AGGACCC [GA]G^G[ACGT]CC[CT]
1875 GGGTCCT
EaeI 287 CGGCCA [CT]^GGCC[GA]
725 CGGCCA
EarI 533 CTCTTC CTCTTC(1/4)
1000 GAAGAG
Eco81I 396 CCTAAGG CC^T[ACGT]AGG
1938 CCTGAGG
EcoO109I 1721 AGGACCC [GA]G^G[ACGT]CC[CT]
1875 GGGTCCT
FauI 595 CCCGC CCCGC(4/6)
1681 CCCGC
Ksp632I 533 CTCTTC CTCTTC(1/4)
1000 GAAGAG
NciI 102 CCGGG CC^[GC]GG
168 CCCGG
PpuMI 1721 AGGACCC [GA]G^G[AT]CC[CT]
1875 GGGTCCT
PstI 990 CTGCAG CTGCA^G
1751 CTGCAG
Tsp509I 438 AATT ^AATT
524 AATT
XcmI 225 CCAGAGGAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1998 CCAGCTAGAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BamHI BanI BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII