Restriction map for
pFN21AE5258
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AflII AgeI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MflI MluNI MroI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsaI RsrII SacI
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI SwaI TaqI
Tsp509I TthHB8I XbaI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 679 GACGTC GACGT^C
AflIII 385 ACGCGT A^C[GA][CT]GT
AhdI 632 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 547 AGCT AG^CT
Alw44I 83 GTGCAC G^TGCAC
AlwI 743 GGATC GGATC(4/5)
ApaLI 83 GTGCAC G^TGCAC
AspEI 632 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 261 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 230 TGCGCA TGC^GCA
BanI 557 GGCGCC G^G[CT][GA]CC
BanII 465 GAGCCC G[GA]GC[CT]^C
BbeI 557 GGCGCC GGCGC^C
BfaI 334 CTAG C^TAG
BseRI 10 CTCCTC GAGGAG(10/8)
BsgI 581 GTGCAG GTGCAG(16/14)
BsmBI 266 CGTCTC CGTCTC(1/5)
BspHI 200 TCATGA T^CATGA
BspMI 227 ACCTGC ACCTGC(4/8)
BsrI 164 ACTGG ACTGG(1/-1)
BssHII 298 GCGCGC G^CGCGC
BstAPI 222 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 744 GATC ^GATC
DraIII 163 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 632 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 759 GAAGAG CTCTTC(1/4)
FspI 230 TGCGCA TGC^GCA
HgaI 128 GACGC GACGC(5/10)
KasI 557 GGCGCC G^GCGCC
Ksp632I 759 GAAGAG CTCTTC(1/4)
KspI 371 CCGCGG CCGC^GG
MaeI 334 CTAG C^TAG
MboI 744 GATC ^GATC
MboII 759 GAAGA GAAGA(8/7)
MluI 385 ACGCGT A^CGCGT
MseI 134 TTAA T^TAA
NarI 557 GGCGCC GG^CGCC
NdeII 744 GATC ^GATC
PflFI 261 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 729 CCACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 630 GGGACCC [GA]G^G[AT]CC[CT]
PstI 655 CTGCAG CTGCA^G
RcaI 200 TCATGA T^CATGA
SacII 371 CCGCGG CCGC^GG
Sau3AI 744 GATC ^GATC
SbfI 654 CCTGCAGG CCTGCA^GG
SfcI 655 CTGCAG C^T[GA][CT]AG
SfoI 557 GGCGCC GGC^GCC
SmaI 399 CCCGGG CCC^GGG
Sse8387I 654 CCTGCAGG CCTGCA^GG
StuI 407 AGGCCT AGG^CCT
TfiI 607 GAATC G^A[AT]TC
Tru9I 134 TTAA T^TAA
Tth111I 261 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 729 CCACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 441 CCACAACGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 399 CCCGGG C^CCGGG
XmaI 399 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 557 GGCGCC G[GA]^CG[CT]C
679 GACGTC
AspHI 83 GTGCAC G[AT]GC[AT]^C
220 GAGCAC
AvaI 269 CTCGGG C^[CT]CG[GA]G
399 CCCGGG
BsaHI 557 GGCGCC G[GA]^CG[CT]C
679 GACGTC
BsiHKAI 83 GTGCAC G[AT]GC[AT]^C
220 GAGCAC
BsmAI 267 GTCTC GTCTC(1/5)
485 GTCTC
BsoBI 269 CTCGGG C^[CT]CG[GA]G
399 CCCGGG
DraII 428 GGGGCCT [GA]G^G[ACGT]CC[CT]
630 GGGACCC
EcoO109I 428 GGGGCCT [GA]G^G[ACGT]CC[CT]
630 GGGACCC
EcoT14I 100 CCATGG C^C[AT][AT]GG
572 CCATGG
HaeII 142 GGCGCT [GA]GCGC^[CT]
557 GGCGCC
HapII 400 CCGG C^CGG
471 CCGG
Hin1I 557 GGCGCC G[GA]^CG[CT]C
679 GACGTC
HpaII 400 CCGG C^CGG
471 CCGG
MslI 201 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
570 CACCATGGTG
MspA1I 371 CCGCGG C[AC]G^C[GT]G
458 CCGCTG
MspI 400 CCGG C^CGG
471 CCGG
NcoI 100 CCATGG C^CATGG
572 CCATGG
PleI 622 GACTC GAGTC(4/5)
483 GAGTC
SfaNI 34 GCATC GCATC(5/9)
650 GCATC
StyI 100 CCATGG C^C[AT][AT]GG
572 CCATGG
TspRI 163 CACTG CA[GC]TG[ACGT][ACGT]^
184 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AvaII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DsaI EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeII
MaeIII MnlI MvaI MvnI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
TseI Tsp45I