Restriction map for
pFN21AB8529
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcgI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspHI BsrBI
BsrGI BssHII Bst1107I BstUI BstXI
BstZ17I CelII CfoI ClaI CpoI
DraII DraIII EaeI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI HinP1I KasI KpnI
KspI MaeII MamI MluI MluNI
MscI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SwaI XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 2142 GTCTAC GT^[AC][GT]AC
AccIII 1125 TCCGGA T^CCGGA
AciI 2300 CCGC CCGC(-3/-1)
AcyI 4 GACGCC G[GA]^CG[CT]C
AhdI 2134 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1306 ATTAAT AT^TAAT
AsnI 1306 ATTAAT AT^TAAT
AspEI 2134 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2354 GAGCTC G[AT]GC[AT]^C
AspI 2242 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 94 GGCACC G^G[CT][GA]CC
BanII 2354 GAGCTC G[GA]GC[CT]^C
BbvCI 1143 CCTCAGC CCTCAGC(-5/-2)
BglII 366 AGATCT A^GATCT
BsaHI 4 GACGCC G[GA]^CG[CT]C
BseAI 1125 TCCGGA T^CCGGA
BseRI 808 CTCCTC GAGGAG(10/8)
BsgI 481 GTGCAG GTGCAG(16/14)
BsiHKAI 2354 GAGCTC G[AT]GC[AT]^C
BsmFI 2132 GGGAC GGGAC(10/14)
BsmI 420 GAATGC GAATGC(1/-1)
BspEI 1125 TCCGGA T^CCGGA
BsrFI 1963 ACCGGC [GA]^CCGG[CT]
BssSI 2333 CTCGTG CACGAG(-5/-1)
BstAPI 615 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1253 TTCGAA TT^CGAA
Cfr10I 1963 ACCGGC [GA]^CCGG[CT]
DrdI 820 GACAGTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2128 CCATGG C^C[GA][CT]GG
Eam1105I 2134 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 4 GACGCC G[GA]^CG[CT]C
HpaI 963 GTTAAC GTT^AAC
MfeI 2192 CAATTG C^AATTG
MroI 1125 TCCGGA T^CCGGA
MslI 846 CATAACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1439 CAGCTG C[AC]G^C[GT]G
MunI 2192 CAATTG C^AATTG
NcoI 2128 CCATGG C^CATGG
NlaIV 94 GGCACC GG[ACGT]^[ACGT]CC
NspV 1253 TTCGAA TT^CGAA
PflFI 2242 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2242 GACTC GAGTC(4/5)
PshBI 1306 ATTAAT AT^TAAT
PvuII 1439 CAGCTG CAG^CTG
SacI 2354 GAGCTC GAGCT^C
SbfI 2269 CCTGCAGG CCTGCA^GG
SfuI 1253 TTCGAA TT^CGAA
Sse8387I 2269 CCTGCAGG CCTGCA^GG
SspI 773 AATATT AAT^ATT
StuI 2322 AGGCCT AGG^CCT
Tth111I 2242 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1118 ACATGT A^C[GA][CT]GT
2031 ACATGT
AlwNI 620 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2562 CAGAGACTG
AocI 1026 CCTCAGG CC^T[ACGT]AGG
1351 CCTCAGG
AvaII 1339 GGTCC G^G[AT]CC
2581 GGACC
BciVI 19 GGATAC GTATCC(6/5)
2513 GTATCC
BmyI 232 GTGCCC G[AGT]GC[ACT]^C
2354 GAGCTC
Bsp1286I 232 GTGCCC G[AGT]GC[ACT]^C
2354 GAGCTC
BspLU11I 1118 ACATGT A^CATGT
2031 ACATGT
BstEII 442 GGTGACC G^GT[ACGT]ACC
1803 GGTTACC
BstPI 442 GGTGACC G^GT[ACGT]ACC
1803 GGTTACC
BstYI 366 AGATCT [GA]^GATC[CT]
526 GGATCT
Bsu36I 1026 CCTCAGG CC^T[ACGT]AGG
1351 CCTCAGG
EarI 811 CTCTTC CTCTTC(1/4)
2360 GAAGAG
Eco81I 1026 CCTCAGG CC^T[ACGT]AGG
1351 CCTCAGG
EcoO65I 442 GGTGACC G^GT[ACGT]ACC
1803 GGTTACC
EcoT14I 2128 CCATGG C^C[AT][AT]GG
2702 CCTTGG
Ksp632I 811 CTCTTC CTCTTC(1/4)
2360 GAAGAG
MflI 366 AGATCT [GA]^GATC[CT]
526 GGATCT
NspI 1118 ACATGT [GA]CATG^[CT]
2031 ACATGT
SfaNI 2156 GATGC GCATC(5/9)
2371 GATGC
StyI 2128 CCATGG C^C[AT][AT]GG
2702 CCTTGG
XhoII 366 AGATCT [GA]^GATC[CT]
526 GGATCT
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbvI BfaI BpmI BsaJI
BsaWI BsiYI BslI BsmAI BspMI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EcoRI EcoRII Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HindIII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
PflMI PstI RsaI Sau3AI Sau96I
ScrFI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XmnI