Restriction map for
pFN21AE9542
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspI AvaI
AvrII BamHI BbeI BbrPI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI HaeII
HgaI Hin1I KasI KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1975 GGTACC G^GTACC
AclI 1883 AACGTT AA^CGTT
AflIII 1152 ACATGT A^C[GA][CT]GT
AhdI 65 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1975 GGTACC G^GTACC
AspEI 65 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 78 GTGCTC G[AT]GC[AT]^C
AviII 1425 TGCGCA TGC^GCA
BanII 1724 GAGCCC G[GA]GC[CT]^C
BbsI 16 GTCTTC GAAGAC(2/6)
BbvCI 124 CCTCAGC CCTCAGC(-5/-2)
BciVI 1922 GGATAC GTATCC(6/5)
BcnI 571 CCGGG CC^[GC]GG
BglII 585 AGATCT A^GATCT
BpuAI 16 GTCTTC GAAGAC(2/6)
BsgI 133 CTGCAC GTGCAG(16/14)
BsiHKAI 78 GTGCTC G[AT]GC[AT]^C
BspHI 447 TCATGA T^CATGA
BspLU11I 1152 ACATGT A^CATGT
BstAPI 2175 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1131 CCAGTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1426 GCGC GCG^C
DraI 2062 TTTAAA TTT^AAA
DraII 947 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 111 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 384 GACTGCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 65 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1694 CTCTTC CTCTTC(1/4)
EcoO109I 947 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1910 ATGCAT ATGCA^T
FauI 158 GCGGG CCCGC(4/6)
FspI 1425 TGCGCA TGC^GCA
HhaI 1426 GCGC GCG^C
HinP1I 1426 GCGC G^CGC
HindIII 418 AAGCTT A^AGCTT
HpaI 1506 GTTAAC GTT^AAC
KpnI 1975 GGTACC GGTAC^C
Ksp632I 1694 CTCTTC CTCTTC(1/4)
MfeI 967 CAATTG C^AATTG
MunI 967 CAATTG C^AATTG
NciI 571 CCGGG CC^[GC]GG
NdeI 2135 CATATG CA^TATG
NsiI 1910 ATGCAT ATGCA^T
PleI 2255 GACTC GAGTC(4/5)
PpuMI 947 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1883 AACGTT AA^CGTT
RcaI 447 TCATGA T^CATGA
SexAI 1971 ACCAGGT A^CC[AT]GGT
SmlI 1033 CTCAAG C^T[CT][GA]AG
TaqI 633 TCGA T^CGA
TthHB8I 633 TCGA T^CGA
XbaI 1194 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AlwI 1872 GATCC GGATC(4/5)
1465 GGATC
AseI 847 ATTAAT AT^TAAT
1759 ATTAAT
AsnI 847 ATTAAT AT^TAAT
1759 ATTAAT
BalI 1472 TGGCCA TGG^CCA
2167 TGGCCA
BanI 1672 GGCACC G^G[CT][GA]CC
1975 GGTACC
BbvI 1160 GCAGC GCAGC(8/12)
1428 GCAGC
BmyI 78 GTGCTC G[AGT]GC[ACT]^C
1724 GAGCCC
BpmI 880 CTCCAG CTGGAG(16/14)
325 CTGGAG
BsaI 1563 GAGACC GGTCTC(1/5)
203 GGTCTC
BsaJI 84 CCTTGG C^C[ACGT][ACGT]GG
318 CCAAGG
Bsp1286I 78 GTGCTC G[AGT]GC[ACT]^C
1724 GAGCCC
BspMI 467 ACCTGC ACCTGC(4/8)
1255 ACCTGC
BsrDI 306 GCAATG GCAATG(2/0)
388 GCAATG
BstNI 1567 CCTGG CC^[AT]GG
1972 CCAGG
BstYI 585 AGATCT [GA]^GATC[CT]
1465 GGATCT
EcoRII 1567 CCTGG ^CC[AT]GG
1972 CCAGG
EcoT14I 84 CCTTGG C^C[AT][AT]GG
318 CCAAGG
HapII 571 CCGG C^CGG
2203 CCGG
HincII 728 GTCAAC GT[CT]^[GA]AC
1506 GTTAAC
HindII 728 GTCAAC GT[CT]^[GA]AC
1506 GTTAAC
HpaII 571 CCGG C^CGG
2203 CCGG
MaeII 695 ACGT A^CGT
1884 ACGT
MflI 585 AGATCT [GA]^GATC[CT]
1465 GGATCT
MluNI 1472 TGGCCA TGG^CCA
2167 TGGCCA
MscI 1472 TGGCCA TGG^CCA
2167 TGGCCA
MspA1I 1161 CAGCTG C[AC]G^C[GT]G
1429 CAGCTG
MspI 571 CCGG C^CGG
2203 CCGG
MvaI 1567 CCTGG CC^[AT]GG
1972 CCAGG
PflMI 318 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
882 CCAGAAACTGG
PshBI 847 ATTAAT AT^TAAT
1759 ATTAAT
PvuII 1161 CAGCTG CAG^CTG
1429 CAGCTG
ScaI 1133 AGTACT AGT^ACT
1444 AGTACT
SfaNI 1909 GATGC GCATC(5/9)
2181 GATGC
SphI 1283 GCATGC GCATG^C
1422 GCATGC
SspI 44 AATATT AAT^ATT
221 AATATT
StyI 84 CCTTGG C^C[AT][AT]GG
318 CCAAGG
TseI 1160 GCAGC G^C[AT]GC
1428 GCAGC
Van91I 318 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
882 CCAGAAACTGG
XcmI 739 CCAAGACTACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2143 CCATCATTCATCTGG
XhoII 585 AGATCT [GA]^GATC[CT]
1465 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I AvaII BfaI BsiYI
BslI BsmAI BsrI BssKI BstF5I
Cac8I Cfr13I DdeI DpnII EaeI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MwoI NdeII
NlaIII NlaIV NspI PstI RsaI
Sau3AI Sau96I ScrFI SfcI TfiI
Tru9I Tsp45I Tsp509I TspRI XmnI