Restriction map for
pFN21AE2485
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AocI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BseAI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I Eco81I EcoNI EcoO65I
EcoRV FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MroI MseI MunI
NarI NdeI NheI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI ScaI
SfoI SfuI SmaI SnaBI SpeI
SspBI SspI SwaI Tru9I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 1959 GAATTC [GA]^AATT[CT]
AflIII 590 ACGCGT A^C[GA][CT]GT
Alw44I 1480 GTGCAC G^TGCAC
Aor51HI 1766 AGCGCT AGC^GCT
ApaLI 1480 GTGCAC G^TGCAC
ApoI 1959 GAATTC [GA]^AATT[CT]
AspI 1500 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfaI 437 CTAG C^TAG
BlpI 1769 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1769 GCTCAGC GC^T[ACGT]AGC
BsaI 497 GAGACC GGTCTC(1/5)
BsaWI 1818 TCCGGT [AT]^CCGG[AT]
BsiWI 587 CGTACG C^GTACG
BsmI 1756 GAATGC GAATGC(1/-1)
BspMI 1734 GCAGGT ACCTGC(4/8)
BssHII 93 GCGCGC G^CGCGC
BstXI 1541 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1769 GCTCAGC GC^T[ACGT]AGC
DraIII 409 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1870 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2043 GAAGAG CTCTTC(1/4)
Eco47III 1766 AGCGCT AGC^GCT
EcoRI 1959 GAATTC G^AATTC
EcoT22I 140 ATGCAT ATGCA^T
Ksp632I 2043 GAAGAG CTCTTC(1/4)
MaeI 437 CTAG C^TAG
MluI 590 ACGCGT A^CGCGT
MslI 1881 CATTTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 610 CCATGG C^CATGG
NotI 1715 GCGGCCGC GC^GGCCGC
NsiI 140 ATGCAT ATGCA^T
NspI 1577 GCATGC [GA]CATG^[CT]
PflFI 1500 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 773 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1475 ACCAGGT A^CC[AT]GGT
SfiI 930 GGCCATCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1042 CTCAAG C^T[CT][GA]AG
SphI 1577 GCATGC GCATG^C
Tsp509I 1960 AATT ^AATT
Tth111I 1500 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 427 GACGTC GACGT^C
603 GACGTC
AlwNI 1153 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1777 CAGGAGCTG
AspHI 1480 GTGCAC G[AT]GC[AT]^C
1803 GAGCAC
AvaI 258 CTCGGG C^[CT]CG[GA]G
649 CCCGAG
BalI 1851 TGGCCA TGG^CCA
1905 TGGCCA
BsiHKAI 1480 GTGCAC G[AT]GC[AT]^C
1803 GAGCAC
BsmBI 1327 GAGACG CGTCTC(1/5)
1648 GAGACG
BsoBI 258 CTCGGG C^[CT]CG[GA]G
649 CCCGAG
BsrBI 169 CCGCTC CCGCTC(-3/-3)
762 CCGCTC
BstYI 1553 AGATCC [GA]^GATC[CT]
1976 AGATCC
EcoT14I 610 CCATGG C^C[AT][AT]GG
1874 CCAAGG
HaeII 1038 GGCGCT [GA]GCGC^[CT]
1766 AGCGCT
MflI 1553 AGATCC [GA]^GATC[CT]
1976 AGATCC
MluNI 1851 TGGCCA TGG^CCA
1905 TGGCCA
MscI 1851 TGGCCA TGG^CCA
1905 TGGCCA
PflMI 408 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1152 CCAGTACCTGG
PpuMI 1064 AGGACCT [GA]G^G[AT]CC[CT]
1943 GGGACCT
PvuII 1252 CAGCTG CAG^CTG
1384 CAGCTG
SbfI 1731 CCTGCAGG CCTGCA^GG
1863 CCTGCAGG
SgrAI 96 CGCCGGCG C[GA]^CCGG[CT]G
242 CGCCGGCG
Sse8387I 1731 CCTGCAGG CCTGCA^GG
1863 CCTGCAGG
StyI 610 CCATGG C^C[AT][AT]GG
1874 CCAAGG
TaqI 359 TCGA T^CGA
1754 TCGA
TfiI 1604 GAATC G^A[AT]TC
1992 GAATC
Tsp45I 736 GTCAC ^GT[GC]AC
883 GTGAC
TthHB8I 359 TCGA T^CGA
1754 TCGA
Van91I 408 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1152 CCAGTACCTGG
XhoII 1553 AGATCC [GA]^GATC[CT]
1976 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaII BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KspI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TseI TspRI