Restriction map for
pFN21AB7870
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AgeI AhdI AlwNI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbvCI BcgI BciVI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1371 GTCTAC GT^[AC][GT]AC
AflII 415 CTTAAG C^TTAAG
Alw44I 1637 GTGCAC G^TGCAC
AlwI 255 GATCC GGATC(4/5)
AocI 1042 CCTCAGG CC^T[ACGT]AGG
ApaLI 1637 GTGCAC G^TGCAC
AseI 946 ATTAAT AT^TAAT
AsnI 946 ATTAAT AT^TAAT
AvaII 1757 GGACC G^G[AT]CC
BalI 896 TGGCCA TGG^CCA
BanII 397 GAGCTC G[GA]GC[CT]^C
BbrPI 671 CACGTG CAC^GTG
BbsI 1449 GTCTTC GAAGAC(2/6)
BclI 507 TGATCA T^GATCA
BcnI 974 CCCGG CC^[GC]GG
BfrI 415 CTTAAG C^TTAAG
BglII 328 AGATCT A^GATCT
BpmI 1289 CTGGAG CTGGAG(16/14)
BpuAI 1449 GTCTTC GAAGAC(2/6)
BsaAI 671 CACGTG [CT]AC^GT[GA]
BsaI 1727 GAGACC GGTCTC(1/5)
BspMI 886 ACCTGC ACCTGC(4/8)
BstXI 798 CCATTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 328 AGATCT [GA]^GATC[CT]
Bsu36I 1042 CCTCAGG CC^T[ACGT]AGG
Cac8I 821 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 277 GCGC GCG^C
DraII 1718 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 896 TGGCCA [CT]^GGCC[GA]
Eco81I 1042 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1718 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1034 GATATC GAT^ATC
FauI 25 GCGGG CCCGC(4/6)
FbaI 507 TGATCA T^GATCA
HapII 975 CCGG C^CGG
HhaI 277 GCGC GCG^C
HinP1I 277 GCGC G^CGC
HincII 1258 GTCAAC GT[CT]^[GA]AC
HindII 1258 GTCAAC GT[CT]^[GA]AC
HindIII 531 AAGCTT A^AGCTT
HpaII 975 CCGG C^CGG
HphI 601 TCACC GGTGA(8/7)
MaeII 672 ACGT A^CGT
MflI 328 AGATCT [GA]^GATC[CT]
MluNI 896 TGGCCA TGG^CCA
MscI 896 TGGCCA TGG^CCA
MspI 975 CCGG C^CGG
MwoI 1681 GCCATGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 974 CCCGG CC^[GC]GG
PmaCI 671 CACGTG CAC^GTG
PmlI 671 CACGTG CAC^GTG
PshBI 946 ATTAAT AT^TAAT
SacI 397 GAGCTC GAGCT^C
SapI 739 GAAGAGC GCTCTTC(1/4)
XbaI 1339 TCTAGA T^CTAGA
XhoII 328 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 82 CGCG CG^CG
278 CGCG
AflIII 670 ACACGT A^C[GA][CT]GT
672 ACGTGT
Asp700I 161 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1072 GAATATTTTC
BbvI 19 GCTGC GCAGC(8/12)
1687 GCTGC
BsmAI 1727 GAGAC GTCTC(1/5)
487 GTCTC
BstUI 82 CGCG CG^CG
278 CGCG
Cfr13I 1719 GGGCC G^G[ACGT]CC
1757 GGACC
DraI 333 TTTAAA TTT^AAA
1194 TTTAAA
DsaI 272 CCATGG C^C[GA][CT]GG
1682 CCATGG
MvnI 82 CGCG CG^CG
278 CGCG
NcoI 272 CCATGG C^CATGG
1682 CCATGG
NlaIV 1718 GGGGCC GG[ACGT]^[ACGT]CC
1757 GGACCC
PleI 87 GAGTC GAGTC(4/5)
1473 GAGTC
PstI 422 CTGCAG CTGCA^G
1512 CTGCAG
Sau96I 1719 GGGCC G^G[ACGT]CC
1757 GGACC
ScaI 1383 AGTACT AGT^ACT
1594 AGTACT
SspI 832 AATATT AAT^ATT
1073 AATATT
TaqI 1471 TCGA T^CGA
1652 TCGA
TseI 19 GCTGC G^C[AT]GC
1687 GCTGC
Tsp45I 1664 GTGAC ^GT[GC]AC
1745 GTGAC
TthHB8I 1471 TCGA T^CGA
1652 TCGA
XmnI 161 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1072 GAATATTTTC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BfaI BmyI BsaJI BseRI
BsiHKAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII RsaI
Sau3AI ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I Tsp509I TspRI