Restriction map for
pFN21AE2388
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# NO CUT ENZYMES ( 73 enzymes) #
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AatII Acc65I AccIII AcyI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII BbeI BciVI BclI BfrI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsiWI BsmBI BspEI BspLU11I BssHII
BssSI BstBI BstXI CelII CpoI
DrdI Eco47III EcoT22I FbaI FspI
Hin1I HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NarI NheI NotI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
RsrII ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 3315 AACGTT AA^CGTT
AocI 2567 CCTCAGG CC^T[ACGT]AGG
AvrII 907 CCTAGG C^CTAGG
BalI 3788 TGGCCA TGG^CCA
BamHI 2179 GGATCC G^GATCC
BbrPI 2479 CACGTG CAC^GTG
BbvCI 4111 CCTCAGC CCTCAGC(-5/-2)
BglI 3302 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 845 AGATCT A^GATCT
BlnI 907 CCTAGG C^CTAGG
BsaAI 2479 CACGTG [CT]AC^GT[GA]
BsaWI 2880 ACCGGA [AT]^CCGG[AT]
BspDI 2339 ATCGAT AT^CGAT
BspHI 2498 TCATGA T^CATGA
Bst1107I 1557 GTATAC GTA^TAC
BstAPI 3850 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 765 GGTCACC G^GT[ACGT]ACC
BstPI 765 GGTCACC G^GT[ACGT]ACC
BstZ17I 1557 GTATAC GTA^TAC
Bsu36I 2567 CCTCAGG CC^T[ACGT]AGG
ClaI 2339 ATCGAT AT^CGAT
DraI 1932 TTTAAA TTT^AAA
Eco81I 2567 CCTCAGG CC^T[ACGT]AGG
EcoO65I 765 GGTCACC G^GT[ACGT]ACC
EcoRI 3441 GAATTC G^AATTC
EcoRV 214 GATATC GAT^ATC
FseI 4118 GGCCGGCC GGCCGG^CC
HincII 1149 GTCGAC GT[CT]^[GA]AC
HindII 1149 GTCGAC GT[CT]^[GA]AC
KspI 1852 CCGCGG CCGC^GG
MluNI 3788 TGGCCA TGG^CCA
MscI 3788 TGGCCA TGG^CCA
NaeI 4119 GCCGGC GCC^GGC
NdeI 2332 CATATG CA^TATG
NgoMIV 4119 GCCGGC G^CCGGC
NruI 780 TCGCGA TCG^CGA
PmaCI 2479 CACGTG CAC^GTG
PmlI 2479 CACGTG CAC^GTG
PshAI 695 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3315 AACGTT AA^CGTT
PvuI 2341 CGATCG CGAT^CG
PvuII 3446 CAGCTG CAG^CTG
RcaI 2498 TCATGA T^CATGA
SacII 1852 CCGCGG CCGC^GG
SalI 1149 GTCGAC G^TCGAC
SbfI 3474 CCTGCAGG CCTGCA^GG
SexAI 2952 ACCAGGT A^CC[AT]GGT
SmaI 2032 CCCGGG CCC^GGG
Sse8387I 3474 CCTGCAGG CCTGCA^GG
XbaI 1338 TCTAGA T^CTAGA
XmaCI 2032 CCCGGG C^CCGGG
XmaI 2032 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspI 382 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1682 GACGGAGTC
AvaI 2032 CCCGGG C^[CT]CG[GA]G
2492 CCCGAG
BsoBI 2032 CCCGGG C^[CT]CG[GA]G
2492 CCCGAG
Bsp1407I 491 TGTACA T^GTACA
4322 TGTACA
BsrDI 1600 GCAATG GCAATG(2/0)
3112 GCAATG
BsrFI 368 ACCGGC [GA]^CCGG[CT]
4119 GCCGGC
BsrGI 491 TGTACA T^GTACA
4322 TGTACA
Cfr10I 368 ACCGGC [GA]^CCGG[CT]
4119 GCCGGC
DraIII 2951 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4075 CACAGTGTG
EagI 23 CGGCCG C^GGCCG
4117 CGGCCG
EclXI 23 CGGCCG C^GGCCG
4117 CGGCCG
Eco52I 23 CGGCCG C^GGCCG
4117 CGGCCG
HaeII 1806 AGCGCC [GA]GCGC^[CT]
3208 GGCGCT
PflFI 382 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1682 GACGGAGTC
SmlI 570 CTCAAG C^T[CT][GA]AG
1217 CTCAAG
SspBI 491 TGTACA T^GTACA
4322 TGTACA
Tth111I 382 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1682 GACGGAGTC
XcmI 2849 CCAAGCCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3558 CCACCCACATTCTGG
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# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AhdI AluI AlwI AlwNI ApaI
ApoI AspEI AspHI AvaII BanI
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII