Restriction map for
pFN21AE3720
##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AccI AclI AgeI AscI Asp700I
AvrII BbeI BlnI BsiWI BspDI
BspHI BssHII Bst1107I BstZ17I ClaI
CpoI EcoNI FseI HpaI KasI
MfeI MluI MunI NarI NheI
NotI PacI PaeR7I PinAI PmeI
Psp1406I PvuI RcaI RsrII SalI
SbfI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3091 GGTACC G^GTACC
AccIII 606 TCCGGA T^CCGGA
AflII 4593 CTTAAG C^TTAAG
AhdI 4138 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1546 ATTAAT AT^TAAT
AsnI 1546 ATTAAT AT^TAAT
Asp718I 3091 GGTACC G^GTACC
AspEI 4138 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 764 TGCGCA TGC^GCA
BalI 735 TGGCCA TGG^CCA
BbrPI 2412 CACGTG CAC^GTG
BciVI 2784 GGATAC GTATCC(6/5)
BfaI 814 CTAG C^TAG
BfrI 4593 CTTAAG C^TTAAG
BglII 316 AGATCT A^GATCT
BseAI 606 TCCGGA T^CCGGA
BspEI 606 TCCGGA T^CCGGA
BspLU11I 2406 ACATGT A^CATGT
BstBI 5359 TTCGAA TT^CGAA
BstEII 4551 GGTCACC G^GT[ACGT]ACC
BstPI 4551 GGTCACC G^GT[ACGT]ACC
BstXI 7421 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 496 TTTAAA TTT^AAA
Eam1105I 4138 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4551 GGTCACC G^GT[ACGT]ACC
EcoRI 328 GAATTC G^AATTC
EcoT22I 2477 ATGCAT ATGCA^T
FspI 764 TGCGCA TGC^GCA
KpnI 3091 GGTACC GGTAC^C
MaeI 814 CTAG C^TAG
MluNI 735 TGGCCA TGG^CCA
MroI 606 TCCGGA T^CCGGA
MscI 735 TGGCCA TGG^CCA
NaeI 13 GCCGGC GCC^GGC
NcoI 2387 CCATGG C^CATGG
NdeI 6576 CATATG CA^TATG
NgoMIV 13 GCCGGC G^CCGGC
NruI 5484 TCGCGA TCG^CGA
NsiI 2477 ATGCAT ATGCA^T
NspV 5359 TTCGAA TT^CGAA
PmaCI 2412 CACGTG CAC^GTG
PmlI 2412 CACGTG CAC^GTG
PshBI 1546 ATTAAT AT^TAAT
SexAI 4754 ACCAGGT A^CC[AT]GGT
SfuI 5359 TTCGAA TT^CGAA
StuI 4843 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 3802 GACGTC GACGT^C
6157 GACGTC
AocI 4846 CCTGAGG CC^T[ACGT]AGG
6385 CCTCAGG
BbvCI 6821 CCTCAGC CCTCAGC(-5/-2)
7194 CCTCAGC
BclI 566 TGATCA T^GATCA
4826 TGATCA
BlpI 5249 GCTTAGC GC^T[ACGT]AGC
6927 GCTCAGC
Bpu1102I 5249 GCTTAGC GC^T[ACGT]AGC
6927 GCTCAGC
BsaBI 553 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2474 GATATGCATC
BsmBI 2871 CGTCTC CGTCTC(1/5)
2198 GAGACG
Bsp1407I 4772 TGTACA T^GTACA
6286 TGTACA
BsrFI 13 GCCGGC [GA]^CCGG[CT]
2563 ACCGGC
BsrGI 4772 TGTACA T^GTACA
6286 TGTACA
Bsu36I 4846 CCTGAGG CC^T[ACGT]AGG
6385 CCTCAGG
CelII 5249 GCTTAGC GC^T[ACGT]AGC
6927 GCTCAGC
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
2563 ACCGGC
EagI 15 CGGCCG C^GGCCG
5172 CGGCCG
EclXI 15 CGGCCG C^GGCCG
5172 CGGCCG
Eco52I 15 CGGCCG C^GGCCG
5172 CGGCCG
Eco81I 4846 CCTGAGG CC^T[ACGT]AGG
6385 CCTCAGG
FbaI 566 TGATCA T^GATCA
4826 TGATCA
KspI 3482 CCGCGG CCGC^GG
4002 CCGCGG
MamI 553 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2474 GATATGCATC
NspI 2406 ACATGT [GA]CATG^[CT]
4897 ACATGC
PflMI 3694 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6162 CCAGGTCCTGG
PshAI 4375 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5370 GACTCCTGTC
SacII 3482 CCGCGG CCGC^GG
4002 CCGCGG
ScaI 5239 AGTACT AGT^ACT
6641 AGTACT
SspBI 4772 TGTACA T^GTACA
6286 TGTACA
Van91I 3694 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6162 CCAGGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI ApoI AspHI
AspI AvaI AvaII BamHI BanI
BanII BbsI BbvI BcgI BcnI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI Eco47III EcoO109I EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII
XmaCI XmaI