Restriction map for
pFN21AE5834
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MaeII
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TspRI Tth111I Van91I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 962 GTCGAC GT^[AC][GT]AC
Alw44I 1094 GTGCAC G^TGCAC
ApaLI 1094 GTGCAC G^TGCAC
Asp700I 308 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 383 GGCACC G^G[CT][GA]CC
BbvI 190 GCTGC GCAGC(8/12)
BglII 328 AGATCT A^GATCT
BpmI 606 CTGGAG CTGGAG(16/14)
BsaBI 655 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 282 GAGACC GGTCTC(1/5)
BseRI 482 CTCCTC GAGGAG(10/8)
BsmI 186 GAATGC GAATGC(1/-1)
BspHI 996 TCATGA T^CATGA
BsrI 445 CCAGT ACTGG(1/-1)
BstAPI 1096 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 328 AGATCT [GA]^GATC[CT]
Cac8I 23 GCAAGC GC[ACGT]^[ACGT]GC
EarI 560 GAAGAG CTCTTC(1/4)
EcoNI 1102 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 190 GCTGC GC^[ACGT]GC
HindIII 25 AAGCTT A^AGCTT
ItaI 190 GCTGC GC^[ACGT]GC
Ksp632I 560 GAAGAG CTCTTC(1/4)
MamI 655 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 328 AGATCT [GA]^GATC[CT]
NcoI 933 CCATGG C^CATGG
PstI 173 CTGCAG CTGCA^G
RcaI 996 TCATGA T^CATGA
SalI 962 GTCGAC G^TCGAC
TfiI 1254 GAATC G^A[AT]TC
TseI 190 GCTGC G^C[AT]GC
Tsp45I 552 GTCAC ^GT[GC]AC
Tsp509I 568 AATT ^AATT
XhoII 328 AGATCT [GA]^GATC[CT]
XmnI 308 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 1258 CCGC CCGC(-3/-1)
279 GCGG
AlwI 1285 GATCC GGATC(4/5)
123 GGATC
AspHI 1094 GTGCAC G[AT]GC[AT]^C
1276 GTGCTC
BsiHKAI 1094 GTGCAC G[AT]GC[AT]^C
1276 GTGCTC
BsmAI 282 GAGAC GTCTC(1/5)
1361 GAGAC
BspMI 1101 ACCTGC ACCTGC(4/8)
1138 ACCTGC
BssSI 1160 CACGAG CACGAG(-5/-1)
1289 CTCGTG
DsaI 345 CCGTGG C^C[GA][CT]GG
933 CCATGG
EcoT22I 713 ATGCAT ATGCA^T
824 ATGCAT
HincII 624 GTCAAC GT[CT]^[GA]AC
962 GTCGAC
HindII 624 GTCAAC GT[CT]^[GA]AC
962 GTCGAC
HphI 1009 GGTGA GGTGA(8/7)
1204 GGTGA
MaeIII 552 GTCAC ^GT[ACGT]AC
1190 GTTAC
MseI 71 TTAA T^TAA
449 TTAA
NsiI 713 ATGCAT ATGCA^T
824 ATGCAT
PaeR7I 1194 CTCGAG C^TCGAG
1365 CTCGAG
SfcI 173 CTGCAG C^T[GA][CT]AG
1325 CTATAG
Tru9I 71 TTAA T^TAA
449 TTAA
XhoI 1194 CTCGAG C^TCGAG
1365 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI AvaI AvaII BciVI BfaI
BmyI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FokI HaeIII HinfI
MaeI MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TthHB8I XbaI