Restriction map for
pFN21AE6512
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MroI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tsp509I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 81 ACGCGT A^C[GA][CT]GT
AlwI 402 GATCC GGATC(4/5)
Aor51HI 197 AGCGCT AGC^GCT
BalI 395 TGGCCA TGG^CCA
BanI 244 GGCACC G^G[CT][GA]CC
BanII 448 GAGCCC G[GA]GC[CT]^C
BcnI 223 CCGGG CC^[GC]GG
BmyI 448 GAGCCC G[AGT]GC[ACT]^C
BpmI 367 CTGGAG CTGGAG(16/14)
Bsp1286I 448 GAGCCC G[AGT]GC[ACT]^C
BspHI 281 TCATGA T^CATGA
BsrI 257 ACTGG ACTGG(1/-1)
BstEII 231 GGTCACC G^GT[ACGT]ACC
BstPI 231 GGTCACC G^GT[ACGT]ACC
BstYI 401 AGATCC [GA]^GATC[CT]
DpnII 402 GATC ^GATC
DraII 56 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 470 CCGTGG C^C[GA][CT]GG
EaeI 395 TGGCCA [CT]^GGCC[GA]
Eco47III 197 AGCGCT AGC^GCT
EcoNI 48 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 56 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 231 GGTCACC G^GT[ACGT]ACC
HapII 223 CCGG C^CGG
HpaII 223 CCGG C^CGG
MboI 402 GATC ^GATC
MboII 314 TCTTC GAAGA(8/7)
MflI 401 AGATCC [GA]^GATC[CT]
MluI 81 ACGCGT A^CGCGT
MluNI 395 TGGCCA TGG^CCA
MscI 395 TGGCCA TGG^CCA
MseI 479 TTAA T^TAA
MspI 223 CCGG C^CGG
NciI 223 CCGGG CC^[GC]GG
NdeII 402 GATC ^GATC
NspI 35 ACATGC [GA]CATG^[CT]
PstI 136 CTGCAG CTGCA^G
RcaI 281 TCATGA T^CATGA
Sau3AI 402 GATC ^GATC
SmlI 460 CTCAAG C^T[CT][GA]AG
StuI 464 AGGCCT AGG^CCT
TaqI 194 TCGA T^CGA
Tru9I 479 TTAA T^TAA
TspRI 306 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 194 TCGA T^CGA
XhoII 401 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 82 CGCG CG^CG
103 CGCG
AcyI 261 GACGCC G[GA]^CG[CT]C
361 GACGCC
AfaI 156 GTAC GT^AC
417 GTAC
AlwNI 55 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
292 CAGCTGCTG
BsaHI 261 GACGCC G[GA]^CG[CT]C
361 GACGCC
BstF5I 120 GGATG GGATG(2/0)
336 GGATG
BstUI 82 CGCG CG^CG
103 CGCG
FauI 451 CCCGC CCCGC(4/6)
104 GCGGG
FokI 120 GGATG GGATG(9/13)
336 GGATG
HgaI 261 GACGC GACGC(5/10)
361 GACGC
Hin1I 261 GACGCC G[GA]^CG[CT]C
361 GACGCC
HinfI 148 GAGTC G^A[ACGT]TC
331 GACTC
MaeIII 232 GTCAC ^GT[ACGT]AC
427 GTCAC
MvnI 82 CGCG CG^CG
103 CGCG
PleI 331 GACTC GAGTC(4/5)
148 GAGTC
PvuII 28 CAGCTG CAG^CTG
292 CAGCTG
RsaI 156 GTAC GT^AC
417 GTAC
SfaNI 72 GATGC GCATC(5/9)
337 GATGC
SfcI 136 CTGCAG C^T[GA][CT]AG
187 CTACAG
Tsp45I 232 GTCAC ^GT[GC]AC
427 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsiYI BslI BssKI BstNI Cac8I
CfoI Cfr13I EcoRII Fnu4HI HaeII
HaeIII HhaI HinP1I HphI ItaI
MnlI MspA1I MvaI MwoI NlaIII
NlaIV PflMI Sau96I ScrFI TseI
Van91I