Restriction map for
pFN21AE4357
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FauI FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspI StuI
SwaI TaqI Tsp509I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 901 GCGG CCGC(-3/-1)
AflIII 467 ACATGT A^C[GA][CT]GT
AhdI 910 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 967 CCTCAGG CC^T[ACGT]AGG
AspEI 910 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 362 TGATCA T^GATCA
BcnI 452 CCCGG CC^[GC]GG
BsaI 237 GGTCTC GGTCTC(1/5)
BseRI 853 CTCCTC GAGGAG(10/8)
BsiEI 773 CGACCG CG[GA][CT]^CG
BsmBI 777 CGTCTC CGTCTC(1/5)
Bsp1407I 1070 TGTACA T^GTACA
BspLU11I 467 ACATGT A^CATGT
BspMI 806 ACCTGC ACCTGC(4/8)
BsrGI 1070 TGTACA T^GTACA
BsrI 341 CCAGT ACTGG(1/-1)
BstYI 1099 AGATCC [GA]^GATC[CT]
Bsu36I 967 CCTCAGG CC^T[ACGT]AGG
Cac8I 784 GCATGC GC[ACGT]^[ACGT]GC
CfoI 210 GCGC GCG^C
Eam1105I 910 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 967 CCTCAGG CC^T[ACGT]AGG
EcoRV 1060 GATATC GAT^ATC
FbaI 362 TGATCA T^GATCA
HapII 453 CCGG C^CGG
HgaI 270 GACGC GACGC(5/10)
HhaI 210 GCGC GCG^C
HinP1I 210 GCGC G^CGC
HincII 529 GTCAAC GT[CT]^[GA]AC
HindII 529 GTCAAC GT[CT]^[GA]AC
HindIII 740 AAGCTT A^AGCTT
HpaII 453 CCGG C^CGG
MflI 1099 AGATCC [GA]^GATC[CT]
MseI 197 TTAA T^TAA
MspA1I 400 CAGCTG C[AC]G^C[GT]G
MspI 453 CCGG C^CGG
MwoI 449 GCTCCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 452 CCCGG CC^[GC]GG
PflFI 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 400 CAGCTG CAG^CTG
SacI 456 GAGCTC GAGCT^C
SbfI 514 CCTGCAGG CCTGCA^GG
SmlI 762 CTCAAG C^T[CT][GA]AG
SphI 784 GCATGC GCATG^C
Sse8387I 514 CCTGCAGG CCTGCA^GG
SspBI 1070 TGTACA T^GTACA
TfiI 993 GAATC G^A[AT]TC
Tru9I 197 TTAA T^TAA
Tth111I 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 341 CCAGTGACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1099 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 471 GTAC GT^AC
1071 GTAC
AlwI 769 GATCC GGATC(4/5)
1100 GATCC
Asp700I 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
665 GAAACCCTTC
AspHI 456 GAGCTC G[AT]GC[AT]^C
879 GTGCTC
BalI 58 TGGCCA TGG^CCA
735 TGGCCA
BsgI 178 CTGCAC GTGCAG(16/14)
808 CTGCAC
BsiHKAI 456 GAGCTC G[AT]GC[AT]^C
879 GTGCTC
BstXI 410 CCACGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
616 CCATACGAGTGG
DsaI 410 CCACGG C^C[GA][CT]GG
573 CCACGG
EaeI 58 TGGCCA [CT]^GGCC[GA]
735 TGGCCA
EcoT14I 242 CCAAGG C^C[AT][AT]GG
524 CCAAGG
MaeII 504 ACGT A^CGT
731 ACGT
MluNI 58 TGGCCA TGG^CCA
735 TGGCCA
MscI 58 TGGCCA TGG^CCA
735 TGGCCA
MslI 226 CACCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
617 CATACGAGTG
NspI 467 ACATGT [GA]CATG^[CT]
784 GCATGC
RsaI 471 GTAC GT^AC
1071 GTAC
SfaNI 296 GATGC GCATC(5/9)
590 GATGC
StyI 242 CCAAGG C^C[AT][AT]GG
524 CCAAGG
XmnI 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
665 GAAACCCTTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI ApaI AvaII BanII BbvI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MvaI NdeII
NlaIII NlaIV PleI PpuMI PstI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp45I TspRI