Restriction map for
pFN21AE9641
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# NO CUT ENZYMES ( 165 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HapII HgaI
HhaI HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspI SwaI Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 858 GACGTC GACGT^C
AcyI 858 GACGTC G[GA]^CG[CT]C
AvaII 826 GGACC G^G[AT]CC
BglII 135 AGATCT A^GATCT
BsaAI 982 TACGTA [CT]AC^GT[GA]
BsaHI 858 GACGTC G[GA]^CG[CT]C
BsgI 206 CTGCAC GTGCAG(16/14)
BsmI 485 GAATGC GAATGC(1/-1)
BspHI 1077 TCATGA T^CATGA
BstYI 135 AGATCT [GA]^GATC[CT]
Cfr13I 826 GGACC G^G[ACGT]CC
DrdI 858 GACGTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1025 CCACGG C^C[GA][CT]GG
EarI 17 CTCTTC CTCTTC(1/4)
EcoT14I 245 CCAAGG C^C[AT][AT]GG
Hin1I 858 GACGTC G[GA]^CG[CT]C
HindIII 113 AAGCTT A^AGCTT
HphI 376 GGTGA GGTGA(8/7)
Ksp632I 17 CTCTTC CTCTTC(1/4)
MflI 135 AGATCT [GA]^GATC[CT]
NdeI 104 CATATG CA^TATG
NlaIII 1078 CATG CATG^
NlaIV 1129 GGTTCC GG[ACGT]^[ACGT]CC
RcaI 1077 TCATGA T^CATGA
Sau96I 826 GGACC G^G[ACGT]CC
SfaNI 258 GATGC GCATC(5/9)
SnaBI 982 TACGTA TAC^GTA
StuI 734 AGGCCT AGG^CCT
StyI 245 CCAAGG C^C[AT][AT]GG
TfiI 501 GAATC G^A[AT]TC
Tsp509I 78 AATT ^AATT
XhoII 135 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 337 GTAGAC GT^[AC][GT]AC
1015 GTAGAC
AlwNI 679 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
764 CAGCTTCTG
Asp700I 13 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
612 GAATGACTTC
BfaI 238 CTAG C^TAG
667 CTAG
BpmI 917 CTCCAG CTGGAG(16/14)
1071 CTCCAG
BsmAI 691 GAGAC GTCTC(1/5)
729 GTCTC
BsmFI 531 GGGAC GGGAC(10/14)
525 GTCCC
Bsp1407I 108 TGTACA T^GTACA
1170 TGTACA
BsrGI 108 TGTACA T^GTACA
1170 TGTACA
Cac8I 208 GCACGC GC[ACGT]^[ACGT]GC
698 GCCAGC
MaeI 238 CTAG C^TAG
667 CTAG
MaeIII 154 GTAAC ^GT[ACGT]AC
980 GTTAC
MspA1I 520 CAGCTG C[AC]G^C[GT]G
682 CAGCTG
PshAI 805 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1069 GACTCCAGTC
PstI 673 CTGCAG CTGCA^G
807 CTGCAG
PvuII 520 CAGCTG CAG^CTG
682 CAGCTG
SspBI 108 TGTACA T^GTACA
1170 TGTACA
XcmI 852 CCAGAGGACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1137 CCACGTTCAGAATGG
XmnI 13 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
612 GAATGACTTC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BseRI BsiYI BslI BsrI BssKI
BstF5I BstNI DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HinfI
ItaI MaeII MboI MboII MnlI
MseI MvaI MwoI NdeII PflMI
PleI RsaI Sau3AI ScrFI SfcI
SmlI TaqI Tru9I TseI TspRI
TthHB8I Van91I