Restriction map for
pFN21AE4312
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
TfiI Tsp45I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 592 GTAC GT^AC
AlwI 153 GATCC GGATC(4/5)
AspI 827 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 171 GGACC G^G[AT]CC
BalI 575 TGGCCA TGG^CCA
BclI 308 TGATCA T^GATCA
BcnI 36 CCGGG CC^[GC]GG
BpmI 80 CTGGAG CTGGAG(16/14)
BsmAI 98 GAGAC GTCTC(1/5)
Bsp1407I 591 TGTACA T^GTACA
BsrGI 591 TGTACA T^GTACA
BstF5I 421 GGATG GGATG(2/0)
BstXI 614 CCAAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 152 AGATCC [GA]^GATC[CT]
Cfr13I 171 GGACC G^G[ACGT]CC
DsaI 13 CCGTGG C^C[GA][CT]GG
EaeI 575 TGGCCA [CT]^GGCC[GA]
EarI 6 CTCTTC CTCTTC(1/4)
EcoRI 90 GAATTC G^AATTC
EcoT14I 252 CCTTGG C^C[AT][AT]GG
FbaI 308 TGATCA T^GATCA
FokI 421 GGATG GGATG(9/13)
HaeII 940 GGCGCT [GA]GCGC^[CT]
HapII 36 CCGG C^CGG
HincII 298 GTTGAC GT[CT]^[GA]AC
HindII 298 GTTGAC GT[CT]^[GA]AC
HinfI 827 GACTC G^A[ACGT]TC
HpaII 36 CCGG C^CGG
HphI 333 TCACC GGTGA(8/7)
Ksp632I 6 CTCTTC CTCTTC(1/4)
MaeII 767 ACGT A^CGT
MflI 152 AGATCC [GA]^GATC[CT]
MluNI 575 TGGCCA TGG^CCA
MscI 575 TGGCCA TGG^CCA
MspI 36 CCGG C^CGG
NciI 36 CCGGG CC^[GC]GG
NlaIV 18 GGAGCC GG[ACGT]^[ACGT]CC
PflFI 827 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 827 GACTC GAGTC(4/5)
PstI 560 CTGCAG CTGCA^G
RsaI 592 GTAC GT^AC
Sau96I 171 GGACC G^G[ACGT]CC
SspBI 591 TGTACA T^GTACA
StyI 252 CCTTGG C^C[AT][AT]GG
Tth111I 827 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 174 CCAGGCATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 152 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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BfaI 905 CTAG C^TAG
937 CTAG
BsaJI 13 CCGTGG C^C[ACGT][ACGT]GG
252 CCTTGG
BseRI 344 CTCCTC GAGGAG(10/8)
866 GAGGAG
BstNI 79 CCTGG CC^[AT]GG
174 CCAGG
CfoI 33 GCGC GCG^C
941 GCGC
DdeI 86 CTGAG C^T[ACGT]AG
347 CTCAG
DpnII 153 GATC ^GATC
309 GATC
EcoRII 79 CCTGG ^CC[AT]GG
174 CCAGG
HhaI 33 GCGC GCG^C
941 GCGC
HinP1I 33 GCGC G^CGC
941 GCGC
MaeI 905 CTAG C^TAG
937 CTAG
MboI 153 GATC ^GATC
309 GATC
MseI 446 TTAA T^TAA
686 TTAA
MvaI 79 CCTGG CC^[AT]GG
174 CCAGG
NdeI 376 CATATG CA^TATG
878 CATATG
NdeII 153 GATC ^GATC
309 GATC
NspI 178 GCATGT [GA]CATG^[CT]
294 GCATGT
Sau3AI 153 GATC ^GATC
309 GATC
SfaNI 145 GATGC GCATC(5/9)
499 GCATC
SfcI 560 CTGCAG C^T[GA][CT]AG
944 CTACAG
Tru9I 446 TTAA T^TAA
686 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsiYI
BslI BsmFI BsrI BssKI Cac8I
Fnu4HI HaeIII ItaI MboII MnlI
MwoI NlaIII ScrFI TseI Tsp509I
TspRI