Restriction map for
pFN21AE3081
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TaqI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 433 CGCG CG^CG
AcyI 290 GGCGCC G[GA]^CG[CT]C
AfaI 234 GTAC GT^AC
ApaI 570 GGGCCC GGGCC^C
AspHI 631 GAGCTC G[AT]GC[AT]^C
AvaI 644 CTCGGG C^[CT]CG[GA]G
BbeI 290 GGCGCC GGCGC^C
BsaHI 290 GGCGCC G[GA]^CG[CT]C
BsgI 619 CTGCAC GTGCAG(16/14)
BsiHKAI 631 GAGCTC G[AT]GC[AT]^C
BsmAI 314 GTCTC GTCTC(1/5)
BsmBI 313 CGTCTC CGTCTC(1/5)
BsmFI 370 GGGAC GGGAC(10/14)
BsoBI 644 CTCGGG C^[CT]CG[GA]G
BspMI 215 ACCTGC ACCTGC(4/8)
BsrI 552 ACTGG ACTGG(1/-1)
BstAPI 120 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 401 GGATG GGATG(2/0)
BstUI 433 CGCG CG^CG
BstXI 416 CCATGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 436 GATC ^GATC
EarI 505 GAAGAG CTCTTC(1/4)
EcoNI 483 CCTCCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 654 GCGGG CCCGC(4/6)
FokI 401 GGATG GGATG(9/13)
HgaI 312 GCGTC GACGC(5/10)
Hin1I 290 GGCGCC G[GA]^CG[CT]C
HphI 213 TCACC GGTGA(8/7)
KasI 290 GGCGCC G^GCGCC
Ksp632I 505 GAAGAG CTCTTC(1/4)
MboI 436 GATC ^GATC
MboII 505 GAAGA GAAGA(8/7)
MvnI 433 CGCG CG^CG
NarI 290 GGCGCC GG^CGCC
NdeII 436 GATC ^GATC
PflMI 459 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 370 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 234 GTAC GT^AC
SacI 631 GAGCTC GAGCT^C
Sau3AI 436 GATC ^GATC
SbfI 117 CCTGCAGG CCTGCA^GG
SfoI 290 GGCGCC GGC^GCC
Sse8387I 117 CCTGCAGG CCTGCA^GG
StuI 284 AGGCCT AGG^CCT
TspRI 551 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 459 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AlwNI 121 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
460 CAGGTGCTG
BanI 290 GGCGCC G^G[CT][GA]CC
446 GGCACC
BlpI 33 GCTCAGC GC^T[ACGT]AGC
111 GCTGAGC
Bpu1102I 33 GCTCAGC GC^T[ACGT]AGC
111 GCTGAGC
CelII 33 GCTCAGC GC^T[ACGT]AGC
111 GCTGAGC
CfoI 291 GCGC GCG^C
560 GCGC
HaeII 290 GGCGCC [GA]GCGC^[CT]
559 AGCGCC
HhaI 291 GCGC GCG^C
560 GCGC
HinP1I 291 GCGC G^CGC
560 GCGC
PvuII 109 CAGCTG CAG^CTG
406 CAGCTG
SfaNI 402 GATGC GCATC(5/9)
153 GCATC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HapII HinfI
HpaII ItaI MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PstI Sau96I ScrFI SfcI StyI
TfiI TseI