Restriction map for
pFN21AE5538
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AviII BalI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
Fnu4HI FseI FspI HaeII HgaI
Hin1I HpaI ItaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I TseI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 995 CGCG CG^CG
Asp700I 720 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1078 GTGCTC G[AT]GC[AT]^C
AspI 906 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 27 CCTAGG C^CTAGG
BamHI 452 GGATCC G^GATCC
BanI 970 GGCACC G^G[CT][GA]CC
BlnI 27 CCTAGG C^CTAGG
BseRI 145 CTCCTC GAGGAG(10/8)
BsiHKAI 1078 GTGCTC G[AT]GC[AT]^C
Bsp1407I 551 TGTACA T^GTACA
BspHI 797 TCATGA T^CATGA
BsrDI 431 GCAATG GCAATG(2/0)
BsrGI 551 TGTACA T^GTACA
BssSI 490 CACGAG CACGAG(-5/-1)
BstUI 995 CGCG CG^CG
BstXI 512 CCAACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 452 GGATCC [GA]^GATC[CT]
DrdI 787 GACTTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 474 GAATTC G^AATTC
FauI 993 CCCGC CCCGC(4/6)
HaeIII 115 GGCC GG^CC
HindIII 691 AAGCTT A^AGCTT
MaeII 964 ACGT A^CGT
MflI 452 GGATCC [GA]^GATC[CT]
MvnI 995 CGCG CG^CG
MwoI 188 GCAGAGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 593 ACATGC [GA]CATG^[CT]
PflFI 906 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 126 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 797 TCATGA T^CATGA
SapI 723 GCTCTTC GCTCTTC(1/4)
SexAI 373 ACCTGGT A^CC[AT]GGT
SspBI 551 TGTACA T^GTACA
Tth111I 906 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 126 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 956 CCATGACCACGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 452 GGATCC [GA]^GATC[CT]
XmnI 720 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 378 GTAC GT^AC
552 GTAC
AlwNI 127 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
908 CAGAGTCTG
BbsI 22 GTCTTC GAAGAC(2/6)
1045 GTCTTC
BcnI 456 CCCGG CC^[GC]GG
923 CCCGG
BmyI 969 GGGCAC G[AGT]GC[ACT]^C
1078 GTGCTC
BpmI 46 CTCCAG CTGGAG(16/14)
917 CTCCAG
BpuAI 22 GTCTTC GAAGAC(2/6)
1045 GTCTTC
BsmFI 63 GGGAC GGGAC(10/14)
856 GTCCC
Bsp1286I 969 GGGCAC G[AGT]GC[ACT]^C
1078 GTGCTC
Cac8I 443 GCCAGC GC[ACGT]^[ACGT]GC
821 GCAGGC
CfoI 258 GCGC GCG^C
1058 GCGC
DraII 63 GGGACCT [GA]G^G[ACGT]CC[CT]
484 AGGACCC
EarI 93 CTCTTC CTCTTC(1/4)
724 CTCTTC
EcoNI 147 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
464 CCTTTTTTAGG
EcoO109I 63 GGGACCT [GA]G^G[ACGT]CC[CT]
484 AGGACCC
HhaI 258 GCGC GCG^C
1058 GCGC
HinP1I 258 GCGC G^CGC
1058 GCGC
HincII 165 GTCAAC GT[CT]^[GA]AC
307 GTCAAC
HindII 165 GTCAAC GT[CT]^[GA]AC
307 GTCAAC
Ksp632I 93 CTCTTC CTCTTC(1/4)
724 CTCTTC
MslI 490 CACGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
792 CATGGTCATG
NciI 456 CCCGG CC^[GC]GG
923 CCCGG
PleI 412 GAGTC GAGTC(4/5)
910 GAGTC
PpuMI 63 GGGACCT [GA]G^G[AT]CC[CT]
484 AGGACCC
RsaI 378 GTAC GT^AC
552 GTAC
TfiI 76 GAATC G^A[AT]TC
613 GATTC
TspRI 282 CACTG CA[GC]TG[ACGT][ACGT]^
291 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BfaI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII EcoT14I
FokI HapII HinfI HpaII HphI
MaeI MaeIII MboI MboII MnlI
MspI MvaI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI Tsp45I Tsp509I TthHB8I