Restriction map for
pFN21AE5251
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglI BglII BlnI BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstNI BstPI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HaeIII Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvaI NaeI NarI
NcoI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TaqI TfiI TthHB8I
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 464 GAATTC [GA]^AATT[CT]
AfaI 451 GTAC GT^AC
AflIII 492 ACACGT A^C[GA][CT]GT
AluI 350 AGCT AG^CT
ApoI 464 GAATTC [GA]^AATT[CT]
AspI 432 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 166 GGACC G^G[AT]CC
BcgI 347 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 111 CCCGG CC^[GC]GG
BlpI 45 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC
BsaAI 493 CACGTA [CT]AC^GT[GA]
BsaI 403 GGTCTC GGTCTC(1/5)
BsgI 121 CTGCAC GTGCAG(16/14)
BsmBI 126 CGTCTC CGTCTC(1/5)
BsrI 163 ACTGG ACTGG(1/-1)
BssKI 111 CCCGG ^CC[ACGT]GG
Cac8I 18 GCGGGC GC[ACGT]^[ACGT]GC
CelII 45 GCTGAGC GC^T[ACGT]AGC
CfoI 61 GCGC GCG^C
Cfr13I 166 GGACC G^G[ACGT]CC
DdeI 46 CTGAG C^T[ACGT]AG
DrdI 100 GACAGGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 531 GAAGAG CTCTTC(1/4)
EcoRI 464 GAATTC G^AATTC
FauI 18 GCGGG CCCGC(4/6)
HaeII 60 GGCGCT [GA]GCGC^[CT]
HapII 112 CCGG C^CGG
HgaI 107 GCGTC GACGC(5/10)
HhaI 61 GCGC GCG^C
HinP1I 61 GCGC G^CGC
HpaII 112 CCGG C^CGG
Ksp632I 531 GAAGAG CTCTTC(1/4)
MseI 245 TTAA T^TAA
MspI 112 CCGG C^CGG
NciI 111 CCCGG CC^[GC]GG
NdeI 518 CATATG CA^TATG
NlaIII 138 CATG CATG^
PflFI 432 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 454 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 451 GTAC GT^AC
Sau96I 166 GGACC G^G[ACGT]CC
ScrFI 111 CCCGG CC^[ACGT]GG
SmlI 406 CTCAAG C^T[CT][GA]AG
Tru9I 245 TTAA T^TAA
Tsp509I 465 AATT ^AATT
Tth111I 432 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 454 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 219 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 17 CGCG CG^CG
131 CGCG
AlwI 134 GGATC GGATC(4/5)
484 GGATC
BfaI 170 CTAG C^TAG
220 CTAG
BsiYI 434 CCCTGTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
454 CCAAATGCTGG
BslI 434 CCCTGTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
454 CCAAATGCTGG
BsmAI 127 GTCTC GTCTC(1/5)
404 GTCTC
BstUI 17 CGCG CG^CG
131 CGCG
MaeI 170 CTAG C^TAG
220 CTAG
MaeIII 424 GTCAC ^GT[ACGT]AC
478 GTGAC
MboII 154 GAAGA GAAGA(8/7)
531 GAAGA
MnlI 472 CCTC CCTC(7/6)
335 GAGG
MvnI 17 CGCG CG^CG
131 CGCG
MwoI 123 GCACGTCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
132 GCGGATCATGC
SspI 275 AATATT AAT^ATT
377 AATATT
Tsp45I 424 GTCAC ^GT[GC]AC
478 GTGAC
TspRI 29 CACTG CA[GC]TG[ACGT][ACGT]^
162 CACTG
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AciI BbvI BsmFI DpnII Fnu4HI
HphI ItaI MaeII MboI NdeII
Sau3AI TseI