Restriction map for
pFN21AE5313
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DpnII
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRV EcoT22I
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI ItaI KasI KpnI Ksp632I
KspI MamI MboI MboII MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NciI NcoI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SapI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TseI Tsp45I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 130 GTCGAC GT^[AC][GT]AC
AccII 173 CGCG CG^CG
AcsI 373 GAATTC [GA]^AATT[CT]
Alw44I 527 GTGCAC G^TGCAC
AlwNI 580 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 610 CCTGAGG CC^T[ACGT]AGG
ApaLI 527 GTGCAC G^TGCAC
ApoI 373 GAATTC [GA]^AATT[CT]
Asp700I 507 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 527 GTGCAC G[AT]GC[AT]^C
BciVI 215 GTATCC GTATCC(6/5)
BfaI 649 CTAG C^TAG
BmyI 527 GTGCAC G[AGT]GC[ACT]^C
BpmI 201 CTCCAG CTGGAG(16/14)
BseRI 644 CTCCTC GAGGAG(10/8)
BsiHKAI 527 GTGCAC G[AT]GC[AT]^C
BsmI 73 GCATTC GAATGC(1/-1)
Bsp1286I 527 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 117 ACATGT A^CATGT
BspMI 314 ACCTGC ACCTGC(4/8)
BssKI 69 CCAGG ^CC[ACGT]GG
BstNI 69 CCAGG CC^[AT]GG
BstUI 173 CGCG CG^CG
Bsu36I 610 CCTGAGG CC^T[ACGT]AGG
Cac8I 163 GCGGGC GC[ACGT]^[ACGT]GC
DsaI 541 CCGTGG C^C[GA][CT]GG
Eco81I 610 CCTGAGG CC^T[ACGT]AGG
EcoRI 373 GAATTC G^AATTC
EcoRII 69 CCAGG ^CC[AT]GG
EcoT14I 532 CCTTGG C^C[AT][AT]GG
FauI 163 GCGGG CCCGC(4/6)
HapII 729 CCGG C^CGG
HincII 130 GTCGAC GT[CT]^[GA]AC
HindII 130 GTCGAC GT[CT]^[GA]AC
HpaII 729 CCGG C^CGG
HphI 260 TCACC GGTGA(8/7)
MaeI 649 CTAG C^TAG
MspA1I 161 CAGCGG C[AC]G^C[GT]G
MspI 729 CCGG C^CGG
MvaI 69 CCAGG CC^[AT]GG
MvnI 173 CGCG CG^CG
NspI 117 ACATGT [GA]CATG^[CT]
PleI 725 GAGTC GAGTC(4/5)
SalI 130 GTCGAC G^TCGAC
ScrFI 69 CCAGG CC^[ACGT]GG
SmlI 20 CTCAAG C^T[CT][GA]AG
StyI 532 CCTTGG C^C[AT][AT]GG
TspRI 503 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 507 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AciI 163 GCGG CCGC(-3/-1)
174 GCGG
AflIII 117 ACATGT A^C[GA][CT]GT
814 ACGTGT
AvaII 536 GGACC G^G[AT]CC
640 GGACC
BsmAI 659 GAGAC GTCTC(1/5)
582 GTCTC
Bsp1407I 120 TGTACA T^GTACA
817 TGTACA
BsrGI 120 TGTACA T^GTACA
817 TGTACA
BsrI 302 ACTGG ACTGG(1/-1)
662 ACTGG
BstXI 35 CCATAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
741 CCATGTGTTTGG
EcoNI 16 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
643 CCTCCTCTAGG
HaeIII 166 GGCC GG^CC
731 GGCC
MaeII 796 ACGT A^CGT
814 ACGT
MslI 36 CATAACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
624 CATACTCATG
MwoI 267 GCTGGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
723 GCGAGTCCGGC
SspBI 120 TGTACA T^GTACA
817 TGTACA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BsmFI BspHI BstF5I Cfr13I DdeI
FokI HinfI MaeIII MnlI MseI
NdeI NlaIII NlaIV RcaI RsaI
Sau96I SfaNI TaqI TfiI Tru9I
Tsp509I TthHB8I XcmI