Restriction map for
pFN21AE2541
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI Ksp632I KspI MaeI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 202 GTCGAC GT^[AC][GT]AC
AfaI 50 GTAC GT^AC
AgeI 198 ACCGGT A^CCGGT
Alw44I 315 GTGCAC G^TGCAC
ApaI 706 GGGCCC GGGCC^C
ApaLI 315 GTGCAC G^TGCAC
AspHI 315 GTGCAC G[AT]GC[AT]^C
BamHI 160 GGATCC G^GATCC
BanI 625 GGTGCC G^G[CT][GA]CC
BanII 706 GGGCCC G[GA]GC[CT]^C
BbvCI 150 CCTCAGC CCTCAGC(-5/-2)
BcnI 915 CCGGG CC^[GC]GG
BsaBI 327 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 475 GGTCTC GGTCTC(1/5)
BsaWI 198 ACCGGT [AT]^CCGG[AT]
BsgI 253 GTGCAG GTGCAG(16/14)
BsiEI 200 CGGTCG CG[GA][CT]^CG
BsiHKAI 315 GTGCAC G[AT]GC[AT]^C
BsmBI 862 GAGACG CGTCTC(1/5)
BspMI 657 ACCTGC ACCTGC(4/8)
BsrDI 240 CATTGC GCAATG(2/0)
BsrFI 198 ACCGGT [GA]^CCGG[CT]
BstF5I 867 GGATG GGATG(2/0)
BstXI 416 CCAGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 160 GGATCC [GA]^GATC[CT]
Cfr10I 198 ACCGGT [GA]^CCGG[CT]
DraI 784 TTTAAA TTT^AAA
EcoRI 769 GAATTC G^AATTC
FauI 640 CCCGC CCCGC(4/6)
FokI 867 GGATG GGATG(9/13)
HindIII 95 AAGCTT A^AGCTT
HphI 440 TCACC GGTGA(8/7)
MamI 327 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 160 GGATCC [GA]^GATC[CT]
MseI 785 TTAA T^TAA
NciI 915 CCGGG CC^[GC]GG
NspI 897 GCATGT [GA]CATG^[CT]
PinAI 198 ACCGGT A^CCGGT
PshAI 546 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 528 CAGCTG CAG^CTG
RsaI 50 GTAC GT^AC
SalI 202 GTCGAC G^TCGAC
SfaNI 332 GCATC GCATC(5/9)
SmlI 92 CTCAAG C^T[CT][GA]AG
StuI 429 AGGCCT AGG^CCT
TaqI 203 TCGA T^CGA
Tru9I 785 TTAA T^TAA
TthHB8I 203 TCGA T^CGA
XcmI 267 CCATATCAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 160 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 121 GAATTT [GA]^AATT[CT]
769 GAATTC
AlwNI 296 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
364 CAGTGCCTG
ApoI 121 GAATTT [GA]^AATT[CT]
769 GAATTC
AvaII 40 GGACC G^G[AT]CC
655 GGACC
BbvI 358 GCAGC GCAGC(8/12)
805 GCTGC
BmyI 315 GTGCAC G[AGT]GC[ACT]^C
706 GGGCCC
BpmI 887 CTGGAG CTGGAG(16/14)
947 CTGGAG
BseRI 148 CTCCTC GAGGAG(10/8)
922 GAGGAG
BsmAI 862 GAGAC GTCTC(1/5)
476 GTCTC
Bsp1286I 315 GTGCAC G[AGT]GC[ACT]^C
706 GGGCCC
Cac8I 526 GCCAGC GC[ACGT]^[ACGT]GC
628 GCCAGC
DdeI 151 CTCAG C^T[ACGT]AG
235 CTCAG
Fnu4HI 358 GCAGC GC^[ACGT]GC
805 GCTGC
HapII 199 CCGG C^CGG
915 CCGG
HincII 202 GTCGAC GT[CT]^[GA]AC
910 GTTGAC
HindII 202 GTCGAC GT[CT]^[GA]AC
910 GTTGAC
HpaII 199 CCGG C^CGG
915 CCGG
ItaI 358 GCAGC GC^[ACGT]GC
805 GCTGC
MaeII 777 ACGT A^CGT
908 ACGT
MslI 63 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
831 CATGGAGGTG
MspI 199 CCGG C^CGG
915 CCGG
PflMI 167 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
363 CCAGTGCCTGG
PstI 293 CTGCAG CTGCA^G
659 CTGCAG
SfcI 293 CTGCAG C^T[GA][CT]AG
659 CTGCAG
TseI 358 GCAGC G^C[AT]GC
805 GCTGC
Van91I 167 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
363 CCAGTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI BalI BbsI
BpuAI BsaJI BsiYI BslI BsrI
BssKI BstNI Cfr13I DpnII DsaI
EaeI EcoRII EcoT14I HaeIII HinfI
MaeIII MboI MboII MfeI MluNI
MnlI MscI MspA1I MunI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI StyI
TfiI Tsp45I Tsp509I TspRI