Restriction map for
pFN21AE4415
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI TfiI
Tsp509I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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ApaI 453 GGGCCC GGGCC^C
AspI 933 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1115 TGGCCA TGG^CCA
BamHI 989 GGATCC G^GATCC
BanI 1087 GGCACC G^G[CT][GA]CC
BspHI 917 TCATGA T^CATGA
BstEII 694 GGTTACC G^GT[ACGT]ACC
BstPI 694 GGTTACC G^GT[ACGT]ACC
BstXI 9 CCAGGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 495 GCGC GCG^C
EcoO65I 694 GGTTACC G^GT[ACGT]ACC
FauI 871 GCGGG CCCGC(4/6)
HhaI 495 GCGC GCG^C
HinP1I 495 GCGC G^CGC
HphI 155 TCACC GGTGA(8/7)
MluNI 1115 TGGCCA TGG^CCA
MscI 1115 TGGCCA TGG^CCA
MseI 326 TTAA T^TAA
PflFI 933 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 368 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 649 CTGCAG CTGCA^G
RcaI 917 TCATGA T^CATGA
SacI 288 GAGCTC GAGCT^C
SapI 234 GCTCTTC GCTCTTC(1/4)
SbfI 648 CCTGCAGG CCTGCA^GG
SfaNI 830 GCATC GCATC(5/9)
Sse8387I 648 CCTGCAGG CCTGCA^GG
Tru9I 326 TTAA T^TAA
Tth111I 933 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 428 CCAGCATGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
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AvaII 369 GGTCC G^G[AT]CC
597 GGACC
BcnI 855 CCGGG CC^[GC]GG
954 CCGGG
BglI 818 GCCTTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1216 GCCAGCACGGC
BpmI 285 CTGGAG CTGGAG(16/14)
443 CTGGAG
BseRI 1020 CTCCTC GAGGAG(10/8)
637 GAGGAG
BsmAI 535 GTCTC GTCTC(1/5)
548 GTCTC
BspMI 570 ACCTGC ACCTGC(4/8)
721 ACCTGC
BsrDI 1035 CATTGC GCAATG(2/0)
1158 CATTGC
BstAPI 172 GCAGCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
651 GCAGGAGCTGC
BstYI 122 AGATCC [GA]^GATC[CT]
989 GGATCC
DraIII 405 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
521 CACTGTGTG
DsaI 860 CCATGG C^C[GA][CT]GG
900 CCATGG
EaeI 1115 TGGCCA [CT]^GGCC[GA]
1214 CGGCCA
EcoNI 333 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
750 CCTTATCCAGG
EcoT14I 860 CCATGG C^C[AT][AT]GG
900 CCATGG
HapII 855 CCGG C^CGG
954 CCGG
HincII 268 GTCAAC GT[CT]^[GA]AC
423 GTCAAC
HindII 268 GTCAAC GT[CT]^[GA]AC
423 GTCAAC
HpaII 855 CCGG C^CGG
954 CCGG
MflI 122 AGATCC [GA]^GATC[CT]
989 GGATCC
MslI 930 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1254 CACCAAGATG
MspA1I 489 CAGCTG C[AC]G^C[GT]G
836 CAGCTG
MspI 855 CCGG C^CGG
954 CCGG
NciI 855 CCGGG CC^[GC]GG
954 CCGGG
NcoI 860 CCATGG C^CATGG
900 CCATGG
PflMI 339 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
980 CCAAGCTCTGG
PvuII 489 CAGCTG CAG^CTG
836 CAGCTG
StyI 860 CCATGG C^C[AT][AT]GG
900 CCATGG
Tsp45I 964 GTGAC ^GT[GC]AC
971 GTGAC
Van91I 339 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
980 CCAAGCTCTGG
XhoII 122 AGATCC [GA]^GATC[CT]
989 GGATCC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BanII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfcI TseI TspRI