Restriction map for
pFN21AE3968
##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbrPI BcgI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspEI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CpoI DraI DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 781 GTCTAC GT^[AC][GT]AC
AcyI 1063 GGCGCC G[GA]^CG[CT]C
BamHI 383 GGATCC G^GATCC
BanI 1063 GGCGCC G^G[CT][GA]CC
BbeI 1063 GGCGCC GGCGC^C
BbsI 579 GAAGAC GAAGAC(2/6)
BbvCI 204 CCTCAGC CCTCAGC(-5/-2)
BclI 92 TGATCA T^GATCA
BglI 979 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 579 GAAGAC GAAGAC(2/6)
BsaHI 1063 GGCGCC G[GA]^CG[CT]C
BsgI 102 GTGCAG GTGCAG(16/14)
BsmAI 1089 GAGAC GTCTC(1/5)
BsmI 515 GAATGC GAATGC(1/-1)
BspHI 1100 TCATGA T^CATGA
BsrFI 857 ACCGGC [GA]^CCGG[CT]
BssSI 678 CACGAG CACGAG(-5/-1)
Cfr10I 857 ACCGGC [GA]^CCGG[CT]
DraIII 96 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1155 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 92 TGATCA T^GATCA
Hin1I 1063 GGCGCC G[GA]^CG[CT]C
KasI 1063 GGCGCC G^GCGCC
MaeII 68 ACGT A^CGT
MboII 579 GAAGA GAAGA(8/7)
MseI 1049 TTAA T^TAA
MslI 510 CACCGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1063 GGCGCC GG^CGCC
NcoI 26 CCATGG C^CATGG
NspI 410 GCATGT [GA]CATG^[CT]
PflMI 1149 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1100 TCATGA T^CATGA
SfoI 1063 GGCGCC GGC^GCC
SmaI 362 CCCGGG CCC^GGG
Tru9I 1049 TTAA T^TAA
Tsp509I 178 AATT ^AATT
Van91I 1149 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 23 CCACCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 362 CCCGGG C^CCGGG
XmaI 362 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 546 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1150 CAGCGCCTG
AvaI 362 CCCGGG C^[CT]CG[GA]G
697 CCCGAG
BsaWI 511 ACCGGA [AT]^CCGG[AT]
1085 ACCGGA
BsoBI 362 CCCGGG C^[CT]CG[GA]G
697 CCCGAG
BspDI 151 ATCGAT AT^CGAT
595 ATCGAT
BspMI 104 GCAGGT ACCTGC(4/8)
235 GCAGGT
BsrBI 715 CCGCTC CCGCTC(-3/-3)
655 GAGCGG
BstF5I 504 CATCC GGATG(2/0)
492 GGATG
BstXI 26 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
241 CCAGTGAGCTGG
BstYI 200 AGATCC [GA]^GATC[CT]
383 GGATCC
ClaI 151 ATCGAT AT^CGAT
595 ATCGAT
DraII 839 AGGACCT [GA]G^G[ACGT]CC[CT]
1188 GGGTCCC
EcoO109I 839 AGGACCT [GA]G^G[ACGT]CC[CT]
1188 GGGTCCC
FokI 504 CATCC GGATG(9/13)
492 GGATG
HgaI 55 GCGTC GACGC(5/10)
816 GCGTC
MflI 200 AGATCC [GA]^GATC[CT]
383 GGATCC
PpuMI 839 AGGACCT [GA]G^G[AT]CC[CT]
1188 GGGTCCC
SacI 1003 GAGCTC GAGCT^C
1137 GAGCTC
SfaNI 332 GCATC GCATC(5/9)
776 GCATC
XhoII 200 AGATCC [GA]^GATC[CT]
383 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I