Restriction map for
pFN21AE2722
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbsI BcgI BciVI BclI BfrI
BglI BlnI BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AatII 161 GACGTC GACGT^C
AlwNI 463 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 570 AGCGCT AGC^GCT
ApaI 179 GGGCCC GGGCC^C
Asp700I 710 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 179 GGGCCC G[GA]GC[CT]^C
BbvCI 289 GCTGAGG CCTCAGC(-5/-2)
BglII 407 AGATCT A^GATCT
BlpI 456 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC
BseRI 855 GAGGAG GAGGAG(10/8)
BstAPI 266 GCATTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 314 CCAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 456 GCTGAGC GC^T[ACGT]AGC
EarI 510 CTCTTC CTCTTC(1/4)
Eco47III 570 AGCGCT AGC^GCT
FauI 262 GCGGG CCCGC(4/6)
HphI 675 GGTGA GGTGA(8/7)
Ksp632I 510 CTCTTC CTCTTC(1/4)
MaeIII 121 GTCAC ^GT[ACGT]AC
NcoI 227 CCATGG C^CATGG
SalI 340 GTCGAC G^TCGAC
SfaNI 795 GATGC GCATC(5/9)
TaqI 341 TCGA T^CGA
TfiI 769 GAATC G^A[AT]TC
Tsp45I 121 GTCAC ^GT[GC]AC
Tsp509I 648 AATT ^AATT
TthHB8I 341 TCGA T^CGA
XmnI 710 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
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AccI 340 GTCGAC GT^[AC][GT]AC
550 GTCTAC
AlwI 420 GATCC GGATC(4/5)
140 GGATC
AvaI 17 CCCGGG C^[CT]CG[GA]G
170 CCCGGG
BalI 230 TGGCCA TGG^CCA
311 TGGCCA
BbrPI 184 CACGTG CAC^GTG
277 CACGTG
BfaI 50 CTAG C^TAG
587 CTAG
BpmI 790 CTCCAG CTGGAG(16/14)
322 CTGGAG
BsaAI 184 CACGTG [CT]AC^GT[GA]
277 CACGTG
BsaI 507 GGTCTC GGTCTC(1/5)
580 GGTCTC
BsgI 354 GTGCAG GTGCAG(16/14)
763 GTGCAG
BsoBI 17 CCCGGG C^[CT]CG[GA]G
170 CCCGGG
BsrDI 516 CATTGC GCAATG(2/0)
821 CATTGC
BstYI 407 AGATCT [GA]^GATC[CT]
419 AGATCC
DraII 178 GGGGCCC [GA]G^G[ACGT]CC[CT]
317 AGGCCCT
DsaI 116 CCGTGG C^C[GA][CT]GG
227 CCATGG
EaeI 230 TGGCCA [CT]^GGCC[GA]
311 TGGCCA
EcoNI 44 CCTGTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
373 CCTCTGGTAGG
EcoO109I 178 GGGGCCC [GA]G^G[ACGT]CC[CT]
317 AGGCCCT
HaeII 540 GGCGCT [GA]GCGC^[CT]
570 AGCGCT
HgaI 95 GCGTC GACGC(5/10)
338 GCGTC
MaeI 50 CTAG C^TAG
587 CTAG
MboII 710 GAAGA GAAGA(8/7)
511 TCTTC
MflI 407 AGATCT [GA]^GATC[CT]
419 AGATCC
MluNI 230 TGGCCA TGG^CCA
311 TGGCCA
MscI 230 TGGCCA TGG^CCA
311 TGGCCA
MspA1I 304 CAGCTG C[AC]G^C[GT]G
732 CAGCTG
MwoI 23 GCTACCAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
266 GCATTGTGTGC
PleI 699 GAGTC GAGTC(4/5)
750 GAGTC
PmaCI 184 CACGTG CAC^GTG
277 CACGTG
PmlI 184 CACGTG CAC^GTG
277 CACGTG
PstI 238 CTGCAG CTGCA^G
735 CTGCAG
PvuII 304 CAGCTG CAG^CTG
732 CAGCTG
SfcI 238 CTGCAG C^T[GA][CT]AG
735 CTGCAG
SmaI 17 CCCGGG CCC^GGG
170 CCCGGG
XhoII 407 AGATCT [GA]^GATC[CT]
419 AGATCC
XmaCI 17 CCCGGG C^CCGGG
170 CCCGGG
XmaI 17 CCCGGG C^CCGGG
170 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcyI AluI AspHI AvaII
BbvI BcnI BmyI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII ItaI MaeII
MboI MnlI MslI MspI MvaI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI StyI TseI TspRI