Restriction map for
pFN21AE6634
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstXI BstZ17I
CelII CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MslI MunI NarI NciI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 257 CGCG CG^CG
ApaI 389 GGGCCC GGGCC^C
AspHI 441 GAGCAC G[AT]GC[AT]^C
AvaII 273 GGACC G^G[AT]CC
BalI 218 TGGCCA TGG^CCA
BanII 389 GGGCCC G[GA]GC[CT]^C
BbvCI 639 GCTGAGG CCTCAGC(-5/-2)
BcgI 37 CGAAGCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 235 CTAG C^TAG
BpmI 424 CTGGAG CTGGAG(16/14)
BsiHKAI 441 GAGCAC G[AT]GC[AT]^C
BsmFI 248 GTCCC GGGAC(10/14)
BspMI 171 GCAGGT ACCTGC(4/8)
BsrFI 614 GCCGGC [GA]^CCGG[CT]
BstBI 59 TTCGAA TT^CGAA
BstEII 474 GGTGACC G^GT[ACGT]ACC
BstPI 474 GGTGACC G^GT[ACGT]ACC
BstUI 257 CGCG CG^CG
Cfr10I 614 GCCGGC [GA]^CCGG[CT]
DraII 272 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 479 CCATGG C^C[GA][CT]GG
EaeI 218 TGGCCA [CT]^GGCC[GA]
EarI 282 GAAGAG CTCTTC(1/4)
EcoO109I 272 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 474 GGTGACC G^GT[ACGT]ACC
EcoT14I 479 CCATGG C^C[AT][AT]GG
HapII 615 CCGG C^CGG
HgaI 246 GCGTC GACGC(5/10)
HpaII 615 CCGG C^CGG
Ksp632I 282 GAAGAG CTCTTC(1/4)
MaeI 235 CTAG C^TAG
MluNI 218 TGGCCA TGG^CCA
MscI 218 TGGCCA TGG^CCA
MseI 416 TTAA T^TAA
MspI 615 CCGG C^CGG
MvnI 257 CGCG CG^CG
NaeI 614 GCCGGC GCC^GGC
NcoI 479 CCATGG C^CATGG
NgoMIV 614 GCCGGC G^CCGGC
NspV 59 TTCGAA TT^CGAA
PflMI 210 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 272 AGGACCC [GA]G^G[AT]CC[CT]
PstI 337 CTGCAG CTGCA^G
PvuII 379 CAGCTG CAG^CTG
SfuI 59 TTCGAA TT^CGAA
StuI 590 AGGCCT AGG^CCT
StyI 479 CCATGG C^C[AT][AT]GG
Tru9I 416 TTAA T^TAA
Tsp509I 63 AATT ^AATT
Van91I 210 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 234 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 432 GATCC GGATC(4/5)
534 GGATC
AocI 268 CCTCAGG CC^T[ACGT]AGG
502 CCTCAGG
BseRI 238 GAGGAG GAGGAG(10/8)
370 GAGGAG
BsiYI 44 CCTGCTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
210 CCAGTCACTGG
BslI 44 CCTGCTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
210 CCAGTCACTGG
BsmBI 94 GAGACG CGTCTC(1/5)
467 GAGACG
BsrI 216 ACTGG ACTGG(1/-1)
210 CCAGT
BstYI 431 AGATCC [GA]^GATC[CT]
534 GGATCT
Bsu36I 268 CCTCAGG CC^T[ACGT]AGG
502 CCTCAGG
Eco81I 268 CCTCAGG CC^T[ACGT]AGG
502 CCTCAGG
FauI 7 GCGGG CCCGC(4/6)
448 GCGGG
MaeIII 213 GTCAC ^GT[ACGT]AC
475 GTGAC
MboII 186 GAAGA GAAGA(8/7)
282 GAAGA
MflI 431 AGATCC [GA]^GATC[CT]
534 GGATCT
MspA1I 379 CAGCTG C[AC]G^C[GT]G
446 CAGCGG
NlaIII 50 CATG CATG^
480 CATG
SfcI 337 CTGCAG C^T[GA][CT]AG
436 CTACAG
TaqI 60 TCGA T^CGA
86 TCGA
Tsp45I 213 GTCAC ^GT[GC]AC
475 GTGAC
TthHB8I 60 TCGA T^CGA
86 TCGA
XhoII 431 AGATCC [GA]^GATC[CT]
534 GGATCT
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AlwNI BanI BbvI
BmyI BsaJI BsmAI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MboI MnlI
MvaI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
TspRI