Restriction map for
pFN21AE2105
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I FbaI FseI FspI
HaeII HgaI Hin1I HindIII KasI
KpnI Ksp632I KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 14 CGCG CG^CG
AocI 195 CCTGAGG CC^T[ACGT]AGG
AspHI 532 GAGCAC G[AT]GC[AT]^C
AspI 2395 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2147 TGGCCA TGG^CCA
BanI 2097 GGTGCC G^G[CT][GA]CC
BanII 79 GAGCCC G[GA]GC[CT]^C
BciVI 1605 GGATAC GTATCC(6/5)
BcnI 2140 CCCGG CC^[GC]GG
BsaI 63 GAGACC GGTCTC(1/5)
BsiHKAI 532 GAGCAC G[AT]GC[AT]^C
BsmBI 2424 GAGACG CGTCTC(1/5)
BstAPI 1845 GCAGGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 14 CGCG CG^CG
Bsu36I 195 CCTGAGG CC^T[ACGT]AGG
CfoI 15 GCGC GCG^C
DraII 833 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 41 CCATGG C^C[GA][CT]GG
EaeI 2147 TGGCCA [CT]^GGCC[GA]
Eco81I 195 CCTGAGG CC^T[ACGT]AGG
EcoNI 2310 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 833 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 859 GAATTC G^AATTC
EcoRV 1468 GATATC GAT^ATC
EcoT14I 41 CCATGG C^C[AT][AT]GG
EcoT22I 117 ATGCAT ATGCA^T
FauI 585 GCGGG CCCGC(4/6)
HapII 2141 CCGG C^CGG
HhaI 15 GCGC GCG^C
HinP1I 15 GCGC G^CGC
HpaI 1012 GTTAAC GTT^AAC
HpaII 2141 CCGG C^CGG
MaeII 1609 ACGT A^CGT
MaeIII 952 GTGAC ^GT[ACGT]AC
MluNI 2147 TGGCCA TGG^CCA
MscI 2147 TGGCCA TGG^CCA
MspA1I 2232 CCGCTG C[AC]G^C[GT]G
MspI 2141 CCGG C^CGG
MvnI 14 CGCG CG^CG
NciI 2140 CCCGG CC^[GC]GG
NcoI 41 CCATGG C^CATGG
NsiI 117 ATGCAT ATGCA^T
NspI 1850 GCATGC [GA]CATG^[CT]
PflFI 2395 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 765 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 657 ACCAGGT A^CC[AT]GGT
SphI 1850 GCATGC GCATG^C
StyI 41 CCATGG C^C[AT][AT]GG
Tsp45I 952 GTGAC ^GT[GC]AC
Tth111I 2395 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 765 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 33 GATCC GGATC(4/5)
1778 GGATC
BbsI 2325 GAAGAC GAAGAC(2/6)
1562 GTCTTC
BglII 1134 AGATCT A^GATCT
1212 AGATCT
BmyI 79 GAGCCC G[AGT]GC[ACT]^C
532 GAGCAC
BpuAI 2325 GAAGAC GAAGAC(2/6)
1562 GTCTTC
BsmI 1092 GAATGC GAATGC(1/-1)
1290 GCATTC
Bsp1286I 79 GAGCCC G[AGT]GC[ACT]^C
532 GAGCAC
BspMI 240 GCAGGT ACCTGC(4/8)
2343 GCAGGT
BsrDI 2151 CATTGC GCAATG(2/0)
1773 GCAATG
BstXI 67 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
167 CCAAGAACCTGG
BstYI 1134 AGATCT [GA]^GATC[CT]
1212 AGATCT
DraI 667 TTTAAA TTT^AAA
717 TTTAAA
HincII 1012 GTTAAC GT[CT]^[GA]AC
1517 GTCAAC
HindII 1012 GTTAAC GT[CT]^[GA]AC
1517 GTCAAC
MfeI 275 CAATTG C^AATTG
2493 CAATTG
MflI 1134 AGATCT [GA]^GATC[CT]
1212 AGATCT
MslI 1851 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1899 CATTGGTGTG
MunI 275 CAATTG C^AATTG
2493 CAATTG
PleI 27 GACTC GAGTC(4/5)
2429 GACTC
PstI 238 CTGCAG CTGCA^G
2311 CTGCAG
SspI 103 AATATT AAT^ATT
321 AATATT
TaqI 890 TCGA T^CGA
1418 TCGA
TfiI 558 GATTC G^A[AT]TC
2463 GAATC
TthHB8I 890 TCGA T^CGA
1418 TCGA
XhoII 1134 AGATCT [GA]^GATC[CT]
1212 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AvaII BbvI BfaI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI Tru9I TseI Tsp509I
TspRI XbaI