Restriction map for
pFN21AE2314
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Aor51HI ApaI AscI Asp718I AspEI
AvrII BalI BamHI BbrPI BcgI
BglI BlnI BsiEI BsmBI BsrFI
BssHII Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I Cfr10I CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoT22I FseI HgaI
KpnI MluI MluNI MscI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SalI SbfI SfiI
SgrAI SnaBI SphI Sse8387I XcmI
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 79 TCCGGA T^CCGGA
AflII 4072 CTTAAG C^TTAAG
Alw44I 4970 GTGCAC G^TGCAC
ApaLI 4970 GTGCAC G^TGCAC
AseI 2734 ATTAAT AT^TAAT
AsnI 2734 ATTAAT AT^TAAT
Asp700I 5631 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 4970 GTGCAC G[AT]GC[AT]^C
BbvCI 6055 GCTGAGG CCTCAGC(-5/-2)
BfrI 4072 CTTAAG C^TTAAG
BglII 6062 AGATCT A^GATCT
BseAI 79 TCCGGA T^CCGGA
BsiHKAI 4970 GTGCAC G[AT]GC[AT]^C
BsiWI 182 CGTACG C^GTACG
BspDI 2258 ATCGAT AT^CGAT
BspEI 79 TCCGGA T^CCGGA
BspLU11I 2725 ACATGT A^CATGT
BsrDI 2111 CATTGC GCAATG(2/0)
BssSI 3608 CTCGTG CACGAG(-5/-1)
ClaI 2258 ATCGAT AT^CGAT
DraII 1850 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1850 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 973 GATATC GAT^ATC
HincII 5068 GTTAAC GT[CT]^[GA]AC
HindII 5068 GTTAAC GT[CT]^[GA]AC
HpaI 5068 GTTAAC GTT^AAC
KspI 233 CCGCGG CCGC^GG
MfeI 261 CAATTG C^AATTG
MroI 79 TCCGGA T^CCGGA
MunI 261 CAATTG C^AATTG
PpuMI 1850 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1518 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2734 ATTAAT AT^TAAT
PstI 5659 CTGCAG CTGCA^G
SacII 233 CCGCGG CCGC^GG
ScaI 1273 AGTACT AGT^ACT
SmaI 296 CCCGGG CCC^GGG
StuI 2277 AGGCCT AGG^CCT
SwaI 2052 ATTTAAAT ATTT^AAAT
XmaCI 296 CCCGGG C^CCGGG
XmaI 296 CCCGGG C^CCGGG
XmnI 5631 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 510 GTCTAC GT^[AC][GT]AC
5271 GTCTAC
AccII 177 CGCG CG^CG
234 CGCG
AcyI 117 GGCGCC G[GA]^CG[CT]C
238 GGCGCC
AflIII 1267 ACACGT A^C[GA][CT]GT
2725 ACATGT
AvaI 296 CCCGGG C^[CT]CG[GA]G
1430 CCCGAG
AviII 5139 TGCGCA TGC^GCA
5433 TGCGCA
BanI 117 GGCGCC G^G[CT][GA]CC
238 GGCGCC
BbeI 117 GGCGCC GGCGC^C
238 GGCGCC
BciVI 5217 GGATAC GTATCC(6/5)
476 GTATCC
BlpI 142 GCTCAGC GC^T[ACGT]AGC
190 GCTGAGC
Bpu1102I 142 GCTCAGC GC^T[ACGT]AGC
190 GCTGAGC
BsaAI 1262 CACGTA [CT]AC^GT[GA]
1268 CACGTA
BsaBI 471 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2975 GATTTTTATC
BsaHI 117 GGCGCC G[GA]^CG[CT]C
238 GGCGCC
BsaWI 79 TCCGGA [AT]^CCGG[AT]
337 ACCGGA
BsoBI 296 CCCGGG C^[CT]CG[GA]G
1430 CCCGAG
BsrBI 3 GAGCGG CCGCTC(-3/-3)
193 GAGCGG
BstBI 2887 TTCGAA TT^CGAA
3825 TTCGAA
BstUI 177 CGCG CG^CG
234 CGCG
CelII 142 GCTCAGC GC^T[ACGT]AGC
190 GCTGAGC
DraIII 1928 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3932 CACTGAGTG
DsaI 233 CCGCGG C^C[GA][CT]GG
365 CCGTGG
FspI 5139 TGCGCA TGC^GCA
5433 TGCGCA
Hin1I 117 GGCGCC G[GA]^CG[CT]C
238 GGCGCC
HindIII 518 AAGCTT A^AGCTT
862 AAGCTT
KasI 117 GGCGCC G^GCGCC
238 GGCGCC
MamI 471 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2975 GATTTTTATC
MvnI 177 CGCG CG^CG
234 CGCG
NarI 117 GGCGCC GG^CGCC
238 GGCGCC
NspI 2725 ACATGT [GA]CATG^[CT]
4515 GCATGT
NspV 2887 TTCGAA TT^CGAA
3825 TTCGAA
PflMI 465 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
915 CCAGATTTTGG
SapI 1692 GAAGAGC GCTCTTC(1/4)
2893 GAAGAGC
SexAI 464 ACCAGGT A^CC[AT]GGT
741 ACCAGGT
SfoI 117 GGCGCC GGC^GCC
238 GGCGCC
SfuI 2887 TTCGAA TT^CGAA
3825 TTCGAA
Van91I 465 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
915 CCAGATTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspI AvaII
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspMI BsrGI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI Eco81I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XbaI XhoII