Restriction map for
pFN21AE2312
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# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
AscI AseI AsnI Asp718I AspI
AviII BbvCI BclI BfrI BglI
BsiEI BsiWI BspDI BssHII BstBI
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I EcoRI FbaI FseI
FspI HpaI KpnI KspI MfeI
MunI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 5982 CGCG CG^CG
AccIII 3395 TCCGGA T^CCGGA
AhdI 5347 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4046 GGGCCC GGGCC^C
AspEI 5347 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5442 CCTAGG C^CTAGG
BbeI 5430 GGCGCC GGCGC^C
BbrPI 383 CACGTG CAC^GTG
BciVI 4992 GGATAC GTATCC(6/5)
BlnI 5442 CCTAGG C^CTAGG
BsaBI 1486 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3395 TCCGGA T^CCGGA
BspEI 3395 TCCGGA T^CCGGA
BspLU11I 2219 ACATGT A^CATGT
BsrBI 4444 GAGCGG CCGCTC(-3/-3)
BssSI 2386 CTCGTG CACGAG(-5/-1)
BstEII 5844 GGTCACC G^GT[ACGT]ACC
BstPI 5844 GGTCACC G^GT[ACGT]ACC
BstUI 5982 CGCG CG^CG
DraIII 1010 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5347 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5844 GGTCACC G^GT[ACGT]ACC
EcoRV 388 GATATC GAT^ATC
KasI 5430 GGCGCC G^GCGCC
MamI 1486 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 5981 ACGCGT A^CGCGT
MroI 3395 TCCGGA T^CCGGA
MvnI 5982 CGCG CG^CG
NarI 5430 GGCGCC GG^CGCC
NdeI 2063 CATATG CA^TATG
PmaCI 383 CACGTG CAC^GTG
PmlI 383 CACGTG CAC^GTG
PshAI 1867 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 5430 GGCGCC GGC^GCC
StuI 5771 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 32 enzymes) #
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AclI 3178 AACGTT AA^CGTT
5915 AACGTT
AcyI 4886 GACGCC G[GA]^CG[CT]C
5430 GGCGCC
Alw44I 1007 GTGCAC G^TGCAC
3562 GTGCAC
ApaLI 1007 GTGCAC G^TGCAC
3562 GTGCAC
Asp700I 3435 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5130 GAAGGATTTC
BamHI 1297 GGATCC G^GATCC
1448 GGATCC
BglII 1167 AGATCT A^GATCT
2862 AGATCT
BsaAI 383 CACGTG [CT]AC^GT[GA]
1994 TACGTG
BsaHI 4886 GACGCC G[GA]^CG[CT]C
5430 GGCGCC
BsaWI 86 ACCGGA [AT]^CCGG[AT]
3395 TCCGGA
BsmBI 4691 CGTCTC CGTCTC(1/5)
1480 GAGACG
Bsp1407I 2498 TGTACA T^GTACA
4130 TGTACA
BsrFI 230 ACCGGC [GA]^CCGG[CT]
3266 GCCGGT
BsrGI 2498 TGTACA T^GTACA
4130 TGTACA
BstXI 1589 CCATCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4490 CCAAGGATGTGG
Cfr10I 230 ACCGGC [GA]^CCGG[CT]
3266 GCCGGT
DrdI 694 GACGAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1940 GACTCATTGGTC
EcoT22I 791 ATGCAT ATGCA^T
5194 ATGCAT
Hin1I 4886 GACGCC G[GA]^CG[CT]C
5430 GGCGCC
HincII 1999 GTTGAC GT[CT]^[GA]AC
2374 GTTGAC
HindII 1999 GTTGAC GT[CT]^[GA]AC
2374 GTTGAC
HindIII 1886 AAGCTT A^AGCTT
2686 AAGCTT
MseI 953 TTAA T^TAA
4007 TTAA
NsiI 791 ATGCAT ATGCA^T
5194 ATGCAT
Psp1406I 3178 AACGTT AA^CGTT
5915 AACGTT
SacI 4110 GAGCTC GAGCT^C
5488 GAGCTC
SbfI 5316 CCTGCAGG CCTGCA^GG
5682 CCTGCAGG
SexAI 1238 ACCTGGT A^CC[AT]GGT
4976 ACCTGGT
Sse8387I 5316 CCTGCAGG CCTGCA^GG
5682 CCTGCAGG
SspBI 2498 TGTACA T^GTACA
4130 TGTACA
Tru9I 953 TTAA T^TAA
4007 TTAA
XmnI 3435 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5130 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI Bst1107I BstAPI
BstF5I BstNI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII