Restriction map for
pFN21AB9894
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# NO CUT ENZYMES ( 40 enzymes) #
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AatII AgeI AhdI Aor51HI AscI
AspEI BbeI BbrPI BsiWI BssHII
BstEII BstPI CpoI DrdI Eam1105I
Eco47III EcoO65I FseI KasI MluI
NarI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PvuI
RsrII SalI SbfI SfiI SfoI
SgrAI SnaBI Sse8387I SwaI XhoI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AclI 8237 AACGTT AA^CGTT
AcyI 5123 GGCGTC G[GA]^CG[CT]C
ApaI 4438 GGGCCC GGGCC^C
AviII 5864 TGCGCA TGC^GCA
AvrII 5305 CCTAGG C^CTAGG
BlnI 5305 CCTAGG C^CTAGG
BsaAI 7789 TACGTG [CT]AC^GT[GA]
BsaHI 5123 GGCGTC G[GA]^CG[CT]C
BsiEI 5035 CGGCCG CG[GA][CT]^CG
BsmBI 5934 GAGACG CGTCTC(1/5)
BsrBI 8433 GAGCGG CCGCTC(-3/-3)
EagI 5035 CGGCCG C^GGCCG
EclXI 5035 CGGCCG C^GGCCG
Eco52I 5035 CGGCCG C^GGCCG
FspI 5864 TGCGCA TGC^GCA
Hin1I 5123 GGCGTC G[GA]^CG[CT]C
HpaI 328 GTTAAC GTT^AAC
KspI 64 CCGCGG CCGC^GG
NaeI 5037 GCCGGC GCC^GGC
NgoMIV 5037 GCCGGC G^CCGGC
NheI 9747 GCTAGC G^CTAGC
Psp1406I 8237 AACGTT AA^CGTT
SacII 64 CCGCGG CCGC^GG
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# TWO CUT ENZYMES ( 41 enzymes) #
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AccII 65 CGCG CG^CG
3083 CGCG
AccIII 3837 TCCGGA T^CCGGA
10625 TCCGGA
AflII 2152 CTTAAG C^TTAAG
8730 CTTAAG
Alw44I 1945 GTGCAC G^TGCAC
2419 GTGCAC
AocI 1756 CCTGAGG CC^T[ACGT]AGG
3538 CCTGAGG
ApaLI 1945 GTGCAC G^TGCAC
2419 GTGCAC
BclI 2025 TGATCA T^GATCA
2871 TGATCA
BfrI 2152 CTTAAG C^TTAAG
8730 CTTAAG
BglI 3592 GCCGATTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4867 GCCAGCTGGGC
BsaWI 3837 TCCGGA [AT]^CCGG[AT]
10625 TCCGGA
BseAI 3837 TCCGGA T^CCGGA
10625 TCCGGA
BspDI 417 ATCGAT AT^CGAT
8779 ATCGAT
BspEI 3837 TCCGGA T^CCGGA
10625 TCCGGA
BspLU11I 3827 ACATGT A^CATGT
5921 ACATGT
BsrFI 5037 GCCGGC [GA]^CCGG[CT]
9038 ACCGGC
Bst1107I 4194 GTATAC GTA^TAC
8550 GTATAC
BstAPI 4285 GCAAAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
9790 GCAGGTGGTGC
BstUI 65 CGCG CG^CG
3083 CGCG
BstXI 1467 CCAGACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3215 CCATTATGATGG
BstZ17I 4194 GTATAC GTA^TAC
8550 GTATAC
Bsu36I 1756 CCTGAGG CC^T[ACGT]AGG
3538 CCTGAGG
Cfr10I 5037 GCCGGC [GA]^CCGG[CT]
9038 ACCGGC
ClaI 417 ATCGAT AT^CGAT
8779 ATCGAT
Eco81I 1756 CCTGAGG CC^T[ACGT]AGG
3538 CCTGAGG
EcoNI 2166 CCTTAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3186 CCTATCTCAGG
EcoRI 5939 GAATTC G^AATTC
8571 GAATTC
EcoRV 5133 GATATC GAT^ATC
8497 GATATC
EcoT22I 2987 ATGCAT ATGCA^T
3008 ATGCAT
FbaI 2025 TGATCA T^GATCA
2871 TGATCA
HaeII 1896 GGCGCT [GA]GCGC^[CT]
7889 GGCGCT
MroI 3837 TCCGGA T^CCGGA
10625 TCCGGA
MvnI 65 CGCG CG^CG
3083 CGCG
NsiI 2987 ATGCAT ATGCA^T
3008 ATGCAT
PpuMI 1118 AGGACCT [GA]G^G[AT]CC[CT]
1260 GGGTCCT
PshAI 7771 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
10075 GACACTGGTC
SexAI 1331 ACCAGGT A^CC[AT]GGT
8477 ACCTGGT
SmaI 15 CCCGGG CCC^GGG
470 CCCGGG
SpeI 552 ACTAGT A^CTAGT
2415 ACTAGT
SphI 2335 GCATGC GCATG^C
5394 GCATGC
XmaCI 15 CCCGGG C^CCGGG
470 CCCGGG
XmaI 15 CCCGGG C^CCGGG
470 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcgI BciVI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrGI BsrI BssKI
BssSI BstBI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI NspV PflFI PflMI
PleI PshBI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SfuI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XcmI XhoII XmnI