Restriction map for
pFN21AB7977
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 768 AAATTC [GA]^AATT[CT]
Alw44I 678 GTGCAC G^TGCAC
ApaLI 678 GTGCAC G^TGCAC
ApoI 768 AAATTC [GA]^AATT[CT]
AspHI 678 GTGCAC G[AT]GC[AT]^C
AspI 265 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 270 GGTCC G^G[AT]CC
BamHI 9 GGATCC G^GATCC
BcnI 273 CCCGG CC^[GC]GG
BfaI 842 CTAG C^TAG
BmyI 678 GTGCAC G[AGT]GC[ACT]^C
BseRI 155 CTCCTC GAGGAG(10/8)
BsiHKAI 678 GTGCAC G[AT]GC[AT]^C
BsiYI 683 CCCAATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 683 CCCAATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 678 GTGCAC G[AGT]GC[ACT]^C
BsrDI 485 GCAATG GCAATG(2/0)
BstXI 779 CCAGTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 269 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 472 TGGCCG [CT]^GGCC[GA]
EcoO109I 269 AGGTCCC [GA]G^G[ACGT]CC[CT]
HapII 274 CCGG C^CGG
HgaI 495 GACGC GACGC(5/10)
HindIII 27 AAGCTT A^AGCTT
HpaII 274 CCGG C^CGG
MaeI 842 CTAG C^TAG
MaeII 911 ACGT A^CGT
MseI 821 TTAA T^TAA
MslI 891 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 316 CAGCTG C[AC]G^C[GT]G
MspI 274 CCGG C^CGG
NciI 273 CCCGG CC^[GC]GG
PflFI 265 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 269 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 316 CAGCTG CAG^CTG
ScaI 853 AGTACT AGT^ACT
SphI 752 GCATGC GCATG^C
Tru9I 821 TTAA T^TAA
TspRI 780 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 265 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 325 GTAC GT^AC
854 GTAC
BbvI 793 GCTGC GCAGC(8/12)
805 GCTGC
BstYI 9 GGATCC [GA]^GATC[CT]
174 GGATCT
Cac8I 29 GCTTGC GC[ACGT]^[ACGT]GC
752 GCATGC
Cfr13I 88 GGGCC G^G[ACGT]CC
270 GGTCC
DpnII 10 GATC ^GATC
175 GATC
DsaI 170 CCATGG C^C[GA][CT]GG
866 CCATGG
Fnu4HI 793 GCTGC GC^[ACGT]GC
805 GCTGC
ItaI 793 GCTGC GC^[ACGT]GC
805 GCTGC
MaeIII 493 GTGAC ^GT[ACGT]AC
782 GTGAC
MboI 10 GATC ^GATC
175 GATC
MflI 9 GGATCC [GA]^GATC[CT]
174 GGATCT
NcoI 170 CCATGG C^CATGG
866 CCATGG
NdeII 10 GATC ^GATC
175 GATC
NspI 752 GCATGC [GA]CATG^[CT]
896 ACATGC
PstI 236 CTGCAG CTGCA^G
742 CTGCAG
RsaI 325 GTAC GT^AC
854 GTAC
Sau3AI 10 GATC ^GATC
175 GATC
Sau96I 88 GGGCC G^G[ACGT]CC
270 GGTCC
SexAI 421 ACCTGGT A^CC[AT]GGT
673 ACCTGGT
SfcI 236 CTGCAG C^T[GA][CT]AG
742 CTGCAG
TseI 793 GCTGC G^C[AT]GC
805 GCTGC
Tsp45I 493 GTGAC ^GT[GC]AC
782 GTGAC
Tsp509I 607 AATT ^AATT
769 AATT
XhoII 9 GGATCC [GA]^GATC[CT]
174 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI AlwI BbsI BbvCI BpmI
BpuAI BsaJI BsmAI BsmFI BsrI
BssKI BstF5I BstNI DdeI EarI
EcoRII EcoT14I FokI HaeIII HinfI
HphI Ksp632I MboII MnlI MvaI
MwoI NlaIII NlaIV ScrFI SfaNI
StuI StyI TfiI