Restriction map for
pFN21AE5805
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BcgI
BfrI BlnI BsaAI BsaBI BsaHI
BsiWI BspDI BspLU11I Bst1107I BstZ17I
ClaI CpoI DraI Eam1105I Eco47III
EcoT22I FseI FspI Hin1I HpaI
KasI KpnI KspI MamI MluI
NarI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 283 GTCGAC GT^[AC][GT]AC
AocI 49 CCTCAGG CC^T[ACGT]AGG
BamHI 535 GGATCC G^GATCC
BanI 2283 GGCACC G^G[CT][GA]CC
BbsI 1312 GTCTTC GAAGAC(2/6)
BbvCI 1254 CCTCAGC CCTCAGC(-5/-2)
BciVI 551 GGATAC GTATCC(6/5)
BclI 2234 TGATCA T^GATCA
BfaI 2182 CTAG C^TAG
BglII 1781 AGATCT A^GATCT
BpuAI 1312 GTCTTC GAAGAC(2/6)
BsaI 2125 GAGACC GGTCTC(1/5)
BsgI 2518 CTGCAC GTGCAG(16/14)
BsiEI 2359 CGGCCG CG[GA][CT]^CG
Bsp1407I 776 TGTACA T^GTACA
BsrGI 776 TGTACA T^GTACA
BssHII 1000 GCGCGC G^CGCGC
BstAPI 851 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 49 CCTCAGG CC^T[ACGT]AGG
DraIII 1212 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 89 GACAGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2359 CGGCCG C^GGCCG
EclXI 2359 CGGCCG C^GGCCG
Eco52I 2359 CGGCCG C^GGCCG
Eco81I 49 CCTCAGG CC^T[ACGT]AGG
EcoRI 589 GAATTC G^AATTC
EcoRV 2383 GATATC GAT^ATC
FbaI 2234 TGATCA T^GATCA
HindIII 1865 AAGCTT A^AGCTT
MaeI 2182 CTAG C^TAG
MfeI 1160 CAATTG C^AATTG
MunI 1160 CAATTG C^AATTG
NcoI 2647 CCATGG C^CATGG
NspI 254 GCATGC [GA]CATG^[CT]
SalI 283 GTCGAC G^TCGAC
SmaI 116 CCCGGG CCC^GGG
SphI 254 GCATGC GCATG^C
SspBI 776 TGTACA T^GTACA
XmaCI 116 CCCGGG C^CCGGG
XmaI 116 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
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AccIII 548 TCCGGA T^CCGGA
2217 TCCGGA
AcsI 469 GAATTT [GA]^AATT[CT]
589 GAATTC
Alw44I 322 GTGCAC G^TGCAC
832 GTGCAC
AlwNI 568 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
679 CAGAAGCTG
ApaLI 322 GTGCAC G^TGCAC
832 GTGCAC
ApoI 469 GAATTT [GA]^AATT[CT]
589 GAATTC
Asp700I 1168 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1203 GAATGCCTTC
BseAI 548 TCCGGA T^CCGGA
2217 TCCGGA
BsmBI 363 CGTCTC CGTCTC(1/5)
2355 GAGACG
BsmI 1203 GAATGC GAATGC(1/-1)
2021 GCATTC
BspEI 548 TCCGGA T^CCGGA
2217 TCCGGA
BspHI 933 TCATGA T^CATGA
1619 TCATGA
BsrBI 427 CCGCTC CCGCTC(-3/-3)
1682 CCGCTC
BsrDI 651 CATTGC GCAATG(2/0)
2451 CATTGC
BsrFI 38 GCCGGC [GA]^CCGG[CT]
2877 GCCGGC
BstEII 1588 GGTTACC G^GT[ACGT]ACC
1972 GGTTACC
BstPI 1588 GGTTACC G^GT[ACGT]ACC
1972 GGTTACC
BstXI 1346 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2167 CCAGAGCAGTGG
Cfr10I 38 GCCGGC [GA]^CCGG[CT]
2877 GCCGGC
EcoO65I 1588 GGTTACC G^GT[ACGT]ACC
1972 GGTTACC
HaeII 1789 GGCGCT [GA]GCGC^[CT]
1850 AGCGCC
HgaI 2577 GACGC GACGC(5/10)
2750 GCGTC
HincII 283 GTCGAC GT[CT]^[GA]AC
2530 GTCAAC
HindII 283 GTCGAC GT[CT]^[GA]AC
2530 GTCAAC
MroI 548 TCCGGA T^CCGGA
2217 TCCGGA
MseI 887 TTAA T^TAA
2627 TTAA
NaeI 38 GCCGGC GCC^GGC
2877 GCCGGC
NgoMIV 38 GCCGGC G^CCGGC
2877 GCCGGC
PvuII 2563 CAGCTG CAG^CTG
2953 CAGCTG
RcaI 933 TCATGA T^CATGA
1619 TCATGA
SacI 687 GAGCTC GAGCT^C
2014 GAGCTC
SapI 1846 GAAGAGC GCTCTTC(1/4)
2814 GAAGAGC
ScaI 2492 AGTACT AGT^ACT
2681 AGTACT
StuI 1447 AGGCCT AGG^CCT
1942 AGGCCT
TfiI 2390 GAATC G^A[AT]TC
2769 GATTC
Tru9I 887 TTAA T^TAA
2627 TTAA
XmnI 1168 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1203 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BalI
BanII BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstBI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspV PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfuI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII