Restriction map for
pFN21AE3949
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BbsI BcgI
BciVI BclI BglII BlnI BpuAI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AflII 1546 CTTAAG C^TTAAG
AflIII 2018 ACACGT A^C[GA][CT]GT
Alw44I 1747 GTGCAC G^TGCAC
AlwNI 865 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1747 GTGCAC G^TGCAC
BamHI 1206 GGATCC G^GATCC
BbrPI 2019 CACGTG CAC^GTG
BbvCI 954 GCTGAGG CCTCAGC(-5/-2)
BfrI 1546 CTTAAG C^TTAAG
BlpI 1594 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1594 GCTAAGC GC^T[ACGT]AGC
BsaAI 2019 CACGTG [CT]AC^GT[GA]
BsaI 1098 GAGACC GGTCTC(1/5)
BsaWI 1948 TCCGGT [AT]^CCGG[AT]
BsgI 347 GTGCAG GTGCAG(16/14)
BsmBI 634 GAGACG CGTCTC(1/5)
BsrDI 846 CATTGC GCAATG(2/0)
CelII 1594 GCTAAGC GC^T[ACGT]AGC
EcoNI 834 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeII 2020 ACGT A^CGT
MseI 1547 TTAA T^TAA
PmaCI 2019 CACGTG CAC^GTG
PmlI 2019 CACGTG CAC^GTG
SexAI 1685 ACCTGGT A^CC[AT]GGT
SmlI 1546 CTTAAG C^T[CT][GA]AG
Tru9I 1547 TTAA T^TAA
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# TWO CUT ENZYMES ( 31 enzymes) #
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AfaI 588 GTAC GT^AC
1074 GTAC
AocI 778 CCTGAGG CC^T[ACGT]AGG
1240 CCTGAGG
Aor51HI 351 AGCGCT AGC^GCT
1130 AGCGCT
ApaI 284 GGGCCC GGGCC^C
399 GGGCCC
AviII 382 TGCGCA TGC^GCA
1554 TGCGCA
BfaI 715 CTAG C^TAG
2144 CTAG
BsmAI 634 GAGAC GTCTC(1/5)
1098 GAGAC
BspMI 476 ACCTGC ACCTGC(4/8)
560 ACCTGC
BsrFI 162 GCCGGC [GA]^CCGG[CT]
878 GCCGGC
BssHII 135 GCGCGC G^CGCGC
434 GCGCGC
BstYI 510 GGATCT [GA]^GATC[CT]
1206 GGATCC
Bsu36I 778 CCTGAGG CC^T[ACGT]AGG
1240 CCTGAGG
Cfr10I 162 GCCGGC [GA]^CCGG[CT]
878 GCCGGC
DraIII 1684 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1741 CACCCAGTG
DrdI 75 GACCCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1756 GACAACTGCGTC
Eco47III 351 AGCGCT AGC^GCT
1130 AGCGCT
Eco81I 778 CCTGAGG CC^T[ACGT]AGG
1240 CCTGAGG
FspI 382 TGCGCA TGC^GCA
1554 TGCGCA
MaeI 715 CTAG C^TAG
2144 CTAG
MflI 510 GGATCT [GA]^GATC[CT]
1206 GGATCC
NaeI 162 GCCGGC GCC^GGC
878 GCCGGC
NcoI 1178 CCATGG C^CATGG
1965 CCATGG
NgoMIV 162 GCCGGC G^CCGGC
878 GCCGGC
PshAI 593 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1732 GACCTGAGTC
RsaI 588 GTAC GT^AC
1074 GTAC
SapI 724 GCTCTTC GCTCTTC(1/4)
1029 GCTCTTC
StuI 1282 AGGCCT AGG^CCT
1640 AGGCCT
TaqI 1529 TCGA T^CGA
1955 TCGA
TthHB8I 1529 TCGA T^CGA
1955 TCGA
XcmI 1178 CCATGGAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2166 CCAAGACAAGACTGG
XhoII 510 GGATCT [GA]^GATC[CT]
1206 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TfiI
TseI Tsp45I TspRI XmaCI XmaI