Restriction map for
pFN21AE3951
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AvrII BbrPI
BcgI BciVI BfaI BfrI BglII
BlnI BpmI BsiEI BsmI Bsp1407I
BspDI BspHI BspMI BsrDI BsrGI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT22I HpaI KpnI MaeI MfeI
MluI MunI NdeI NheI NotI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tsp509I XbaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 999 TCCGGA T^CCGGA
AscI 191 GGCGCGCC GG^CGCGCC
AspI 1536 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1995 GGATCC G^GATCC
BclI 272 TGATCA T^GATCA
BglI 1190 GCCTCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 532 GAGACC GGTCTC(1/5)
BsaWI 999 TCCGGA [AT]^CCGG[AT]
BseAI 999 TCCGGA T^CCGGA
BsgI 466 CTGCAC GTGCAG(16/14)
BsiWI 434 CGTACG C^GTACG
BspEI 999 TCCGGA T^CCGGA
BspLU11I 1523 ACATGT A^CATGT
BssHII 192 GCGCGC G^CGCGC
BssSI 2020 CACGAG CACGAG(-5/-1)
BstXI 866 CCAGGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 495 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2262 GATATC GAT^ATC
FbaI 272 TGATCA T^GATCA
FseI 342 GGCCGGCC GGCCGG^CC
HincII 1615 GTCAAC GT[CT]^[GA]AC
HindII 1615 GTCAAC GT[CT]^[GA]AC
HindIII 2223 AAGCTT A^AGCTT
MroI 999 TCCGGA T^CCGGA
MseI 1130 TTAA T^TAA
NruI 576 TCGCGA TCG^CGA
PaeR7I 1180 CTCGAG C^TCGAG
PflFI 1536 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1532 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 842 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 2006 CAGCTG CAG^CTG
SapI 834 GCTCTTC GCTCTTC(1/4)
SfcI 2100 CTATAG C^T[GA][CT]AG
SfiI 1189 GGCCTCTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 1130 TTAA T^TAA
Tth111I 1536 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1532 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1180 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflIII 482 ACGTGT A^C[GA][CT]GT
1523 ACATGT
AlwNI 1657 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2003 CAGCAGCTG
BbsI 2062 GAAGAC GAAGAC(2/6)
427 GTCTTC
BbvCI 1200 CCTCAGC CCTCAGC(-5/-2)
583 GCTGAGG
BlpI 3 GCTGAGC GC^T[ACGT]AGC
1405 GCTGAGC
Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC
1405 GCTGAGC
BpuAI 2062 GAAGAC GAAGAC(2/6)
427 GTCTTC
BsaAI 436 TACGTG [CT]AC^GT[GA]
1048 TACGTG
BsaBI 267 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1959 GATTGAGATC
BsrI 2173 ACTGG ACTGG(1/-1)
2201 ACTGG
BstAPI 1656 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2002 GCAGCAGCTGC
BstEII 966 GGTGACC G^GT[ACGT]ACC
1494 GGTCACC
BstPI 966 GGTGACC G^GT[ACGT]ACC
1494 GGTCACC
BstYI 1977 AGATCC [GA]^GATC[CT]
1995 GGATCC
CelII 3 GCTGAGC GC^T[ACGT]AGC
1405 GCTGAGC
DraIII 798 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
1288 CACTTCGTG
EarI 835 CTCTTC CTCTTC(1/4)
889 CTCTTC
EcoO65I 966 GGTGACC G^GT[ACGT]ACC
1494 GGTCACC
Ksp632I 835 CTCTTC CTCTTC(1/4)
889 CTCTTC
MamI 267 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1959 GATTGAGATC
MflI 1977 AGATCC [GA]^GATC[CT]
1995 GGATCC
NspI 1523 ACATGT [GA]CATG^[CT]
1628 GCATGT
SmlI 724 CTCAAG C^T[CT][GA]AG
1180 CTCGAG
XhoII 1977 AGATCC [GA]^GATC[CT]
1995 GGATCC
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
AviII BalI BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfoI SmaI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XmaCI XmaI