Restriction map for
pFN21AE3347
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaLI
AscI Asp718I AspEI AspHI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BseRI BsiHKAI BsiWI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraII DraIII EaeI EagI
Eam1105I EclXI Eco52I EcoO109I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HpaI KasI KpnI KspI MluI
MluNI MroI MscI NaeI NarI
NciI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacI SacII SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 485 GTCGAC GT^[AC][GT]AC
AccII 147 CGCG CG^CG
AflIII 466 ACATGT A^C[GA][CT]GT
AocI 2909 CCTCAGG CC^T[ACGT]AGG
ApaI 299 GGGCCC GGGCC^C
AseI 2773 ATTAAT AT^TAAT
AsnI 2773 ATTAAT AT^TAAT
Asp700I 2353 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 34 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 190 GGACC G^G[AT]CC
BbsI 1845 GTCTTC GAAGAC(2/6)
BpuAI 1845 GTCTTC GAAGAC(2/6)
BsiEI 12 CGACCG CG[GA][CT]^CG
BsmBI 2420 CGTCTC CGTCTC(1/5)
Bsp1407I 1194 TGTACA T^GTACA
BspHI 681 TCATGA T^CATGA
BspLU11I 466 ACATGT A^CATGT
BsrFI 14 ACCGGC [GA]^CCGG[CT]
BsrGI 1194 TGTACA T^GTACA
BstEII 2758 GGTAACC G^GT[ACGT]ACC
BstPI 2758 GGTAACC G^GT[ACGT]ACC
BstUI 147 CGCG CG^CG
Bsu36I 2909 CCTCAGG CC^T[ACGT]AGG
Cfr10I 14 ACCGGC [GA]^CCGG[CT]
DrdI 927 GACTAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2909 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2758 GGTAACC G^GT[ACGT]ACC
EcoT14I 3056 CCATGG C^C[AT][AT]GG
HgaI 143 GCGTC GACGC(5/10)
HincII 485 GTCGAC GT[CT]^[GA]AC
HindII 485 GTCGAC GT[CT]^[GA]AC
MaeII 1666 ACGT A^CGT
MfeI 365 CAATTG C^AATTG
MunI 365 CAATTG C^AATTG
MvnI 147 CGCG CG^CG
NcoI 3056 CCATGG C^CATGG
NheI 1033 GCTAGC G^CTAGC
PflFI 34 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2811 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2773 ATTAAT AT^TAAT
RcaI 681 TCATGA T^CATGA
SalI 485 GTCGAC G^TCGAC
ScaI 1810 AGTACT AGT^ACT
SmlI 1046 CTCAAG C^T[CT][GA]AG
SspBI 1194 TGTACA T^GTACA
StyI 3056 CCATGG C^C[AT][AT]GG
TaqI 486 TCGA T^CGA
Tth111I 34 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 486 TCGA T^CGA
XmnI 2353 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwNI 446 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1901 CAGCCCCTG
Aor51HI 839 AGCGCT AGC^GCT
1872 AGCGCT
AvaI 1800 CTCGGG C^[CT]CG[GA]G
2090 CCCGAG
BclI 636 TGATCA T^GATCA
2142 TGATCA
BglII 1140 AGATCT A^GATCT
2043 AGATCT
BsaBI 673 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2038 GATCAAGATC
BsaI 3 GGTCTC GGTCTC(1/5)
39 GGTCTC
BsmFI 254 GTCCC GGGAC(10/14)
2826 GTCCC
BsoBI 1800 CTCGGG C^[CT]CG[GA]G
2090 CCCGAG
BspMI 756 ACCTGC ACCTGC(4/8)
2652 GCAGGT
BsrDI 1562 CATTGC GCAATG(2/0)
1760 GCAATG
BstXI 258 CCATAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
564 CCAATACTGTGG
DraI 273 TTTAAA TTT^AAA
2793 TTTAAA
DsaI 193 CCGTGG C^C[GA][CT]GG
3056 CCATGG
EarI 2525 CTCTTC CTCTTC(1/4)
431 GAAGAG
Eco47III 839 AGCGCT AGC^GCT
1872 AGCGCT
EcoNI 1336 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2491 CCTCATATAGG
FauI 111 CCCGC CCCGC(4/6)
1353 GCGGG
FbaI 636 TGATCA T^GATCA
2142 TGATCA
HaeII 839 AGCGCT [GA]GCGC^[CT]
1872 AGCGCT
HindIII 158 AAGCTT A^AGCTT
1293 AAGCTT
Ksp632I 2525 CTCTTC CTCTTC(1/4)
431 GAAGAG
MamI 673 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2038 GATCAAGATC
MslI 259 CATAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1969 CATATGTATG
NdeI 632 CATATG CA^TATG
1969 CATATG
NspI 466 ACATGT [GA]CATG^[CT]
505 ACATGC
SfaNI 153 GCATC GCATC(5/9)
1513 GCATC
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanI BanII BbvCI BbvI
BfaI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII