Restriction map for
pFN21AE6734
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
AviII AvrII BamHI BbeI BcgI
BciVI BclI BfrI BlnI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoRV EcoT22I
FbaI FseI FspI HpaI KasI
MamI MroI MseI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I XbaI XcmI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 785 GGTACC G^GTACC
AcyI 1000 GACGCC G[GA]^CG[CT]C
AhdI 2261 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1359 AGCGCT AGC^GCT
Asp700I 1896 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 785 GGTACC G^GTACC
AspEI 2261 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1975 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 93 CACGTG CAC^GTG
BbvCI 516 CCTCAGC CCTCAGC(-5/-2)
BfaI 37 CTAG C^TAG
BglII 1529 AGATCT A^GATCT
BlpI 948 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 948 GCTCAGC GC^T[ACGT]AGC
BsaAI 93 CACGTG [CT]AC^GT[GA]
BsaHI 1000 GACGCC G[GA]^CG[CT]C
BspLU11I 1502 ACATGT A^CATGT
BsrI 1460 ACTGG ACTGG(1/-1)
BssSI 1324 CACGAG CACGAG(-5/-1)
CelII 948 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2261 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1359 AGCGCT AGC^GCT
EcoRI 1132 GAATTC G^AATTC
Hin1I 1000 GACGCC G[GA]^CG[CT]C
HincII 1981 GTCGAC GT[CT]^[GA]AC
HindII 1981 GTCGAC GT[CT]^[GA]AC
HindIII 2094 AAGCTT A^AGCTT
KpnI 785 GGTACC GGTAC^C
MaeI 37 CTAG C^TAG
MfeI 290 CAATTG C^AATTG
MluI 1289 ACGCGT A^CGCGT
MslI 880 CACCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 290 CAATTG C^AATTG
NspI 1502 ACATGT [GA]CATG^[CT]
PaeR7I 1064 CTCGAG C^TCGAG
PflFI 1975 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 93 CACGTG CAC^GTG
PmlI 93 CACGTG CAC^GTG
SalI 1981 GTCGAC G^TCGAC
Tth111I 1975 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1064 CTCGAG C^TCGAG
XmnI 1896 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 76 GTCTAC GT^[AC][GT]AC
1981 GTCGAC
AcsI 172 AAATTT [GA]^AATT[CT]
1132 GAATTC
AflIII 1289 ACGCGT A^C[GA][CT]GT
1502 ACATGT
AlwI 2209 GATCC GGATC(4/5)
212 GGATC
ApoI 172 AAATTT [GA]^AATT[CT]
1132 GAATTC
AspHI 403 GAGCAC G[AT]GC[AT]^C
2387 GTGCTC
BalI 1794 TGGCCA TGG^CCA
1808 TGGCCA
BbsI 1278 GAAGAC GAAGAC(2/6)
1972 GAAGAC
BglI 3 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
614 GCCTCGAAGGC
BpuAI 1278 GAAGAC GAAGAC(2/6)
1972 GAAGAC
BsgI 43 GTGCAG GTGCAG(16/14)
634 GTGCAG
BsiHKAI 403 GAGCAC G[AT]GC[AT]^C
2387 GTGCTC
BsrFI 125 ACCGGC [GA]^CCGG[CT]
2485 ACCGGC
BstEII 430 GGTGACC G^GT[ACGT]ACC
1329 GGTGACC
BstPI 430 GGTGACC G^GT[ACGT]ACC
1329 GGTGACC
BstYI 1529 AGATCT [GA]^GATC[CT]
2208 AGATCC
Cfr10I 125 ACCGGC [GA]^CCGG[CT]
2485 ACCGGC
DraIII 1324 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2342 CACAGTGTG
DrdI 265 GACGTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
448 GACCCCATGGTC
EcoNI 1482 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1863 CCTGCCAGAGG
EcoO65I 430 GGTGACC G^GT[ACGT]ACC
1329 GGTGACC
HaeII 128 GGCGCT [GA]GCGC^[CT]
1359 AGCGCT
KspI 7 CCGCGG CCGC^GG
1570 CCGCGG
MflI 1529 AGATCT [GA]^GATC[CT]
2208 AGATCC
MluNI 1794 TGGCCA TGG^CCA
1808 TGGCCA
MscI 1794 TGGCCA TGG^CCA
1808 TGGCCA
PflMI 859 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2073 CCAAGACGTGG
SacII 7 CCGCGG CCGC^GG
1570 CCGCGG
SmaI 882 CCCGGG CCC^GGG
1715 CCCGGG
SmlI 1064 CTCGAG C^T[CT][GA]AG
1203 CTTGAG
Van91I 859 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2073 CCAAGACGTGG
XhoII 1529 AGATCT [GA]^GATC[CT]
2208 AGATCC
XmaCI 882 CCCGGG C^CCGGG
1715 CCCGGG
XmaI 882 CCCGGG C^CCGGG
1715 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AocI ApaI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BssKI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I