Restriction map for
pFN21AE4518
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AclI AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BglI BglII BlnI BpuAI
BsaI BseRI BsiEI BsiWI BspDI
BsrDI BsrFI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspI SwaI Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 211 GACGTC GACGT^C
AccI 199 GTCTAC GT^[AC][GT]AC
AccIII 1391 TCCGGA T^CCGGA
AcsI 980 GAATTC [GA]^AATT[CT]
AflII 121 CTTAAG C^TTAAG
AhdI 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 993 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 268 GGGCCC GGGCC^C
ApoI 980 GAATTC [GA]^AATT[CT]
AspEI 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 628 GAGCTC G[AT]GC[AT]^C
BamHI 689 GGATCC G^GATCC
BanI 93 GGTGCC G^G[CT][GA]CC
BclI 758 TGATCA T^GATCA
BfrI 121 CTTAAG C^TTAAG
BsaAI 274 TACGTG [CT]AC^GT[GA]
BsaBI 174 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1391 TCCGGA [AT]^CCGG[AT]
BseAI 1391 TCCGGA T^CCGGA
BsgI 1081 CTGCAC GTGCAG(16/14)
BsiHKAI 628 GAGCTC G[AT]GC[AT]^C
BsmBI 1525 CGTCTC CGTCTC(1/5)
BspEI 1391 TCCGGA T^CCGGA
BspLU11I 161 ACATGT A^CATGT
BssHII 19 GCGCGC G^CGCGC
BssSI 697 CTCGTG CACGAG(-5/-1)
BstXI 410 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 694 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 980 GAATTC G^AATTC
FbaI 758 TGATCA T^GATCA
HincII 727 GTTGAC GT[CT]^[GA]AC
HindII 727 GTTGAC GT[CT]^[GA]AC
MamI 174 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1391 TCCGGA T^CCGGA
MseI 122 TTAA T^TAA
NcoI 1262 CCATGG C^CATGG
PaeR7I 340 CTCGAG C^TCGAG
PflMI 693 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 628 GAGCTC GAGCT^C
SapI 651 GCTCTTC GCTCTTC(1/4)
SbfI 998 CCTGCAGG CCTGCA^GG
ScaI 578 AGTACT AGT^ACT
SmaI 1424 CCCGGG CCC^GGG
SphI 1585 GCATGC GCATG^C
Sse8387I 998 CCTGCAGG CCTGCA^GG
Tru9I 122 TTAA T^TAA
Tsp509I 981 AATT ^AATT
Van91I 693 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 340 CTCGAG C^TCGAG
XmaCI 1424 CCCGGG C^CCGGG
XmaI 1424 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 43 GGCGTC G[GA]^CG[CT]C
211 GACGTC
AflIII 161 ACATGT A^C[GA][CT]GT
275 ACGTGT
AvaI 340 CTCGAG C^[CT]CG[GA]G
1424 CCCGGG
AviII 131 TGCGCA TGC^GCA
1562 TGCGCA
BalI 1410 TGGCCA TGG^CCA
1483 TGGCCA
BsaHI 43 GGCGTC G[GA]^CG[CT]C
211 GACGTC
BsmAI 1273 GAGAC GTCTC(1/5)
1526 GTCTC
BsmI 484 GCATTC GAATGC(1/-1)
738 GCATTC
BsoBI 340 CTCGAG C^[CT]CG[GA]G
1424 CCCGGG
Bsp1407I 278 TGTACA T^GTACA
857 TGTACA
BsrBI 1254 GAGCGG CCGCTC(-3/-3)
1542 GAGCGG
BsrGI 278 TGTACA T^GTACA
857 TGTACA
DrdI 394 GACTACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
798 GACTCCTGAGTC
DsaI 378 CCACGG C^C[GA][CT]GG
1262 CCATGG
EarI 652 CTCTTC CTCTTC(1/4)
1277 CTCTTC
FspI 131 TGCGCA TGC^GCA
1562 TGCGCA
Hin1I 43 GGCGTC G[GA]^CG[CT]C
211 GACGTC
Ksp632I 652 CTCTTC CTCTTC(1/4)
1277 CTCTTC
MluNI 1410 TGGCCA TGG^CCA
1483 TGGCCA
MscI 1410 TGGCCA TGG^CCA
1483 TGGCCA
MslI 81 CATGATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
399 CATCGTCATG
PstI 999 CTGCAG CTGCA^G
1162 CTGCAG
PvuII 1165 CAGCTG CAG^CTG
1297 CAGCTG
SmlI 121 CTTAAG C^T[CT][GA]AG
340 CTCGAG
SspBI 278 TGTACA T^GTACA
857 TGTACA
StuI 833 AGGCCT AGG^CCT
1620 AGGCCT
TaqI 137 TCGA T^CGA
341 TCGA
TfiI 564 GAATC G^A[AT]TC
1136 GAATC
TthHB8I 137 TCGA T^CGA
341 TCGA
XcmI 1398 CCAAGTAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1486 CCAACCGCAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaJI BsiYI
BslI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI XhoII