Restriction map for
pFN21AB6293
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# NO CUT ENZYMES ( 92 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AvrII BbeI BcgI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BseAI
BsgI BsiWI BsmI BspDI BspEI
BspHI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 1458 GAATTT [GA]^AATT[CT]
ApoI 1458 GAATTT [GA]^AATT[CT]
Asp700I 1458 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 862 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1646 TGCGCA TGC^GCA
BalI 1883 TGGCCA TGG^CCA
BanI 1318 GGCACC G^G[CT][GA]CC
BbsI 868 GTCTTC GAAGAC(2/6)
BciVI 1857 GTATCC GTATCC(6/5)
BclI 1530 TGATCA T^GATCA
BfaI 1417 CTAG C^TAG
BpuAI 868 GTCTTC GAAGAC(2/6)
BsaI 781 GAGACC GGTCTC(1/5)
BsaWI 1826 ACCGGA [AT]^CCGG[AT]
Bsp1407I 440 TGTACA T^GTACA
BspLU11I 413 ACATGT A^CATGT
BsrGI 440 TGTACA T^GTACA
DrdI 217 GACTACGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1530 TGATCA T^GATCA
FspI 1646 TGCGCA TGC^GCA
HincII 907 GTTGAC GT[CT]^[GA]AC
HindII 907 GTTGAC GT[CT]^[GA]AC
KspI 1514 CCGCGG CCGC^GG
MaeI 1417 CTAG C^TAG
MluNI 1883 TGGCCA TGG^CCA
MscI 1883 TGGCCA TGG^CCA
NotI 376 GCGGCCGC GC^GGCCGC
NspI 413 ACATGT [GA]CATG^[CT]
PflFI 862 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 2310 CAGCTG CAG^CTG
SacI 304 GAGCTC GAGCT^C
SacII 1514 CCGCGG CCGC^GG
SmlI 2140 CTCAAG C^T[CT][GA]AG
SspBI 440 TGTACA T^GTACA
StuI 159 AGGCCT AGG^CCT
TfiI 829 GATTC G^A[AT]TC
Tth111I 862 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1458 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AatII 580 GACGTC GACGT^C
646 GACGTC
AflIII 413 ACATGT A^C[GA][CT]GT
2264 ACGTGT
AhdI 88 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1615 GACTGGCGGTC
AspEI 88 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1615 GACTGGCGGTC
AspHI 271 GTGCTC G[AT]GC[AT]^C
304 GAGCTC
BamHI 44 GGATCC G^GATCC
1307 GGATCC
BglII 764 AGATCT A^GATCT
1491 AGATCT
BsiHKAI 271 GTGCTC G[AT]GC[AT]^C
304 GAGCTC
BspMI 501 GCAGGT ACCTGC(4/8)
2355 GCAGGT
BsrDI 1649 GCAATG GCAATG(2/0)
2408 GCAATG
BssHII 655 GCGCGC G^CGCGC
657 GCGCGC
BssSI 268 CTCGTG CACGAG(-5/-1)
1759 CTCGTG
BstAPI 944 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2270 GCAAGGACTGC
BstXI 1139 CCAGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1371 CCACGTGGCTGG
Eam1105I 88 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1615 GACTGGCGGTC
MaeIII 514 GTGAC ^GT[ACGT]AC
2136 GTCAC
SapI 927 GAAGAGC GCTCTTC(1/4)
2391 GCTCTTC
SmaI 533 CCCGGG CCC^GGG
1302 CCCGGG
Tsp45I 514 GTGAC ^GT[GC]AC
2136 GTCAC
Tsp509I 431 AATT ^AATT
1459 AATT
XmaCI 533 CCCGGG C^CCGGG
1302 CCCGGG
XmaI 533 CCCGGG C^CCGGG
1302 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanII
BbrPI BbvCI BbvI BcnI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PmaCI PmlI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TspRI TthHB8I Van91I XcmI
XhoII