Restriction map for
pFN21AE1172
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvaI AvrII BamHI
BanI BbeI BbrPI BbvCI BbvI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT14I
FauI Fnu4HI FseI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII ItaI KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TseI Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AciI 439 CCGC CCGC(-3/-1)
Asp700I 836 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 81 TGCGCA TGC^GCA
BalI 38 TGGCCA TGG^CCA
BanII 276 GAGCTC G[GA]GC[CT]^C
BclI 501 TGATCA T^GATCA
BseRI 273 GAGGAG GAGGAG(10/8)
BsgI 1827 GTGCAG GTGCAG(16/14)
BsiYI 6 CCTTACTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 6 CCTTACTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 698 GAATGC GAATGC(1/-1)
BstBI 833 TTCGAA TT^CGAA
DraI 477 TTTAAA TTT^AAA
DraII 15 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1821 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 6 CCTTACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 15 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 700 ATGCAT ATGCA^T
FbaI 501 TGATCA T^GATCA
FspI 81 TGCGCA TGC^GCA
MaeII 104 ACGT A^CGT
MluNI 38 TGGCCA TGG^CCA
MscI 38 TGGCCA TGG^CCA
NsiI 700 ATGCAT ATGCA^T
NspV 833 TTCGAA TT^CGAA
PleI 201 GACTC GAGTC(4/5)
PpuMI 15 GGGACCC [GA]G^G[AT]CC[CT]
PstI 205 CTGCAG CTGCA^G
SacI 276 GAGCTC GAGCT^C
SapI 744 GCTCTTC GCTCTTC(1/4)
SfuI 833 TTCGAA TT^CGAA
XmnI 836 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 937 GTAC GT^AC
1425 GTAC
AlwI 193 GATCC GGATC(4/5)
232 GATCC
AspHI 276 GAGCTC G[AT]GC[AT]^C
568 GAGCAC
AvaII 16 GGACC G^G[AT]CC
75 GGACC
BfaI 1018 CTAG C^TAG
1211 CTAG
BglII 1247 AGATCT A^GATCT
1751 AGATCT
BmyI 276 GAGCTC G[AGT]GC[ACT]^C
568 GAGCAC
BsaI 1218 GAGACC GGTCTC(1/5)
132 GGTCTC
BsiHKAI 276 GAGCTC G[AT]GC[AT]^C
568 GAGCAC
BsmAI 1218 GAGAC GTCTC(1/5)
133 GTCTC
BsmFI 15 GGGAC GGGAC(10/14)
101 GGGAC
Bsp1286I 276 GAGCTC G[AGT]GC[ACT]^C
568 GAGCAC
BstAPI 242 GCAGGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1820 GCACCCTGTGC
BstF5I 33 GGATG GGATG(2/0)
1228 GGATG
CfoI 82 GCGC GCG^C
1173 GCGC
EaeI 38 TGGCCA [CT]^GGCC[GA]
573 CGGCCA
FokI 33 GGATG GGATG(9/13)
1228 GGATG
HhaI 82 GCGC GCG^C
1173 GCGC
HinP1I 82 GCGC G^CGC
1173 GCGC
MaeI 1018 CTAG C^TAG
1211 CTAG
RsaI 937 GTAC GT^AC
1425 GTAC
SexAI 128 ACCTGGT A^CC[AT]GGT
1520 ACCTGGT
SfcI 205 CTGCAG C^T[GA][CT]AG
358 CTACAG
StuI 1639 AGGCCT AGG^CCT
1686 AGGCCT
TfiI 143 GAATC G^A[AT]TC
329 GATTC
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AluI ApoI AspI BbsI
BpmI BpuAI BsaJI BspHI BsrI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRI EcoRII
HaeIII HindIII HinfI HphI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PflFI RcaI Sau3AI
Sau96I ScrFI SfaNI TaqI Tru9I
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII