################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AvrII BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT14I FauI Fnu4HI FseI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII ItaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TseI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AciI 439 CCGC CCGC(-3/-1) Asp700I 836 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 81 TGCGCA TGC^GCA BalI 38 TGGCCA TGG^CCA BanII 276 GAGCTC G[GA]GC[CT]^C BclI 501 TGATCA T^GATCA BseRI 273 GAGGAG GAGGAG(10/8) BsgI 1827 GTGCAG GTGCAG(16/14) BsiYI 6 CCTTACTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 6 CCTTACTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 698 GAATGC GAATGC(1/-1) BstBI 833 TTCGAA TT^CGAA DraI 477 TTTAAA TTT^AAA DraII 15 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 1821 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 6 CCTTACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 15 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT22I 700 ATGCAT ATGCA^T FbaI 501 TGATCA T^GATCA FspI 81 TGCGCA TGC^GCA MaeII 104 ACGT A^CGT MluNI 38 TGGCCA TGG^CCA MscI 38 TGGCCA TGG^CCA NsiI 700 ATGCAT ATGCA^T NspV 833 TTCGAA TT^CGAA PleI 201 GACTC GAGTC(4/5) PpuMI 15 GGGACCC [GA]G^G[AT]CC[CT] PstI 205 CTGCAG CTGCA^G SacI 276 GAGCTC GAGCT^C SapI 744 GCTCTTC GCTCTTC(1/4) SfuI 833 TTCGAA TT^CGAA XmnI 836 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 937 GTAC GT^AC 1425 GTAC AlwI 193 GATCC GGATC(4/5) 232 GATCC AspHI 276 GAGCTC G[AT]GC[AT]^C 568 GAGCAC AvaII 16 GGACC G^G[AT]CC 75 GGACC BfaI 1018 CTAG C^TAG 1211 CTAG BglII 1247 AGATCT A^GATCT 1751 AGATCT BmyI 276 GAGCTC G[AGT]GC[ACT]^C 568 GAGCAC BsaI 1218 GAGACC GGTCTC(1/5) 132 GGTCTC BsiHKAI 276 GAGCTC G[AT]GC[AT]^C 568 GAGCAC BsmAI 1218 GAGAC GTCTC(1/5) 133 GTCTC BsmFI 15 GGGAC GGGAC(10/14) 101 GGGAC Bsp1286I 276 GAGCTC G[AGT]GC[ACT]^C 568 GAGCAC BstAPI 242 GCAGGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1820 GCACCCTGTGC BstF5I 33 GGATG GGATG(2/0) 1228 GGATG CfoI 82 GCGC GCG^C 1173 GCGC EaeI 38 TGGCCA [CT]^GGCC[GA] 573 CGGCCA FokI 33 GGATG GGATG(9/13) 1228 GGATG HhaI 82 GCGC GCG^C 1173 GCGC HinP1I 82 GCGC G^CGC 1173 GCGC MaeI 1018 CTAG C^TAG 1211 CTAG RsaI 937 GTAC GT^AC 1425 GTAC SexAI 128 ACCTGGT A^CC[AT]GGT 1520 ACCTGGT SfcI 205 CTGCAG C^T[GA][CT]AG 358 CTACAG StuI 1639 AGGCCT AGG^CCT 1686 AGGCCT TfiI 143 GAATC G^A[AT]TC 329 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AcsI AluI ApoI AspI BbsI BpmI BpuAI BsaJI BspHI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRI EcoRII HaeIII HindIII HinfI HphI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PflFI RcaI Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII