Restriction map for
pFN21AE9663
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AclI AcsI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaII
AviII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BpuAI BsaAI BsaBI BsaI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI MamI MfeI MluI
MunI NarI NdeI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 36 GACGTC GACGT^C
AccI 1017 GTCTAC GT^[AC][GT]AC
AccII 10 CGCG CG^CG
AccIII 743 TCCGGA T^CCGGA
AlwNI 583 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 859 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 331 CCCGAG C^[CT]CG[GA]G
AvrII 684 CCTAGG C^CTAGG
BamHI 355 GGATCC G^GATCC
BanI 132 GGCACC G^G[CT][GA]CC
BglI 397 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 684 CCTAGG C^CTAGG
BlpI 979 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 979 GCTCAGC GC^T[ACGT]AGC
BsaWI 743 TCCGGA [AT]^CCGG[AT]
BseAI 743 TCCGGA T^CCGGA
BsmFI 219 GGGAC GGGAC(10/14)
BsoBI 331 CCCGAG C^[CT]CG[GA]G
Bsp1407I 140 TGTACA T^GTACA
BspEI 743 TCCGGA T^CCGGA
BspMI 534 ACCTGC ACCTGC(4/8)
BsrFI 622 GCCGGC [GA]^CCGG[CT]
BsrGI 140 TGTACA T^GTACA
BstAPI 237 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 10 CGCG CG^CG
BstYI 355 GGATCC [GA]^GATC[CT]
CelII 979 GCTCAGC GC^T[ACGT]AGC
Cfr10I 622 GCCGGC [GA]^CCGG[CT]
DdeI 980 CTCAG C^T[ACGT]AG
DrdI 36 GACGTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 351 GCGGG CCCGC(4/6)
HaeII 508 AGCGCC [GA]GCGC^[CT]
HgaI 1108 GACGC GACGC(5/10)
KspI 9 CCGCGG CCGC^GG
MaeII 37 ACGT A^CGT
MflI 355 GGATCC [GA]^GATC[CT]
MroI 743 TCCGGA T^CCGGA
MseI 674 TTAA T^TAA
MslI 1080 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 10 CGCG CG^CG
NaeI 622 GCCGGC GCC^GGC
NcoI 795 CCATGG C^CATGG
NgoMIV 622 GCCGGC G^CCGGC
NheI 339 GCTAGC G^CTAGC
SacII 9 CCGCGG CCGC^GG
SapI 265 GAAGAGC GCTCTTC(1/4)
SspBI 140 TGTACA T^GTACA
TaqI 4 TCGA T^CGA
Tru9I 674 TTAA T^TAA
Tsp509I 616 AATT ^AATT
TthHB8I 4 TCGA T^CGA
XcmI 359 CCAAAGGAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 355 GGATCC [GA]^GATC[CT]
XmnI 859 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 36 GACGTC G[GA]^CG[CT]C
1108 GACGCC
ApaI 461 GGGCCC GGGCC^C
757 GGGCCC
BanII 461 GGGCCC G[GA]GC[CT]^C
757 GGGCCC
BcnI 233 CCCGG CC^[GC]GG
464 CCCGG
BfaI 340 CTAG C^TAG
685 CTAG
BsaHI 36 GACGTC G[GA]^CG[CT]C
1108 GACGCC
BsgI 845 GTGCAG GTGCAG(16/14)
1087 GTGCAG
BsmAI 206 GAGAC GTCTC(1/5)
411 GTCTC
BsrDI 924 CATTGC GCAATG(2/0)
194 GCAATG
CfoI 509 GCGC GCG^C
788 GCGC
DpnII 356 GATC ^GATC
819 GATC
DraII 460 GGGGCCC [GA]G^G[ACGT]CC[CT]
757 GGGCCCT
EcoO109I 460 GGGGCCC [GA]G^G[ACGT]CC[CT]
757 GGGCCCT
HhaI 509 GCGC GCG^C
788 GCGC
Hin1I 36 GACGTC G[GA]^CG[CT]C
1108 GACGCC
HinP1I 509 GCGC G^CGC
788 GCGC
HphI 487 GGTGA GGTGA(8/7)
293 TCACC
MaeI 340 CTAG C^TAG
685 CTAG
MboI 356 GATC ^GATC
819 GATC
NciI 233 CCCGG CC^[GC]GG
464 CCCGG
NdeII 356 GATC ^GATC
819 GATC
NlaIII 144 CATG CATG^
796 CATG
PleI 59 GAGTC GAGTC(4/5)
290 GAGTC
PvuII 277 CAGCTG CAG^CTG
982 CAGCTG
Sau3AI 356 GATC ^GATC
819 GATC
SfaNI 81 GATGC GCATC(5/9)
1079 GCATC
Tsp45I 226 GTCAC ^GT[GC]AC
292 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BbvI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DsaI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeIII MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NlaIV RsaI Sau96I ScrFI StyI
TfiI TseI TspRI