Restriction map for
pFN21AE4592
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII ApoI AscI AseI AsnI
Asp718I AspI BbrPI BcgI BclI
BfrI BsaBI BseAI BsiEI BsmBI
BspDI BspEI BspHI BstBI ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRI FbaI FseI HgaI
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SfuI SnaBI SpeI SspI SwaI
TfiI Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 1278 GTATAC GT^[AC][GT]AC
AcyI 1791 GGCGCC G[GA]^CG[CT]C
AflIII 3044 ACATGT A^C[GA][CT]GT
AgeI 1809 ACCGGT A^CCGGT
AhdI 22 GACTCGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3229 GTGCAC G^TGCAC
ApaLI 3229 GTGCAC G^TGCAC
Asp700I 3337 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 22 GACTCGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2651 TGCGCA TGC^GCA
AvrII 361 CCTAGG C^CTAGG
BalI 2378 TGGCCA TGG^CCA
BbeI 1791 GGCGCC GGCGC^C
BbsI 3 GAAGAC GAAGAC(2/6)
BbvCI 2194 GCTGAGG CCTCAGC(-5/-2)
BciVI 1969 GGATAC GTATCC(6/5)
BglII 224 AGATCT A^GATCT
BlnI 361 CCTAGG C^CTAGG
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaAI 2716 TACGTG [CT]AC^GT[GA]
BsaHI 1791 GGCGCC G[GA]^CG[CT]C
BsiWI 1916 CGTACG C^GTACG
BsmI 2551 GAATGC GAATGC(1/-1)
Bsp1407I 2771 TGTACA T^GTACA
BspLU11I 3044 ACATGT A^CATGT
BsrGI 2771 TGTACA T^GTACA
Bst1107I 1278 GTATAC GTA^TAC
BstEII 1821 GGTCACC G^GT[ACGT]ACC
BstPI 1821 GGTCACC G^GT[ACGT]ACC
BstXI 830 CCACTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1278 GTATAC GTA^TAC
DraIII 3505 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 22 GACTCGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1821 GGTCACC G^GT[ACGT]ACC
EcoT22I 935 ATGCAT ATGCA^T
FspI 2651 TGCGCA TGC^GCA
Hin1I 1791 GGCGCC G[GA]^CG[CT]C
HincII 199 GTCAAC GT[CT]^[GA]AC
HindII 199 GTCAAC GT[CT]^[GA]AC
KasI 1791 GGCGCC G^GCGCC
MluNI 2378 TGGCCA TGG^CCA
MscI 2378 TGGCCA TGG^CCA
MseI 338 TTAA T^TAA
MslI 831 CACTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1791 GGCGCC GG^CGCC
NheI 541 GCTAGC G^CTAGC
NsiI 935 ATGCAT ATGCA^T
PinAI 1809 ACCGGT A^CCGGT
SbfI 2038 CCTGCAGG CCTGCA^GG
SfoI 1791 GGCGCC GGC^GCC
SgrAI 1808 CACCGGTG C[GA]^CCGG[CT]G
SphI 3486 GCATGC GCATG^C
Sse8387I 2038 CCTGCAGG CCTGCA^GG
SspBI 2771 TGTACA T^GTACA
StuI 2112 AGGCCT AGG^CCT
Tru9I 338 TTAA T^TAA
XmnI 3337 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 3215 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
3478 CAGTGGCTG
Aor51HI 3012 AGCGCT AGC^GCT
3455 AGCGCT
BamHI 2907 GGATCC G^GATCC
3275 GGATCC
BlpI 3055 GCTGAGC GC^T[ACGT]AGC
3445 GCTGAGC
Bpu1102I 3055 GCTGAGC GC^T[ACGT]AGC
3445 GCTGAGC
BsaWI 1809 ACCGGT [AT]^CCGG[AT]
2544 TCCGGT
BsrBI 2461 GAGCGG CCGCTC(-3/-3)
3058 GAGCGG
BssSI 3328 CACGAG CACGAG(-5/-1)
2328 CTCGTG
CelII 3055 GCTGAGC GC^T[ACGT]AGC
3445 GCTGAGC
Eco47III 3012 AGCGCT AGC^GCT
3455 AGCGCT
EcoRV 367 GATATC GAT^ATC
578 GATATC
NaeI 2190 GCCGGC GCC^GGC
3381 GCCGGC
NcoI 590 CCATGG C^CATGG
1001 CCATGG
NgoMIV 2190 GCCGGC G^CCGGC
3381 GCCGGC
NspI 3044 ACATGT [GA]CATG^[CT]
3486 GCATGC
SexAI 975 ACCTGGT A^CC[AT]GGT
2396 ACCTGGT
SfiI 1687 GGCCCCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2947 GGCCCACCTGGCC
XcmI 618 CCAGGCTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
966 CCAACCACTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI